Q.105 Which of the following amino acid change (mutation) would MOST adversely affect the structure of an α-helix? (A) A valine residue changed to an isoleucine residue (B) A methionine residue changed to a proline residue (C) An aspartic acid residue changed to a glutamic acid residue (D) A histidine residue changed to an arginine residue

Proline Substitution Explained

Q.104 Which of the following statements is/are TRUE about JUXTACRINE signaling? I.  The ligand and the receptor engage in reciprocal signaling II.  Both the ligand and the receptor are membrane associated proteins III.  The ligand gets proteolytically cleaved after binding to the receptor (A) I only (B) II only (C) III only (D) I, II and III

 Juxtracrine Signaling True Statements

Q.103 From the point of view of the enzymatic reactions, which of the following DOES NOT belong here? (A) Telomerase (B) Reverse transcriptase (C) Taq polymerase (D) Primase

Enzymatic Reactions Odd One Out

Q.102 The term biological magnification refers to the increased levels of a toxin seen in successive trophic levels in a food web. Which one of the following options correctly states the reason(s) for the increment of a toxin in the ecosystem? (A) The toxin is highly toxic to primary producers, relatively less toxic to primary consumers, and non-toxic to secondary consumers. Thus, a higher level of toxin is seen in species representing higher trophic levels (B) The toxin cannot be degraded by microorganism and consequently persist in the environment for years (C) The toxin to begin with was not toxic or less toxic, but became more toxic by metabolism in the primary producers (D) Both (B) and (C)

Biological Magnification Toxin Increase Reasons

Q.101 Behavioral studies on animals have shown that there is relationship between mechanism of reproduction and male parental care (protecting eggs or the young ones). In aquatic invertebrates, fishes and amphibians for example, the species that practice internal fertilization rarely show male parental care while a majority of species that practice external fertilization tend to exhibit male parental care. This is likely due to (A) the male sex in species that practice internal fertilization are unable to defend against the predators (B) the male sex in species that practice internal fertilization live on female as parasite (C) the fact that the females of species that practice external fertilization die soon after laying the eggs (D) the certainty of paternity in species that practice external fertilization and this behavior is reinforced over generation by natural selection

External Fertilization Male Parental Care

Q.100 The birth control pills contain hormonal formulations that may either arrest the ovulation or prevent the fertilization of egg. Some of the formulations do both. Which one of the following combinations represents a formulation that is likely to affect the process of ovulation and fertilization? (A) Progesterone and estrogen (B) Prostaglandin and estrogen (C) Gonadotrophin and estradiol (D) Prolactin and estradiol

Progesterone Estrogen Combination Explained

Q.99 RNA is considered as the first genetic material to have evolved on the earth. Which one of the following properties of RNA is critical for its functioning as the genetic material in the absence of DNA and protein? (A) The presence of uracil as a base in place of thymine (B) The RNA is less stable than DNA; therefore RNA has higher probability to evolve as genetic material as compared to DNA (C) The single stranded RNA has a genotype as well as phenotype (D) RNA exists in 3 forms while DNA has only one form

RNA First Genetic Material

Q.98 In which one of the following organisms, it is easiest to distinguish mutations on adjacent base pairs of DNA through genetic recombination experiments? (A) Bacteriophages (B) Yeast (C) Escherichia coli (D) Bacillus subtilis

Bacteriophages vs Yeast, E. coli, B. subtilis

Q.97 In a newly discovered organism, normal development was unaffected when a few blastomeres were removed from 100-cell stage embryo. However, removal of five cells at the 1000-cell stage abolished the formation of kidney. Which one of the following options most accurately describes the type(s) of specification operating in the development of this organism? (A) Conditional specification only (B) Autonomous specification only (C) Conditional and autonomous specifications (D) Specification does not occur in this organism

Cell Stage Kidney Development Specification

Q.96 A genetic experiment was performed to map the gene(s) for eye colour in a newly-discovered moth species. Sex determination in this moth species: XY – male and XX – female. When blue-eyed males were mated to green-eyed females, all of both male and female progeny had green eyes. When these progeny were mated among themselves, about half of the males of the resulting second generation had blue eyes; however, all females were green-eyed. Which one of the following is consistent with the above data? (A) Multiple genes control eye colour in this moth species (B) Gene(s) for eye colour is located on the X chromosome (C) Gene(s) for eye colour is located on the Y chromosome (D) Gene(s) for eye colour may not be sex-linked

Moth Eye Color X-Linked

Q.95 Which of the following is TRUE about Kreb’s cycle? (A) Kreb’s cycle generates NADPH (B) The enzymes of Kreb’s cycle reside in the inter-membrane space of a mitochondria (C) It produces ATP, the energy currency of a cell (D) None of the above

