Category: csir-net-life-science-previous-year-questions-on Chromatography

75. A mixture of two proteins was subjected to following three chromatographic columns: (a) Cation exchange, (b) Size exclusion (Sephadex 100) and (c) Reverse phase. Following elution profiles were obtained. […]

74. Which one of the following techniques CANNOT be used to remove salt from a protein solution (A) Ultrafiltration (B) Ion exchange chromatography (C) Gel filtration chromatography (D) Dialysis The […]

73. You are studying the binding of proteins to the cytoplasmic face of cultured liver cells and have found a method that gives a good yield of inside-out vesicles from […]

72. Match the chromatographic technique from Group X with the appropriate elution conditions given in Group Y: Group X A. DEAE-Sephadex B. Chromatofocusing C. Phenyl Sepharose D. G-150 Sephadex Group […]

71. In which type of chromatography are ion-pairing agents used for elution? A. Hydrophobic interaction chromatography B. Reverse phase chromatography C. Ion exchange chromatography D. Immobilized metal anion chromatography Reverse […]

70. Nickel nitrilotriacetic acid columns are used in __________ chromatography. A. Ion exchange B. Affinity C. Size exclusion D. Reverse phase The correct answer is B. Affinity. Nickel nitrilotriacetic acid […]

69. Which one of the following purification steps always requires a high initial ionic strength in the sample? A. Ion exchange chromatography B. Hydrophobic interaction chromatography C. Chromatofocusing D. Gel […]

68. In a gel filtration chromatography: A. The large protein will be eluted first B. The small protein will be eluted first C. Both large and small will elute at […]

67. Which type of matrix is involved by Carboxymethyl cellulose among the following options: A. Anion-exchange matrix B. Plant cell wall constituent C. Cation-exchange matrix D. Gel filtration matrix Carboxymethyl […]

66. A mixture of proteins namely P, Q, R and S having molecular mass 50, 80, 120 and 150 kDa is applied on the Sephadex G-200 column. The order of […]