Category: GATE Biotechnology 2023 Previous Year Papers with Solutions

Category: GATE Biotechnology 2023 Previous Year Papers with Solutions

GATE Biotechnology 2023 Previous Year Papers with Solutions

Q.65 A dNTP master-mix is prepared by combining 40 μL of each 20 mM dNTP stock (dATP, dCTP, dGTP and dTTP). 4 μL of this dNTP master-mix is added to a PCR mix and the final volume is adjusted to 50 μL. The concentration (in μM) of total dNTPs in the PCR mix is ____________.

dNTP Master-Mix PCR Concentration Calculation

Q.64 A proportional controller is used to control the temperature of an autoclave from 60 °C to 130 °C. If the proportional band setting of the controller is 25%, the proportional gain value is ____________.

Proportional Gain Calculation

Q.63 An evaporator is insulated using glass wool material of 0.15 m thickness. The inner most surface and the outer surface of the insulation are at 700 °C and 80 °C, respectively. The mean thermal conductivity of the glass wool under these conditions is 0.29 W.m−1.K−1. The rate of heat loss (in W) through 1.2 m2 of the evaporator wall surface (rounded off to the nearest integer) is ____________.

Evaporator Heat Loss Calculation

Q.62 The values of the consistency index ‘K’ and the flow behavior index ‘n’ of a dilatant fluid are 0.415 (in CGS units) and 1.23, respectively. The value of the apparent viscosity (in g.cm−1.s−1) of this fluid at a shear rate of 60 s−1 (rounded off to the nearest integer) is ____________.

Apparent Viscosity of Dilatant Fluid

Q.61 Ten playing cards numbered 1, 2, 3, ...., 10 are placed face down on a table. One card is drawn at random, its number recorded, and then replaced face down. A card is drawn again at random. The probability that the number on the second draw is greater than the number on the first draw (rounded off to two decimal places) is ___________.

Probability Second Card Greater Than First

Q.60 If f(2) = 5 and (f(x))(f(x+1)) = 3 for all real values of x, the value of f(10) is ____________.

Solving f(2)=5, f(x)f(x+1)=3

Q.59 If f(x) = (sin x + cos x) / (sin x − cos x), the value of f′(x) at x = 0 is ____________.

Derivative of (sin x + cos x)/(sin x – cos x) at x=0

Value of |A⁴ + 3A² − 5A + 6I| for A = (1 2; 3 5)

Q.57 A protein has three identical sites arranged at the vertices of an equilateral triangle. If one site is filled with a dye (donor), the measured quantum yield (ϕD) is 0.5. Filling one site with a donor dye and a second site with an acceptor dye results in ϕD of 0.25. The measured ϕD of one site filled with donor and the other two sites filled with acceptor dye (rounded off to three decimal places) is ____________.

Protein Quantum Yield FRET

Q.56 If 1000 bp of a double-helical DNA weighs 1×10−18 gm and distance between two bp is 0.34 nm, the total amount of DNA (in mg, rounded off to one decimal place) required to stretch from Earth to Moon (assuming the distance between Earth and Moon to be 3,74,000 km) is ____________.

Total DNA Amount to Stretch from Earth to Moon

Q.55 DNA sample collected from an unidentified bacterial species (Y) contains 13% of adenine. The G+C content (in percentage) of Y is ___________.

DNA Adenine 13% G+C Content Bacterial Species Calculation

Q.54 An enzyme (E) catalyzes the biochemical reaction A → B with kcat equal to 500 s−1. If the initial reaction velocity (V0) is 10 μM.s−1 at the total enzyme concentration [Et] of 30 nM and substrate concentration [A] of 40 μM, the value of Km (in μM) is ____________.

Enzyme Km Calculation

Q.53 The following schematic diagram shows a chemostat with cell recycle Where F0 and Fr are the volumetric flow rates (in L.h−1) of feed and recycle streams, respectively. X1, X0 and X are the cell concentrations (in g.L−1) in the reactor, recycle-stream and product-stream, respectively. If (X0 / X1) = 1.5, (Fr / F0) = 0.7 and X1 is 7.3 g.L−1, the value of X (in g.L−1, rounded off to one decimal place) is ____________.

Chemostat With Cell Recycle

Q.52 The number of different possible ways of forming five intramolecular disulfide bonds with ten cysteine residues of a protein is ___________.

Number of Ways to Form Five Intramolecular Disulfide Bonds

Q.51 If there are three unrooted trees for four protein sequences, the number of rooted trees for the same number of sequences is ___________.