Kreb’s Cycle NONE True

Q.94 The term innate behavior refers to an animal behavior (A) that is triggered by an environmental change (B) that is taught by the parent (C) that is developmentally fixed (D) that an organism learns on its own by “a hit-and trial” approach

Innate Behavior Developmentally Fixed

Q.93 Which one of the following hormones is INCORRECTLY paired with its function? (A) Melatonin – biological rhythm (B) Glucagon – increases blood glucose levels (C) Prolactin – stimulates milk secretion (D) Calcitonin – increases blood calcium level

 Calcitonin INCORRECTLY Paired

Q.92 Which one of the following examples represents an adaptation or a physiological activity that DOES NOT minimize the loss of body temperature of animals? (A) Feathers or fur (B) Fat layers in the adipose tissue (C) Shivering (D) Vasodilation

Vasodilation Does NOT Minimize Animal Body Temperature Loss

Q.91 In insect’s tracheal system, the transport of oxygen to the target tissue is done by (A) fine branches of air tubes extending to almost every cell (B) a liquid that fills the tracheal tube (C) a specialized set of cells that produce myoglobin (D) a specialized pigment

 Insect Tracheal System Oxygen Transport Fine Branches

Q.90 Which of the following is a main symptom of infection by Wuchereria bancrofti? (A) Swelling of limbs (B) Skin rashes (C) Blindness (D) Brain cyst

Wuchereria Bancrofti Main Symptom

Q.89 Which one of the following type of immune cells is responsible for graft rejection? (A) B cells (B) T cells (C) Macrophages (D) Eosinophils

 Immune Cells Responsible

Q.88 Extraembryonic tissues are derived from which one of the following? (A) Ectoderm (B) Endoderm (C) Trophectoderm (D) Mesoderm

 Extraembryonic Tissues Origin

Q.87 Which one of the following is characteristic of deuterostomes? (A) Radially symmetric body (B) Bilaterally symmetric body (C) Presence of well-defined digestive system (D) Formation of anus from blastopore

Deuterostome Characteristic

Q.86 Which one of the following is an example of eumetazoans? (A) Dictyostelium (B) Hydra (C) Sponges (D) Volvox

Eumetazoans Examples Hydra

Q.85 Match the pair of organisms in Group 1 with their characteristic interactions in Group 2 Group 1 P. Photoblepharon palpebratus and Vibrio fischeri Q. Pseudomonas and Bdellovibrio R. Aspergillus and Pseudomonas S. Thiobacillus ferrooxidans and Beijerinckia lactogenes Group 2 1. Mutualism 2. Symbiosis 3. Antagonism 4. Parasitism (A) P-2, Q-4, R-3, S-1 (B) P-2, Q-3, R-4, S-1 (C) P-4, Q-2, R-3, S-1 (D) P-2, Q-4, R-1, S-3

Microbial Interactions Matching

Q.84 An actively growing culture (20 ml) of E. coli (1 × 105 per ml) was mixed with a total of 100 T4 phage particles, grown further for 40 min and mixed with a few drops of chloroform. Under the conditions used, the generation time of E. coli is 30 min, the infection cycle of phage T4 is 20 min, and the burst size is 100. Assuming that each infection was a successful one, how many plaque forming units would you expect at the end of the experiment? (A) 104 (B) 103 (C) 105 (D) 106

E Coli T4 Phage Plaque Forming Units

Q.83 Based on their properties, match the “Genera” in Group 1 with those in Group 2 Group 1 P. Bacillus Q. Neisseria R. Rhizobium S. Caulobacter Group 2 1. Sarcina 2. Azotobacter 3. Hyphomicrobium 4. Clostridium (A) P-4, Q-1, R-2, S-3 (B) P-4, Q-1, R-3, S-2 (C) P-2, Q-4, R-1, S-3 (D) P-1, Q-4, R-2, S-3

Bacillus Neisseria Rhizobium Caulobacter Genera Properties Matching

Q.82 A new isolate of a facultative anaerobe utilizes either oxygen or pyruvate as terminal electron acceptor. This bacterium was grown either anaerobically with glucose as sole carbon source; or aerobically with lactose as the sole carbon source. Net increase in ATP production (per mole of the carbon source) during the aerobic growth would be (A) 2-fold (B) 4-fold (C) 19-fold (D) 38-fold

Net Increase in ATP Production During Aerobic Growth

Q.81 During replication of the E. coli chromosome, Okazaki fragments are produced from (A) only one of the strands of the circular genome (B) both the strands of the circular genome (C) one of the strands in one generation and the other strand in the next generation (D) both the strands of the circular genome provided that the heavy nitrogen (15N) is present in the medium

