Key Points About ΦX174 Replication 88. Reproduction of Φ x 174, a single stranded DNA phage involves several steps. A few statements are given below to explain the mechanism. […]
Tag: DNA REPLICATION CSIR NET PYQ
Tag: DNA REPLICATION CSIR NET PYQ
DNA REPLICATION CSIR NET PYQ
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Key Facts About Mitochondria: D-loop Function, Strand Composition, tRNA Import, Protein Synthesis Inhibitors, and mtDNA Forms
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- June 14, 2025
- 25 Comments
Following statements were made about mitochondria: (A) The D loop of the mitochondrial genome is required for replication, but not for the regulation of transcription. (B) The L strand of […]
Key Features of the Human Mitochondrial Genome: Replication, Transcription, and Structure
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- June 14, 2025
- 14 Comments
86. Following statements were made about human mitochondrial genome: A. The replication of both the H and L strands is unidirectional and begins at specific origins B. Majority of the […]
Understanding the High Copy Number of pUC18: Mutation in RNAII and Absence of Rop Gene
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- June 14, 2025
- 6 Comments
The ColE1 plasmid has a low to medium copy number. However, pUC18, which is also a ColE1- based plasmid, has a high copy number because: (1) It has a mutation […]
How Mutations in RepA Dimerization Domain Convert Stringent Plasmid Replication to Relaxed Mode
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- June 14, 2025
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Plasmid copy number achieved by plasmid-encoded control elements that regulate the initiation of the replication step. For example in stringent plasmid protein Rep A dimerize and binds to origin of […]
Retroviral Reverse Transcriptase: The RNA-Dependent DNA Polymerase Essential for Viral Replication
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- June 14, 2025
- 42 Comments
Reverse transcriptase of retero virus is- (1) DNA dependent DNA polymerase (2) DNA dependent RNA polymerase (3) RNA dependent DNA polymerase (4) RNA dependent RNA polymerase What Is Retroviral […]
Understanding the Ribonuclease Activity of Reverse Transcriptase: Degradation of RNA Strand in RNA/DNA Hybrids
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- June 14, 2025
- 41 Comments
Reverse transcriptase has both ribonuclease and polymerase activities. Ribonuclease activity is required for (1)the synthesis of new RNA strand (2)the degradation RNA strand (3)the synthesis of new DNA strand (4)the […]
Insights into Plasmid Curing and Segregation: Random Distribution vs Active Partitioning in Bacteria
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- June 14, 2025
- 10 Comments
81. A bacterial population has a plasmid with copy number ‘n’. It was observed that on an average in one out of 2(n-1) cell divisions. there was spontaneous plasmid curing. […]
How RNase H Influences the Mobility of Newly Synthesized DNA Fragments in Urea-PAGE Analysis
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- June 14, 2025
- 27 Comments
In an in vitro experiment using radio-labeled nucleotides, a researcher is trying to analyze the possible products or DNA replication by resolving the products using urea-polyacrylamide gel electrophoresis. In one […]
Understanding DNA Supercoiling Removal During Replication: Roles of Helicase and Topoisomerases
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- June 14, 2025
- 29 Comments
Following statements have been made about removal of supercoiling produced by DNA unwinding at the replication fork: Accumulation of supercoils is the result of DNA helicase activity during unwinding of […]


