Category: CSIR NET Life Science Previous Year Questions and Solution on TRANSLATION Inhibitors

Category: CSIR NET Life Science Previous Year Questions and Solution on TRANSLATION Inhibitors

CSIR NET Life Science Previous Year Questions and Solution on TRANSLATION Inhibitors

Ribosomes prepared from a bacterium were fractionated by sucrose density gradient centrifugation (panel i) to separate the 30S, SOS and 70S populations. When the ribosome prepuation was incubated individually with either elongation factor-G (EF-G), or a newly identified protein X. Or GTP, the profile remained unchanged. Likewise. no changes were seen in the profile when the ribosomal preparation was incubated with EF-G + GTP or protein X + GTP. However, when the ribosomal preparation was incubated with protein X. EF.G and GTP together, itresulted in a change of profile which showed a decrease of the 70S peak area and increase in the peak areas for 30S and 50S (panel ii).   choose the option that defines a correct conclusion from the observations. (1) Protein X is an anti-association factor which functions in the presence of EF-G and GTP (2) Protein X is a dissociation factor which functions in the presence of EF-G and GTP (3) Protein X binds GTP (4) EF-G is known to bind GTP, hence it can be concluded that the effect of GTP is through EF-G and protein X does not bind GTP.

 Protein X Functions as a Ribosome Dissociation Factor in the Presence of EF-G and GTP: Insights from Sucrose Gradient Profiles

12. When one isolates ribosomes from bacterial lysate, apart from 70S. 50S and 30S ribosomal subunits. One also finds a small population of 100S, 130S and 150S sub-units. EDTA dissociates these larger ribosomal subunits into 50S and 30S. suggesting that they have both the subunits. upon addition of cations they reassociate into 70S. but none of the other forms could be detected. What is the reason for not obtaining the >70S forms? (1) The effects of EDTA cannot be reversed by the addition of cations. (2) 190S, 130S and150S are modified form of ribosomes that are irreversibly damaged by EDTA. (3) 100S, 130S etc. represent polysome that cannot be reassembled denovo without other cellular components. (4) They are obtained as an experimental artifact in - preparations of ribosomes

Understanding Why >70S Ribosomal Forms Are Not Recovered After EDTA Dissociation and Reassembly

How to Interpret Polysome Profiles for CHP, LTM, and PTM Translation Inhibitors

10. Among the following which antibiotic is inhibitor of 80S ribosome? (1) Tetracycline (2) Streptomycin (3) Cycloheximide(4) Chloramphenicol

Cycloheximide: The Antibiotic That Specifically Inhibits the Eukaryotic 80S Ribosome

Which one of the following statements is correct in the context of erythromycin-mediated inhibition of protein synthesis in bacteria? (1) Erythromycin inhibits protein synthesis at the step of elongation, but it nonetheless allows translation of the first few codons. (2) Erythromycin inhibits protein synthesis at the step of elongation, and it prevents formation of even the first peptide bond. (3) Erythromycin inhibits formation of the translation initiation complex. (4) Erythromycin is toxic to bacteria because it results in initiation of protein synthesis with elongatortRNAs.

 Erythromycin Inhibits Bacterial Protein Synthesis by Blocking Elongation After Initial Codons Are Translated

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