![Q.42 Determine the correctness or otherwise of the following Assertion [a] and the Reason [r] Assertion [a]: A genetically engineered rice that produces beta-carotene in the rice grain is called Golden rice Reason [r]: Enabling biosynthesis of provitamin A in the rive endosperm gives a characteristic yellow orange color (A) Both [a] and [n] are false (B) Both [a] and [r] are true but is not the correct reason for [a] (C) Both [a] and are true and is the correct reason for [a] (D) [A] is true but [r] is false](https://www.letstalkacademy.com/wp-content/uploads/2026/01/slide_42-10.png)
![Q.41 Which of the following statements about gene therapy are CORRECT? [P] Affected individuals, but not their progeny, can be cured through germline gene theraphy [Q] Affected individuals, as well as their progeny, can be cured through germline gene therapy [R] Affected individuals, but not their progeny, can be cured through somatic gene theraphy [S] Affected individuals, as well as their progeny, can be cure through somatic gene therapy (A) P and R only (B) P and S only (C) Q and R only (D) Q and S only](https://www.letstalkacademy.com/wp-content/uploads/2026/01/slide_41-10.png)
![Q.40 Determine the correctness or otherwise of the following Assertion [a] and the Reason [r] Assertion [a]: A typule and its immediate descendant cells are specialized and are called toipotent Reason [C]: Totipotent cells retain the capacity to differentiate into only a few cell types (A) Both [a] and [r] are false (B) Both [a] and [r] are true but [r] is not the correct reason for [a] (C) Both [a] and [r] are true and [r] is the correct reason for [a] (D) [a] is true but is false](https://www.letstalkacademy.com/wp-content/uploads/2026/01/slide_40-10.png)
![Q.39 Which of the following strategies are used by cells for metabolic regulation! [P] Phosphorylation- dephosphorylation [Q] Allostery [R| Feedback inhibition (A) P and Q only (B) P and R only (C) Q and R only (D) P, Q and R](https://www.letstalkacademy.com/wp-content/uploads/2026/01/slide_39-10.png)
![Q.No. 38 Match sub-cellular organelles listed in Group I with their features listed in Group II: Group I Group II P] Mitochondrion 1. Single-membrane enclosed Q] Chloroplast 2. Double-membrane enclosed R] Nucleus 3. Maternal inheritance S] Endoplasmic reticulum 4. Endosymbiotic origin Options: P-1, Q-4, R-2, S-3 P-2, Q-3, R-4, S-1 P-3, Q-4, R-2, S-1 P-3, Q-1, R-4, S-2](https://www.letstalkacademy.com/wp-content/uploads/2026/01/slide_38-10.png)
![Q.37 The schematic of a plasmid with a gap in one of the strands is shown below: Which of the following enzyme(s) is/are required to fill the gap and generate a covalently closed circular plasmid? [P] DNA ligase [Q] Alkaline phosphatase [R] DNA polymerase [S] Polynucletoide Kinase](https://www.letstalkacademy.com/wp-content/uploads/2026/01/slide_37-10.png)
![Q.36 Determine the correctness or otherwise of the following Assertion (a) and the Reasonv[r]. Assertion [a]: Embryonic stem cells are suitable for developing knockout mice Reason [r]: Homologous recombination is more frequent in embryonic stem cells than that in somatic cells (A) Both [a] and [r] are false (B) Both [a] and [r] are true, and [r] is the correct reason for [a] (C) Both [a] and [r] are true, but [r] is not the correct reason for [a] (D) [a] is true, but [r] is false](https://www.letstalkacademy.com/wp-content/uploads/2026/01/slide_36-10.png)
![Q.35 Determine the correctness or otherwise of the following Assertion [a] and the Reason [r]. Assertion [a]: Dam methylase protects E. coli DNA from phage endonucleases Reason [r]: E. coli Dam methylase methylates the adenosine residue in the sequence "GATC" (A) Both [a] and [r] are true and [r) is the correct reason for [a] (B) Both [a] and [r) are true but [r] is not the correct reason for [a] (C) Both [a] and [r] are false (D) [a] is false but [r] is true](https://www.letstalkacademy.com/wp-content/uploads/2026/01/slide_35-10.png)
