45. After how many cycles of polymerase chain reaction (PCR), the amplified product of required length is generated for the first time from a chromosomal DNA template? (A) 1 (B) […]
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IIT JAM Biotechnology Previous Year Question paper Topic Biotechniques
Complete Digestion of a Circular Plasmid
44. A circular plasmid with restriction sites is completely digested with EcoRI and XhoI. The number of bands visible after agarose gel electrophoresis is __________. Number of Bands After Complete […]
IIT JAM Biotechnology Previous Year Question paper Topic Biotechniques
How Much Agarose Is Required to Prepare 250 mL
43. The amount of agarose required to prepare 250 mL of 0.8% agarose gel is __________ grams (rounded off to the nearest integer). How Much Agarose Is Required to Prepare […]
IIT JAM Biotechnology Previous Year Question paper Topic Biotechniques
Synthetic Oligonucleotides
42. Which of the given statement(s) about synthetic oligonucleotides is/are correct? (A) Chemical synthesis extends the DNA chain from 3′→5′ end (B) They can be utilized for site-directed mutagenesis (C) […]
IIT JAM Biotechnology Previous Year Question paper Topic Biotechniques
Essential Features of High-Fidelity DNA Polymerases
41. Which of the following is/are essential feature(s) of high-fidelity DNA polymerases used in polymerase chain reaction? (A) 5′→3′ exonuclease activity (B) Endonuclease activity (C) 3′→5′ exonuclease activity (D) Optimum […]
IIT JAM Biotechnology Previous Year Question paper Topic Biotechniques
Tissue Plasminogen Activator, Erythropoietin Matching Explained
40. Match the following Group I Group II P) Tissue plasminogen activator 1. Emergency treatment of heart attack Q) Erythropoietin 2. Treatment of anemia R) Superoxide dismutase 3. Prevents tissue […]
IIT JAM Biotechnology Previous Year Question paper Topic Biotechniques
Which Reagent Is Used to Detect a Desired DNA Fragment
39. In the ‘Southern blot’ technique, which of the following reagents is used to detect the presence of a desired DNA fragment? (A) Ethidium bromide (B) DNA probe (C) Silver […]
IIT JAM Biotechnology Previous Year Question paper Topic Biotechniques
Restriction Enzymes Recognizing the Same Sequence
38. Restriction enzymes that recognize the same nucleotide sequence but cleave at different positions are called (A) heterohypekomers (B) isocaudomers (C) isoschizomers (D) neoschizomers Restriction Enzymes Recognizing the Same Nucleotide […]
IIT JAM Biotechnology Previous Year Question paper Topic Biotechniques
Transfer of Plasmids into Animal Cells
37. Transfer of plasmids into animal cells can be achieved by (A) Electroporation (B) Liposome-mediated process (C) Calcium chloride treatment (D) Sucrose treatment Transfer of Plasmids into Animal Cells: Which […]
IIT JAM Biotechnology Previous Year Question paper Topic Biotechniques
PCR with One Missing Primer
36. While performing a PCR, the student forgot to add one of the two primers. The number of molecules of single-stranded DNA produced after 25 PCR cycles is ____. PCR […]