Number of Rooted Trees for Four Protein Sequences

Q.50 E. coli cultivated at 298 K uptakes an uncharged compound (A) by passive diffusion. The intracellular and extracellular concentrations of A are 0.001 M and 0.1 M, respectively. If the value of the ideal gas constant R is 1.9872 cal.mol−1.K−1, the free-energy change (in kcal.mol−1) for this passive diffusion of A (rounded off to two decimal places) is ____________.

Free Energy Change Passive Diffusion E. coli

Q.49 Aqueous two-phase extraction is used to recover α-amylase from a solution. A polypropylene glycol-dextran mixture is added and the solution separates into upper and lower phases. The partition coefficient is 4.0 and the ratio of upper to lower phase volume is 5.0. The enzyme recovery or yield (in percentage, rounded off to the nearest integer) is ___________.

Aqueous Two-Phase Extraction α-Amylase Recovery

Q.48 E. coli is cultivated in a chemostat operated at a dilution rate of 0.2 h−1. The values of biomass yield due to oxygen consumption and the steady state biomass concentration are 0.2 g.g−1 and 10 g.L−1, respectively. The oxygen transfer rate (in g.L−1.h−1) is ____________.

Oxygen Transfer Rate Calculation in Chemostat: E. coli Cultivation

Q.47 Temperature of a reaction with an activation energy value of 15 kcal.mol−1 is increased from 300 K to 310 K. If the value of the ideal gas constant (R) is 1.9872 cal.mol−1.K−1, the ratio of the reaction rate constants (k310/k300) (rounded off to two decimal places) is ____________.

Ratio of Reaction Rate Constants

Q.46 The following reaction represents biomass synthesis from hexadecane C16H34 + 12.5O2 + 2.13NH3 → 10.6CH1.66O0.27N0.27 + 5.37CO2 + 11.4H2O where CH1.66O0.27N0.27 represents the biomass. The value of respiratory quotient (rounded off to two decimal places) is ____________.

Respiratory Quotient in Biomass Synthesis from Hexadecane

Q.45 Which of the following vector(s) is(are) used to clone a DNA fragment of size 220 kb? (A) Bacterial artificial chromosome (B) Yeast artificial chromosome (C) Cosmids (D) pUC19 plasmid

Which Vectors Clone 220 kb DNA Fragments?

Q.44 Direct DNA transfer method(s) used for plant genetic engineering is(are) (A) microparticle bombardment (B) electroporation (C) polyethylene glycol treatment (D) Agrobacterium-mediated transformation

Direct DNA Transfer Methods in Plant Genetic Engineering

Q.43 Which of the following is(are) plant protoplast fusogenic agent(s)? (A) Sodium nitrate (B) Polyvinyl alcohol (C) Polyethylene glycol (D) Bromoxynil

Plant Protoplast Fusogenic Agents

Q.42 Which of the following statement(s) is(are) TRUE about fluoroquinolone drugs? (A) They contain quinolone ring(s) (B) They inhibit RNA polymerase (C) They bind to bacterial topoisomerase (D) They bind to 23S rRNA within the 50S ribosome subunit

Fluoroquinolone Drugs

Q.41 Which of the following statement(s) is(are) TRUE about induced pluripotent stem cells? (A) They can self-renew (B) They require specific signals to maintain their stemness (C) They cannot be genetically manipulated (D) They can form organoids in vitro

Induced Pluripotent Stem Cells

Q.40 Methylation of CpG islands near the promoter of a gene can inhibit transcription by (A) preventing RNA polymerase binding (B) facilitating repressor binding (C) facilitating heterochromatin formation (D) inducing euchromatin formation

Methylation of CpG Islands Near Promoter

Q.39 The event(s) that lead(s) to inactivation of tumor suppressor genes in cancer cells is(are) (A) gene amplification (B) promoter methylation (C) loss of heterozygosity (D) histone acetylation

Tumor Suppressor Genes Inactivation

Q.38 Match the virus in Group I with the type of genome it contains in Group II. Group I                                                  Group II P. T4 bacteriophage                            1. dsRNA Q. SARS-CoV-2                                    2. ssDNA R. Pseudomonas phage Φ6               3. dsDNA S. ΦX174 bacteriophage                    4. ssRNA (A) P-3, Q-4, R-1, S-2 (B) P-2, Q-1, R-4, S-3 (C) P-4, Q-3, R-2, S-1 (D) P-1, Q-4, R-2, S-3