E. coli Okazaki Fragments Both Strands

Q.80 Match the names of investigators in Group 1 with their contributions in Group 2: Group 1 P. Joseph Lister Q. John Needham R. Elie Metchnikoff S. Lazaro Spallanzani Group 2 1. Role of phagocytosis in infection 2. Disproved spontaneous generation 3. Proved spontaneous generation 4. Use of agar as solidifying agent 5. Use of carbolic acid as disinfectant (A) P-5, Q-3, R-4, S-1 (B) P-5, Q-3, R-1, S-2 (C) P-4, Q-3, R-1, S-5 (D) P-3, Q-2, R-1, S-4

Matching Microbiology Pioneers and Contributions

Q.79 Which of the statements about Corynebacterium diphtheriae biology is NOT CORRECT? (A) All strains of C. diphtheriae are producers of diphtheria toxin (B) Diphtheria toxin production can be minimized by high concentration of iron in the medium (C) Diphtheria toxin inhibits protein synthesis (D) Diphtheria toxin is an A-B toxin secreted as a polypeptide of 62 kDa

Corynebacterium Diphtheriae Toxin Biology

Q.78 An actively growing culture of E. coli divides in about 20 min. Under laboratory conditions, time taken to replicate the entire genome of this bacterium would be about: (A) 20 min (B) 40 min (C) 10 min (D) 18 min

E. coli Genome Replication Time vs Division

Q.77 Which one of the following forms of the same DNA molecule would bind maximum ethidium bromide? (A) Negatively supercoiled (B) Covalently closed relaxed circle (C) Linear (D) Positively supercoiled

Which DNA Form Binds Maximum Ethidium Bromide

Q.76 Four Hfr strains of E. coli were generated from the same F+ strain. The Hfr strains donated markers in the following order: Strain 1: DQWMT; Strain 2: AXPTM; Strain 3: BNCAX; Strain 4: BDQWM The order of the markers in the original F+ strain is: (A) DQWMTPXACNB (B) AXPTMDQWBNC (C) BNCAXPTMDQW (D) BDQWMNCAXPT

Hfr Strains E. coli Marker Order

Q.75 Which one of the following immersion oils would you use to get the best resolution in a light microscope (with 100× objective)? (A) an oil with refractive index of 1.6 (B) an oil with refractive index of 1.5 (C) an oil with refractive index of 1.4 (D) an oil with refractive index of 1.3

Microscope Immersion Oil

Q.74 Which of the following are expected to be the abundant inhabitants of a nitrate- and sulfate-rich soil naturally depleted for oxygen? (A) Pseudomonas and Azotobacter (B) Pseudomonas and Desulfovibrio (C) Azotobacter and Thiobacillus (D) Nitrosomonas and Nitrobacter

Nitrate Sulfate Anoxic Soil Bacteria

Q.73 The cis–trans test can determine whether a gene codes for (A) an activator or a repressor (B) an RNA or a protein (C) a protein with the same or different amino acids (D) a diffusible or non-diffusible product

Cis-Trans Test Determines Diffusible Products

Q.72 The synthesis of an immunoglobulin in either a secretory or membrane bound form is governed by (A) allelic exclusion (B) class switching (C) differential RNA processing (D) affinity maturation

Immunoglobulin Forms

Q.71 A newly emerged variant of Influenza virus can be selectively propagated from the mixed population by addition of (A) Ganciclovir (B) Tamiflu (C) Interferon gamma (D) Neutralizing antibody

Influenza Variant Selection

Q.70 Catabolite repression allows cells to save energy by (A) inactivating catabolic enzymes (B) inhibiting synthesis of total RNA (C) regulating expression of genes required for utilization of less-efficient metabolites (D) inhibiting translation of mRNAs encoding catabolic enzymes

Catabolite Repression Energy Savings

Q.69 The active transport of solute in the cell is characterized by (A) its uptake along the concentration gradient utilizing energy (B) requirement of a carrier to support transport along the concentration gradient (C) chemical modification of the solute during its uptake (D) its uptake against the concentration gradient

Active Transport Against Concentration Gradient

Q.68 Which one of the following statements is NOT associated with contributions of Louis Pasteur? (A) Anthrax is caused by anthrax bacillus (B) Bacteria causing food spoilage come from air (C) The disease-causing organism must be isolated in pure culture (D) Bacteria cause the wine disease

Louis Pasteur Contributions

Q.67 To select for spontaneously arising histidine auxotrophs in a population, you would use a medium containing (A) Histidine and penicillin (B) Penicillin but no histidine (C) Histidine and lysozyme (D) Lysozyme but no histidine