![Q.No. 34 A list of pathogens (Group I) and a list of anti-microbial agents (Group II) used to treat their infections are given below. Match the pathogens with the corresponding anti-microbial agents. Group I Group II P] Influenza A virus 1. Isoniazid Q] Fungus 2. Amantadine R] Plasmodium 3. Fluconazole S] Mycobacterium 4. Artemisinin 5. Iodoquinol Options: P-4, Q-3, R-2, S-5 P-5, Q-2, R-4, S-1 P-2, Q-3, R-4, S-1 P-2, Q-3, R-1, S-5](https://www.letstalkacademy.com/wp-content/uploads/2026/01/slide_34-10.png)
![Q.32 Some of the cytokinins used in plant tissue culture media are given below: [P] ВАР [Q Zeatin [R] Kinetin [S]2iP Which of these are synthetic analogs? (A) P and Q only (B) Q and S only (C) R and S only (D) P and R only](https://www.letstalkacademy.com/wp-content/uploads/2026/01/slide_32-10.png)
![Q.No. 29 Which of the following statements are CORRECT about eukaryotic cell cycle? [P] CDKs can phosphorylate proteins in the absence of cyclins [Q] CDKs can be inactivated by phosphorylation [R] Degradation of cyclins is required for cell cycle progression [S] CDKs are not involved in chromosome condensation (A) P and R only (B) P and S only (C) P, Q and R only (D) Q and R only](https://www.letstalkacademy.com/wp-content/uploads/2026/01/slide_29-11.png)
![Q.No. 28 Which of the following statements about immune response are CORRECT? [P] T cells are activated by antigen-presenting cells [Q] Foreign peptides are not presented to helper T cells by Class II MHC proteins [R] Dendritic cells are referred to as professional antigen-presenting cells (A) P and R only (B) P and Q only (C) Q and R only (D) P, Q and R](https://www.letstalkacademy.com/wp-content/uploads/2026/01/slide_28-11.png)
![Q.No. 25 A variable Y is a function of t. Given that Y(t = 0) = 1 and Y(t = 1) = 2, dY/dt in the interval t = [0, 1] can be approximated as __________.](https://www.letstalkacademy.com/wp-content/uploads/2026/01/slide_25-11.png)
![Q.No. 23 The largest eigenvalue of the matrix [ 4 1 -2 1 ] is __________.](https://www.letstalkacademy.com/wp-content/uploads/2026/01/slide_23-11.png)
![Q.19 The elemental composition of dry biomass of a yeast species is CH1.6O0.4N0.25O0.0024P0.017. The contribution of carbon to the dry biomass is _______ % (round off to 2 decimal places). [Given: atomic weights of H, C, N, O, P and S are 1, 12, 14, 16, 31 and 32 respectively]](https://www.letstalkacademy.com/wp-content/uploads/2026/01/slide_19-11.png)
![Q.15 Amino acid sequences of cytochrome e and ribulose S-phosphate epimerase from 40 organisms were chosen and phylogenetic trees were obtained for each of these two protein families Determine the correctness or otherwise of the following Assertion [a] and the Reason 1 Assertion (a): The two trees will not be identical Reason (r): The nature and frequency of mutations in the two families are different (A) Both [a] and are true and [r] is the correct reason for [a] (B) Both [a] and [r] are true but is not the correct reason for [a] (C) Both [a] and [r] are false. (D) [a] is false but [r] is true](https://www.letstalkacademy.com/wp-content/uploads/2026/01/slide_15-11.png)
![Q.14 Which of the following are energy transducing membranes? [P] Plasma membrane of bacteria [Q] Inner membrane of chloroplasts [R] Inner membrane of mitochondria (A) P and Q only (B) P and R only (C) Q and R only (D) P, Q and R](https://www.letstalkacademy.com/wp-content/uploads/2026/01/slide_14-11.png)
![Q.12 Which of the following types of molecules act as biological catalysts? [P] Protein [Q] RNA [R] Phospholipid (A) Pand only (B) P and R only (C) Q and R only (D) P, Q and R](https://www.letstalkacademy.com/wp-content/uploads/2026/01/slide_12-11.png)
