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45. After how many cycles of polymerase chain reaction (PCR), the amplified product of required length is generated for the first time from a chromosomal DNA template? (A) 1 (B) […]

44. A circular plasmid with restriction sites is completely digested with EcoRI and XhoI. The number of bands visible after agarose gel electrophoresis is __________. Number of Bands After Complete […]

43. The amount of agarose required to prepare 250 mL of 0.8% agarose gel is __________ grams (rounded off to the nearest integer). How Much Agarose Is Required to Prepare […]

42. Which of the given statement(s) about synthetic oligonucleotides is/are correct? (A) Chemical synthesis extends the DNA chain from 3′→5′ end (B) They can be utilized for site-directed mutagenesis (C) […]

41. Which of the following is/are essential feature(s) of high-fidelity DNA polymerases used in polymerase chain reaction? (A) 5′→3′ exonuclease activity (B) Endonuclease activity (C) 3′→5′ exonuclease activity (D) Optimum […]

40. Match the following Group I Group II P) Tissue plasminogen activator 1. Emergency treatment of heart attack Q) Erythropoietin 2. Treatment of anemia R) Superoxide dismutase 3. Prevents tissue […]

39. In the ‘Southern blot’ technique, which of the following reagents is used to detect the presence of a desired DNA fragment? (A) Ethidium bromide (B) DNA probe (C) Silver […]

38. Restriction enzymes that recognize the same nucleotide sequence but cleave at different positions are called (A) heterohypekomers (B) isocaudomers (C) isoschizomers (D) neoschizomers Restriction Enzymes Recognizing the Same Nucleotide […]

37. Transfer of plasmids into animal cells can be achieved by (A) Electroporation (B) Liposome-mediated process (C) Calcium chloride treatment (D) Sucrose treatment Transfer of Plasmids into Animal Cells: Which […]

36. While performing a PCR, the student forgot to add one of the two primers. The number of molecules of single-stranded DNA produced after 25 PCR cycles is ____. PCR […]