Histidine Auxotroph Selection Medium

Q.66 Quinolones inhibit bacterial growth by targeting (A) DNA replication (B) mRNA translation (C) RNA polymerase (D) active transport of nutrients into the cell

Quinolones Inhibit Bacterial DNA Replication

Q.65 Which of the following statements are INCORRECT on ecological point of view? P. Primary succession involving xerosere is initiated in a wet habitat Q. Halones commonly found in electronic equipment are one of the active forces destroying the protective ozone layer in the stratosphere R. Sympatric speciation occurs when the new species evolves in geographic isolation from the parent species S. α-Diversity is the diversity of species within a habitat or community (A) P, Q (B) P, R (C) Q, R (D) Q, S

Incorrect Ecological Statements

Q.64 Identify the CORRECT statements. P. Specialized parenchymatous cells with tannins and crystals of calcium oxalate are termed as sclereids Q. The sieve elements of angiosperms are surrounded by companion cells and are essential component of phloem loading R. The exudation of water by guttation occurs through trichomes S. The bulliform cells control the unrolling and hygroscopic movement of grass leaves (A) P, Q (B) P, R (C) Q, S (D) P, S

Correct Statements in Plant Anatomy

Q.63 Which of the following statements are TRUE for the plastid genomes? P. Plastid genome is circular in nature with genome size of 120–160 kb Q. The plastid ribosomes are with sedimentation coefficient of 80S R. The gene for the small subunit of ribulose bisphosphate carboxylase (RubisCO) is located in the plastid S. rRNAs in the plastid genome are arranged in one transcription unit (A) P, Q (B) Q, S (C) R, S (D) P, S

True Statements About Plastid Genomes

Q.62 Identify the CORRECT statements for phylogenetic systems of classification P. The most popular phylogenetic system of classification is that of George Bentham and Joseph Dalton Hooker and was published in “Genera Plantarum” Q. A true phylogenetic system of classification was proposed by Adolf Engler and was published in “Die Natürlichen Pflanzenfamilien” R. The phylogenetic system of classification proposed by John Hutchinson appeared in “The Families of Flowering Plants” S. The origin of dicot from primitive monocot was proposed by Arthur Cronquist in his book “Systema Naturae” (A) Q, R (B) P, Q (C) R, S (D) P, S

Phylogenetic Systems of Classification

Q.61 Match the name of the disease with the causal organism Disease Causal organism P. False smut of rice 1. Plasmopara viticola Q. Ring rot of potato 2. Colletotrichum falcatum R. Red rot of sugarcane 3. Corynebacterium sepedonicum S. Downy mildew of grape 4. Ustilaginoidea virens 5. Erwinia amylovora 6. Synchytrium endobioticum (A) P-1 Q-5 R-2 S-4 (B) P-4 Q-3 R-2 S-1 (C) P-6 Q-2 R-4 S-1 (D) P-5 Q-3 R-2 S-4

Plant Diseases and Their Causal Organisms

Q.60 Match the plants with their seed storage proteins Plant Protein P. Rape seed 1. Kafirin Q. Pea 2. Vicillin R. Sorghum 3. Gliadin S. Wheat 4. Napin 5. Zein 6. Patatin (A) P-4 Q-3 R-5 S-2 (B) P-2 Q-3 R-6 S-1 (C) P-4 Q-2 R-1 S-3 (D) P-3 Q-2 R-4 S-5

Seed Storage Proteins of Plants

Q.59 Match the gene of interest for various aspects of crop improvement Gene insert P. bar Q. vip3A R. β-lcy S. gsh-II Aspects of crop improvement 1. Tolerance to heavy metals 2. Nutritional improvement with increased vitamin A 3. Insect resistance 4. Herbicide resistance 5. Delayed ripening 6. Resistance to fungal infection (A) P-4 Q-3 R-5 S-6 (B) P-4 Q-3 R-2 S-1 (C) P-2 Q-4 R-5 S-3 (D) P-4 Q-2 R-6 S-1

Genes of Interest in Crop Improvement

Q.58 Match the following plant secondary compounds with their uses and source plants Compounds P. Guggulusterol Q. Shikonin R. Ajmalicine S. Glycyrrhizin Uses 1. Anti-hypertensive 2. Anti-rheumatic 3. Dye 4. Sweetener 5. Anti-tumor 6. Anti-plaque Plant species i. Lithospermum erythrorhizon ii. Catharanthus roseus iii. Glycyrrhiza glabra iv. Commiphora wightii v. Swertia chirata vi. Coptis japonica (A) P-2-iv Q-3-i R-1-ii S-4-iii (B) P-3-iv Q-1-i R-5-ii S-6-iii (C) P-4-iv Q-3-i R-1-v S-2-vi (D) P-4-iii Q-2-ii R-5-i S-6-iv