![Q.5 Determine the correctness or otherwise of the following Ascention (a) and the Reason (r) regarding mammalian cells. Assertion (a): Cells use Ca2+, and not Na+, for cell-to-cell signalling Reason[r): In the cytosol, concentration of Na+ is lower than that of Ca2+ ? (A) Both (a) and (r) are true and (r) is the correct reason for [a]. (B) Both (a) and rare true but [c] is not the correct reason for (a). (C) Both (a) and care false (D) [a] is true but is false.](https://www.letstalkacademy.com/wp-content/uploads/2026/01/slide_5-11.png)
![Q.3 Which of the following statements are CORRECT? [P] Viruses can play a role in causing human Cancer [Q] A tumor suppressor gene can be turned off without any change in its DNA sequence [R] Alteration in miRNA expression levels contributes to the development of cancer (A) P and only (B) Q and only (C) P and only (D) P. and R](https://www.letstalkacademy.com/wp-content/uploads/2026/01/slide_3-11.png)
![Q.9 For a matrix M = [mij]; i, j = 1,2,3,4, the diagonal elements are all zero and mij = -mji. The minimum number of elements required to fully specify the matrix is (A) 0 (B) 6 (C) 12 (D) 16](https://www.letstalkacademy.com/wp-content/uploads/2026/01/slide_9.jpg)
Q.No. 55 The mitochondrial electron transfer chain oxidizes NADH with oxygen being the terminal electron acceptor. The redox potentials for the two half-reactions are given below: NAD+ + H+ + […]
Category: GATE Biotechnology 2020 Previous Year Papers with Solutions
GATE Biotechnology 2020 Previous Year Papers with Solutions
GATE Biotechnology 2020 Previous Year Papers with Solutions
Globin Sequence Algorithm Sensitivity Calculation
Q.No. 54 An algorithm was designed to find globins in protein sequence databases. A database which has 78 globin sequences was searched using this algorithm. The algorithm retrieved 72 sequences […]
GATE Biotechnology 2020 Previous Year Papers with Solutions
Free Energy Change for ATP Synthesis at 37°C
Q.53 The concentrations of ATP, ADP and inorganic phosphate in a cell are 2.59, 0.73and 2.72 mM, respectively. Under these conditions, free energy change for thesynthesis of ATP at 37 […]
GATE Biotechnology 2020 Previous Year Papers with Solutions
Protein A Protein B Covalent Complex
Q.No. 52 Protein A and protein B form a covalent complex. Gel filtration chromatography of this complex showed a peak corresponding to 200 kDa. SDS-PAGE analysis of this complex, with […]
GATE Biotechnology 2020 Previous Year Papers with Solutions
Infinite Series Sum
Q.No. 51 An infinite series S is given as: S = 1 + 2/3 + 3/9 + 4/27 + 5/81 + … (to infinity) The value of S is _____________ […]
GATE Biotechnology 2020 Previous Year Papers with Solutions
Maximize Quadratic Function f(X) = 4X – X²
Q.No. 50 A function f is given as: f(X) = 4X − X2 The function f is maximized when X is equal to _____________. Maximize Quadratic Function f(X) = 4X […]
GATE Biotechnology 2020 Previous Year Papers with Solutions
Monod Model Chemostat
Q.No. 49 Growth of an organism on glucose in a chemostat is characterized by Monod model with specific growth rate = 0.45 h-1 and Ks = 0.5 g/L. Biomass from […]
GATE Biotechnology 2020 Previous Year Papers with Solutions
Total Kinetochores in Human Fibroblast Cell Culture
Q.No. 48 Assume that a cell culture was started with five human fibroblast cells. Two cells did not divide even once whereas the other three cells completed three rounds of […]
GATE Biotechnology 2020 Previous Year Papers with Solutions
Number of Unique DNA Sequences Encoding
Q.47 The amino acid sequence of a peptide is Phe–Leu–lle–Met–Ser–Leu. The number ofcodons that encode the amino acids present in this peptide is given below:Phe: 2 codonsLeu: 6 codonslle: 3 […]
GATE Biotechnology 2020 Previous Year Papers with Solutions
System of Linear Equations No Solution When C Equals What?
Q.No. 46 The system of linear equations: cx + y = 5 3x + 3y = 6 has no solution when c is equal to ____________________. The system of linear […]
