Louis Pasteur revolutionized microbiology through germ theory, vaccines, and pasteurization. This SEO article identifies the statement NOT associated with him in Q.68, with full explanations for exams.

Correct Answer

The correct answer is (C) The disease-causing organism must be isolated in pure culture.

Pure culture isolation was Robert Koch’s postulate (1880s), requiring microbes to be grown separately to prove causation. Pasteur focused on linking microbes to disease through experiments but did not emphasize pure cultures.

Option Breakdowns

(A) Anthrax is caused by anthrax bacillus

Pasteur developed anthrax vaccine (1881) after confirming Bacillus anthracis causes disease in animals.
His field trials proved germ theory application.​

(B) Bacteria causing food spoilage come from air

Pasteur’s swan-neck flask experiments showed microbes in air cause spoilage/fermentation.
Disproved spontaneous generation.​

(C) The disease-causing organism must be isolated in pure culture

Koch’s second postulate formalized pure culture requirement for disease proof.
Pasteur used mixed cultures effectively.​

(D) Bacteria cause the wine disease

Pasteur’s 1860s work identified bacteria spoiling wine/beer, leading to pasteurization.
Saved French wine industry.​