Plant Secondary Compounds

Q.57 Which of the following statements are TRUE on DNA delivery methods during plant transformation? P. Single stranded nicks are made in T-DNA border repeat by the VirD1, VirD2 and VirD3 protein complex Q. virA gene products form the export apparatus on the membrane for the transfer of T-DNA R. Gold/Tungsten particles are used as microprojectiles in biolistic method S. Acceleration of DNA-coated microprojectiles is carried out with compressed CO2 (A) P, S (B) R, S (C) P, R (D) Q, S

DNA Delivery Methods in Plant Transformation

Q.56 Which of the following statements in photosynthesis are CORRECT? P. The absorption maxima for photosystem I (PS I) and PS II are 680 nm and 700 nm, respectively Q. Photosynthetic reaction centre contains 300 chlorophyll molecules and the release of one molecule of oxygen requires a minimum of 8 photons R. The non-photochemical quenching of excitation energy is enhanced by the presence of zeaxanthin S. The photochemical splitting of water occurs in PS I (A) P, Q (B) R, S (C) P, S (D) Q, R

Correct Statements in Photosynthesis

Q.55 A photoautotrophic micropropagation system can be established by increasing the (A) sucrose concentration in the culture medium (B) CO2 concentration in the culture medium (C) agar concentration in the culture medium (D) NH4+ concentration in the culture medium

Photoautotrophic Micropropagation

Q.54 Retrograde signaling involves communication of (A) nucleus to the chloroplast (B) endoplasmic reticulum to the nucleus (C) nucleus to the mitochondria (D) chloroplast to the nucleus

Retrograde Signaling

Q.53 The DNA content of a nucleus can be measured by (A) ESR Spectroscopy (B) FTIR Spectroscopy (C) Flow Cytometry (D) X-Ray Crystallography

Measuring DNA Content in Nuclei

Q.52 The transition of water molecule from liquid to glassy state during cryopreservation is termed as (A) Vitrification (B) Hyperhydricity (C) Cryoprotectant (D) Habituation

Cryopreservation Glassy State

Q.51 In which one of the following phases of cell cycle the drug colchicine exerts its effect? (A) G1 (B) G2 (C) S (D) M

Colchicine Effect on M Phase Cell Cycle

Q.50 One of the defense mechanisms adopted by plants for detoxification of heavy metals is the synthesis of (A) Phytochelatin (B) Calmodulin (C) Tubulin (D) Systemin

Plant Heavy Metal Detox

Q.49 During photorespiration the reactive oxygen species, H2O2, is produced in (A) Glyoxysome (B) Lysosome (C) Peroxisome (D) Dictyosome

hotorespiration H2O2 Production

Q.48 The mutagen ethidium bromide acts as a (A) Deaminating agent (B) Alkylating agent (C) Intercalating agent (D) Base analogue

Ethidium Bromide Mutagen

Q.47 The diploid chromosome number of an organism is 2n = 14. What would be the expected chromosome numbers in a nullisomic? (A) 12 (B) 13 (C) 15 (D) 16

Nullisomic Chromosome Number

Q.46 The stalk with which the ovule remains attached to the placenta is called (A) Micropyle (B) Chalaza (C) Funiculus (D) Hilum

Ovule Stalk Attachment Placenta

Q.45 Scatchard analysis of ligand-receptor interaction yielded the graph shown below. The affinity of the ligand-receptor interaction can be obtained from Bound / Free Bound (molar) (A) Y intercept (B) X intercept (C) Slope of the line (D) Product of X intercept and Y intercept

Scatchard Analysis Explained

Q.44 Match the following Group I P) Tyrosine hydroxylation Q) Tyrosine iodination R) Tyrosine phosphorylation S) Tyrosine oxidation Group II 1) Thyroxine 2) T cell Receptor 3) DOPA 4) Estradiol receptor 5) Epinephrine 6) Melanin 7) Endorphin 8) Serotonin (A) P-1, Q-6, R-5, S-4 (B) P-5, Q-7, R-4, S-8 (C) P-2, Q-5, R-3, S-4 (D) P-3, Q-1, R-2, S-6

Matching Tyrosine Modifications with Biological Molecules

Q.43 Collagen, α-keratin and tropomyosin have common structural features. They are P) disulfide bridges to neighboring proteins. Q) repeating sequences of amino acids R) a high β-sheet content S) superhelical coiling (A) P, Q (B) Q, R (C) Q, S (D) P, R

