CSIR NET Life Science Previous Year Questions and Solution on MOLECULAR TECHNIQUES
11. An in vitro translation system capable of incorporating ~8 amino acids was programmed to translate a single mRNA that codes for an alanine-rich (35% alanine with uniform distribution of alanine) protein of 275 amino acids (~30kDa) including a hexa-histidine tag at the c-terminal end of the protein. The protein possesses three methionine residues at […]
10. A reporter cell line with stably integrated retroviral promoter-luciferase construct was transfected with an expression vector for a cellular protein. The protein seems to regulate the activation of retroviral promoter as analyzed by luciferase activity assay. Which one of the following techniques will you use to show “in vivo” recruitment of the cellular protein […]
9. A researcher wanted to identify the enhancer sequences of a newly discovered gene. Shown below are the relevant regions of some of the reporter constructs the researcher designed to identify the enhancer. Which of the above constructs can be used to identify the enhancer? (1) A only […]
The data from an S1 nuclease mapping experiment for a transcript mfg1 using a 5′-end labelled probe are shown below. Following interpretations were made: A. The liver RNA is 500 bp in length B. The start site of the liver mfgl transcript is 500 bp downstream of the 5′ end of probe. C. The kidney […]
7. Run off transcription assays were performed to establish the specificity of three novel sigma factors for their promoters. Result of the experiments are shown below: Following inferences were made from these results: A. σA initiates transcription from P? and σB from P1 B. σC can initiates transcription from both promoters. C. σB Prevents initiation […]
6. A researcher was working with three proteins, A, B and C which may have potential roles in gene expression. In order to validate the hypothesis, EMSA (electrophoretic mobility shift assay) was performed. The purified proteins were allowed to bind with a labelled DNA and the results obtained after autoradiography as shown below. The following […]
Deletion analysis of a promoter region of a gene was carried out to identify the regulatory elements in it. In the figure below, the filled boxes denote the areas of deletion and the observed activities (in arbitrary units) of the promoter are as shown.Based on the observations, following statements were made: F. The region between-100 […]
In order to identify the regulatory regions of a novel promoter sequence shown above, four 150 bp deletion constructs were made in a luciferase reporter system as indicated above in boxes A to D. After transfection, the observed level of promoter activity (%) as analyzed by luciferase assay of all the constructs is indicated in […]
3. A promoter deletion study was done in order to determine the binding sites for a transcription factor on the promoter, which is activated on treatment with the drug ‘X’. The following constructs were made- The following statements can be made. A. Region between -1800 and -1210 contains a binding site for the activator. B. […]
Which of the following methods can be used to study protein-protein interactions? (1) Co-immunoprecipitation (2) Peptide mapping (3) Radiolabeling (4) Enzyme linked immunoassay Introduction Protein-protein interactions (PPIs) are fundamental to virtually all biological processes, including signal transduction, cellular communication, and metabolic pathways. Studying these interactions helps us understand protein function and cellular mechanisms. Among various […]
