Tag: IIT JAM Biotechnology Previous Year Question paper Topic Wise

55. A 9.2 kb plasmid DNA shown below has four BamHI sites (p), (q), (r), (s). If a mutation results in the loss of BamHI site (r), assuming complete digestion […]

54. The nucleotide sequence of one of the DNA strands is: GCTGTAACGATAGCACGC The GC content (in %) of the double stranded DNA will be ________. How to Calculate GC Content […]

53. Which of the following methods is/are used for local alignment of nucleotide sequences? (A) Smith-Waterman algorithm (B) Needleman-Wunsch algorithm (C) BLAST (D) Neighbour-joining method Correct Answer Correct Options: (A) […]

52. Which one of the following vector types is suitable to clone 2000 kb DNA insert? (A) Bacterial plasmid (B) Bacteriophage (C) Cosmid (D) Yeast artificial chromosome Which Vector Is […]

51. Choose the option that lists the correct sequence of steps involved in gene therapy. P. Injection of expression vector into patient Q. Wild-type gene is inserted into expression vector […]

50. A linear DNA contains five restriction sites for EcoRI and three restriction sites for BamHl. The number of fragments that will be generated after digestion with EcoRI is      […]

49. The total number of DNA molecules present after 5 cycles of polymerase chain reaction (PCR) starting with 3 molecules of template DNA is        . How Many DNA Molecules […]

48. A schematic representation of restriction fragment length polymorphism (RFLP) analysis of a sample population is shown below. The number of people exhibiting a given pattern is indicated above the […]

47. The vector, shown in the figure, has promoter and RBS sequences in the 300 bp region between the restriction sites for enzymes X and Y. There are no other […]

46. Bacterial cloning vectors and bacterial expression vectors are differentiated by the presence of (A) antibiotic resistance gene cassette (B) origin of replication (C) promoter and ribosome-binding site (D) unique […]