Common Structural Features

Q.42 Match the following: Group I Group II P) Polynucleotide kinase 1) ATPase Q) Fluoride 2) GTPase R) Ras 3) Transketolase S) lac operon 4) Enolase 5) 5′ end of DNA 6) 3′ end of DNA 7) Only positive regulation 8) Positive and negative regulation Options: (A) P-5, Q-4, R-2, S-8 (B) P-6, Q-3, R-1, S-7 (C) P-4, Q-2, R-1, S-6 (D) P-1, Q-7, R-5, S-3

Match Enzymes and Genetic Elements

Q.41 Four identical PCR reactions were carried out in tubes named I, II, III and IV. Besides the usual mix of dNTPs, each of the tubes respectively contained γ-32P dATP, β-32P dATP, α-32P dATP and α-32P rNTP. Which one of the tubes will have radiolabeled PCR product? Options: (A) Tube I (B) Tube II (C) Tube III (D) Tube IV

Which Radiolabeled Nucleotide Produces Labeled PCR Product?

Q.40 Three samples of antibodies were electrophoresed under denaturing and reducing conditions on a 15% acrylamide gel, followed by staining with Coomassie blue dye. Samples 1, 2 and 3 showed two, three and four stainable bands respectively. Which one of the following conclusions can be made from these observations? Options: (A) Sample 1 is IgG, 2 is IgA and 3 is IgM (B) Sample 1 is IgA, 2 is IgM and 3 is IgG (C) Sample 1 is IgG, 2 is IgM and 3 is IgA (D) Sample 1 is IgA, 2 is IgG and 3 is IgM

Identification of Antibody Classes

Q.39 Match the items in Group I with those in Group II Group I Group II P) Progesterone 1) Peptide Q) Dopamine 2) Fatty acid R) Vasopressin 3) Carbohydrate S) Prostaglandin 4) Catecholamine 5) Eicosanoid 6) Steroid Options: (A) P-3, Q-4, R-1, S-2 (B) P-6, Q-4, R-1, S-5 (C) P-3, Q-5, R-4, S-1 (D) P-6, Q-5, R-1, S-4

Match Hormones and Signaling Molecules

Q.38 In the following enzyme-catalyzed reaction which follows Michaelis–Menten kinetics: E + S   ⇌ k1     k−1   ES  →k2  E + P Km is equal to Options: (A) k−1 / (k1k2) (B) (k1k2) / k−1 (C) k1 / (k2 + k−1) (D) (k2 + k−1) / k1

Michaelis Constant (Kₘ)

Q.37 Two purified DNA samples A and B contain equal number of base pairs. Each of these DNA samples has one site each for EcoRI and BamHI restriction enzymes. Complete digestion with both the enzymes yielded 3 DNA bands and 2 DNA bands respectively for A and B upon electrophoresis of the digestion products. Which one of the following explains the observation? Options: (A) A is circular DNA and B is linear (B) B is circular DNA and A is linear (C) A is circular DNA and B could be linear or circular (D) B is circular DNA and A could be linear or circular

Restriction Enzyme Digestion of DNA

Q.36 Match P, Q, R and S with the appropriate numbers 1 to 6 on the right Left Right P) Basophils 1) Perforin Q) T cells 2) Phagocytosis R) B cells 3) Albumin S) Neutrophils 4) Macroglobulin 5) Fc receptors for IgE 6) Plasma cells Options: (A) P-5, Q-1, R-6, S-2 (B) P-1, Q-2, R-3, S-4 (C) P-3, Q-4, R-5, S-1 (D) P-2, Q-6, R-1, S-3

Match the Immune Cells with Their Functions

Q.35 Valinomycin, a cyclic peptide antibiotic, facilitates the transport of which one of the following ions? Options: (A) K+ (B) Ca2+ (C) Na+ (D) H+

Valinomycin Ion Transport

Q.34 A mixture of four proteins of pIs 11, 7, 5 and 3 are loaded on a DEAE anion-exchange column equilibrated with low ionic strength buffer of pH 8. Which of the four proteins would be expected to be retained on the column? Options: (A) Protein with pI 11 but not the others (B) Proteins with pIs 11 and 7 but not 5 and 3 (C) Proteins with pIs 7, 5 and 3 (D) Protein with pI 7 but not the others

DEAE Anion Exchange Protein Retention

Q.33 In humans, the largest energy reserve is Options: (A) liver glycogen (B) muscle glycogen (C) blood glucose (D) adipose tissue triacylglycerol

 Human Largest Energy Reserve

Q.32 Cyanide poisoning is due to its direct inhibition of Options: (A) Electron transport chain (B) Fatty acid biosynthesis (C) Fatty acid oxidation (D) Nucleic acid biosynthesis

Cyanide Poisoning Mechanism

Q.31 The polarity of water molecule is due to Options: (A) its tetrahedral structure (B) bonding electrons being attracted more to oxygen (C) bonding electrons being attracted more to hydrogen (D) its weak electrolytic property

Water Molecule Polarity Cause

Q.30 Horseradish peroxidase and alkaline phosphatase are the two enzymes commonly utilized as reagents in ELISA, because these enzymes Options: (A) are colored proteins (B) are very small (C) have high turnover number (D) bind to ELISA plates

ELISA Enzymes HRP & Alkaline Phosphatase

Q.29 Cytochrome C is normally found in the inner mitochondrial membrane. It is released into the cytoplasm during Options: (A) Apoptosis (B) Necrosis (C) Cell differentiation (D) Cell proliferation

Cytochrome C Release

Q.28 Which one of the following enzymes fixes CO2 into organic form? Options: (A) Ribulose 5-phosphate kinase (B) Ribulose 1,5-bisphosphate carboxylase (C) Pyruvate dehydrogenase (D) Carbonic anhydrase

CO2 Fixation Enzyme

Q.27 The activation of the complement components occurs via three distinct pathways. Which of the following component(s) is specific to the Alternate Pathway? Options: (A) Factor B and D (B) Mannose binding protein (C) C1q r2 s2 (D) C2

Complement Alternate Pathway Components

Q.26 Which one of the following DOES NOT inhibit protein biosynthesis? Options: (A) Puromycin (B) Chloramphenicol (C) Cycloheximide (D) Oligomycin

Protein Biosynthesis Inhibitors

Consider the reaction (CH3)(C2H5)(C6H5)C–Br+  KOH (aq)→ (CH3)(C2H5)(C6H5)C–OH Q.25 If the concentration of KOH in the reaction mixture is doubled, the rate of the reaction will be Options: (A) decreased to one-half (B) the same (C) increased by two-times (D) increased by four-times

Effect of KOH Concentration

Consider the reaction (CH3)(C2H5)(C6H5)C–Br+  KOH (aq) → (CH3)(C2H5)(C6H5)C–OH Q.24 The above reaction is an example of Options: (A) addition reaction (B) bimolecular elimination reaction (E2) (C) unimolecular substitution reaction (SN1) (D) bimolecular substitution reaction (SN2)

SN1 Reaction Example

The solubility products of FeS, ZnS, CuS and HgS are 1.0 × 10−19, 4.5 × 10−24, 4.0 × 10−38 and 3.0 × 10−53 respectively. Q.23 The concentration of S2−, at which FeS begins to precipitate from the mixture having 0.1 M Fe2+ is Options: (A) 1.0 × 10−17 M (B) 1.0 × 10−18 M (C) 1.0 × 10−19 M (D) 1.0 × 10−20 M

Concentration of S²⁻ Required

The solubility products of FeS, ZnS, CuS and HgS are 1.0 × 10−19, 4.5 × 10−24, 4.0 × 10−38 and 3.0 × 10−53 respectively.  Q.22 H2S is passed through an aqueous solution containing all the four metal ions. The metal ion that precipitates first is Options: (A) Fe2+ (B) Zn2+ (C) Cu2+ (D) Hg2+

Which Metal Ion Precipitates First When H₂S Is Passed?

Q.21 The ions with lowest and highest radii among O2−, F−, Na+ and Mg2+ are respectively, Options: (A) Mg2+ and O2− (B) O2− and F− (C) O2− and Mg2+ (D) Na+ and Mg2+

Lowest and Highest Ionic Radii

Q.20 According to crystal field theory, the electronic configuration of [Ti(H2O)6]3+ in the ground state is Options: (A) eg1 t2g0 (B) t2g0 eg1 (C) eg0 t2g1 (D) t2g1 eg0

Electronic Configuration of [Ti(H₂O)₆]³⁺

Q.19 According to the molecular orbital theory, bond order for H2+ species is Options: (A) 0.5 (B) 1.0 (C) 1.5 (D) 2.0

Bond Order of H2+

Q.18 A molecular electronic excited state has a lifetime of 10−9 s. The uncertainty in measuring the frequency (Hz) of the electronic transition is approximately Options: (A) h⁄4π × 109 (B) h⁄4π × 10−9 (C) 1⁄4π × 10−9 (D) 1⁄4π × 109

Uncertainty in Frequency

Q.17 Consider the following equilibrium: SO2 (g) + ½ O2 (g) ⇌ SO3 (g),   ΔH = −23.5 kcal mol−1 The formation of SO3 is favoured by Options: (A) compression and decreasing the temperature (B) compression and increasing the temperature (C) expansion and increasing the temperature (D) expansion and decreasing the temperature

Formation of SO3

Q.16 The correct order of acidity is Options: (A) C6H5COOH < CH3COOH < C6H5OH < C2H5OH (B) CH3COOH < C6H5COOH < C2H5OH < C6H5OH (C) C2H5OH < C6H5OH < C6H5COOH < CH3COOH (D) C2H5OH < C6H5OH < CH3COOH < C6H5COOH

Correct Order of Acidity

Q.15 The shape of PCl5 is Options: (A) tetrahedral (B) square planar (C) trigonal bipyramidal (D) square pyramidal

PCl5 Molecular Shape

Q.14 Which one of the following compounds is known as silanes? Options: (A) Silicon hydrides (B) Silicon halides (C) Silicon hydroxides (D) Silicon oxides

Silanes Definition Chemistry

Q.13 Trouton’s rule is obeyed by Options: (A) hydrogen (B) methanol (C) benzene (D) acetic acid

Trouton’s Rule Obeyed By

Q.12 The major product for the following reaction is

Major Product of Friedel–Crafts Alkylation

Q.11 Electrophile among the following is Options: (A) NH3 (B) SO3 (C) NO2 (D) CH≡C−

Electrophile Among the Following

Q.10 The fuel consumed by a motorcycle during a journey while traveling at various speeds is indicated in the graph below. Fuel Consumption: kilometres per litre Speed: kilometres per hour The distances covered during four laps of the journey are listed in the table below: Lap Distance (kilometres) Average speed (kilometres per hour) P 15 15 Q 75 45 R 40 75 S 10 10 From the given data, we can conclude that the fuel consumed per kilometre was least during the lap Options: (A) P (B) Q (C) R (D) S

Fuel Consumption per Kilometre

Q.9 Three friends, R, S and T shared toffee from a bowl. R took 1/3rd of the toffees, but returned four to the bowl. S took 1/4th of what was left but returned three toffees to the bowl. T took half of the remainder but returned two back into the bowl. If the bowl had 17 toffees left, how many toffees were originally there in the bowl? Options: (A) 38 (B) 31 (C) 48 (D) 41

Three Friends Sharing Toffees

Q.8 Given that f(y) = |y| / y, and y is any non-zero real number, the value of f(q) − f(−q) is Options: (A) 0 (B) −1 (C) 1 (D) 2

Value of |f(q) − f(−q)|

Q.7 The sum of n terms of the series 4 + 44 + 444 + … is Options: (A) (4/81) [10n+1 − 9n − 1] (B) (4/81) [10n − 9n − 1] (C) (4/81) [10n+1 − 9n − 10] (D) (4/81) [10n − 9n − 10]

Sum of n Terms of the Series

Q.6 The horse has played a little known but very important role in the field of medicine. Horses were injected with toxins of diseases until their blood built up immunities. Then a serum was made from their blood. Serums to fight with diphtheria and tetanus were developed this way. It can be inferred from the passage, that horses were Options: (A) given immunity to diseases (B) generally quite immune to diseases (C) given medicines to fight toxins (D) given diphtheria and tetanus serums

Horses in Medicine Reading Inference

Q.5 There are two candidates P and Q in an election. During the campaign, 40% of the voters promised to vote for P, and the rest for Q. However, on the day of election, 15% of the voters went back on their promise to vote for P and instead voted for Q. 25% of the voters went back on their promise to vote for Q and instead voted for P. Suppose, P lost by 2 votes, then what was the total number of voters? Options: (A) 100 (B) 110 (C) 90 (D) 95

Election Voters Problem

Q.4 Choose the most appropriate word from the options given below to complete the following sentence: It was her view that the country’s problems had been __________ by foreign technocrats, so that to invite them to come back would be counter-productive. Options: (A) identified (B) ascertained (C) exacerbated (D) analysed

Country’s Problems Sentence Completion

Q.3 Choose the word from the options given below that is most nearly opposite in meaning to the given word: Frequency Options: (A) periodicity (B) rarity (C) gradualness (D) persistency

Frequency Antonym Question

Q.2 Choose the most appropriate word from the options given below to complete the following sentence: Under ethical guidelines recently adopted by the Indian Medical Association, human genes are to be manipulated only to correct diseases for which __________ treatments are unsatisfactory. Options: (A) similar (B) most (C) uncommon (D) available

Human Genes Manipulation Sentence Completion

Q.1 The question below consists of a pair of related words followed by four pairs of words. Select the pair that best expresses the relation in the original pair: Gladiator : Arena Options: (A) dancer : stage (B) commuter : train (C) teacher : classroom (D) lawyer : courtroom

Gladiator Arena Analogy Question

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