T4 Bacteriophage SARS-CoV-2 Pseudomonas Phage Φ6 ΦX174 Genome Matching

Q.37 Match the type of bacteria in Group I with their respective growth properties in Group II. Group I                                        Group II P. Halophile                     1. Grows optimally between 20 °C and 45 °C Q. Piezophile                    2. Grows best at low water activity R. Mesophile                    3. Grows at high level of salt S. Xerophile                      4. Grows optimally at high hydrostatic pressure (A) P-3, Q-4, R-1, S-2 (B) P-2, Q-3, R-4, S-1 (C) P-3, Q-1, R-2, S-4 (D) P-4, Q-3, R-1, S-2

Matching Halophile, Piezophile, Mesophile, Xerophile Bacteria Growth Properties

Q.36 Match the immune tolerance mechanisms in Group I with their respective outcomes in Group II. Group I                                                                            Group II P. Anergy                                                           1. Elimination of activated T-cells after antigen clearance Q. Activation-induced cell death                  2. Inhibition of auto-reactive T-cells at periphery R. Receptor editing                                          3. Unresponsiveness to antigens due to lack of co-stimulatory molecules S. Regulatory T-cells                                        4. Elimination of auto-reactive B-cells (A) P-3, Q-1, R-4, S-2 (B) P-4, Q-3, R-1, S-2 (C) P-3, Q-4, R-2, S-1 (D) P-3, Q-2, R-4, S-1

Immune Tolerance Mechanisms Matching

Q.35 The distance between the two points of intersection of x² + y = 7 and x + y = 7 (rounded off to two decimal places) is ____________.

Distance Between Points of Intersection

Q.34 If 73x = 216 , the value of 7−x (rounded off to three decimal places) is ____________.

Rounded to Three Decimal Places

Q.33 Two fair six-sided dice are thrown. The probability of getting 12 as the product of the numbers on the dice (rounded off to two decimal places) is ____________.

Probability of Getting 12 as Product of Two Dice

Q.32 A series (S) is given as S = 1 + 3 + 5 + 7 + 9 + …… The sum of the first 50 terms of S is ____________.

Sum of First 50 Terms of 1 + 3 + 5 + 7 + 9 Series

Q.31 The value of limx→0[(cos 2x − cos 4x) / x²] is ____________.

Lim x→0 (cos 2x – cos 4x)/x² Value and Solution

Q.30 Fabry disease in humans is a X-linked disease. The probability (in percentage) for a phenotypically normal father and a carrier mother to have a son with Fabry disease is ____________.

 Fabry Disease X-Linked Inheritance

Q.29 Which one of the following plant growth regulators facilitate adventitious root formation? (A) Auxin (B) Zeatin (C) Dihydrozeatin (D) Kinetin

Auxin Facilitates Adventitious Root Formation

Q.28 Which one of the following methods CANNOT be used to determine the secondary structure content of a protein? (A) Circular dichroism spectroscopy (B) Fourier transform infrared spectroscopy (C) Mass spectrometry (D) X-ray crystallography

Which Method Cannot Determine Protein Secondary Structure Content?

Q.27 Which one of the following is used for global alignment of two protein sequences? (A) Chou-Fasman method (B) Garnier-Osguthorpe-Robson (GOR) method (C) Needleman-Wunsch algorithm (D) Smith-Waterman algorithm

Needleman-Wunsch Algorithm

Q.26 Which one of the following programs is used for finding distantly related (or remote) protein homologs? (A) BLASTN (B) BLASTX (C) PSI-BLAST (D) TBLASTX

PSI-BLAST for Finding Distantly Related Protein Homologs

Q.25 Match the types of RNA in Group I with their corresponding function in Group II. Group I                                       Group II P. mRNA                       1. Serves as adaptors between mRNA and amino acids during protein synthesis Q. rRNA                        2. Regulates post-transcriptional gene expression R. miRNA                     3. Codes for proteins S. tRNA                         4. Forms the core of the ribosome structure and catalyzes protein synthesis (A) P-3, Q-4, R-2, S-1 (B) P-3, Q-4, R-1, S-2 (C) P-4, Q-3, R-2, S-1 (D) P-2, Q-1, R-4, S-3

Types of RNA Matching

Q.24 Which of the following methods are used for detection of DNA and RNA, respectively? (A) Southern and Northern blotting (B) Southern and Western blotting (C) Northern and Southern blotting (D) Northern and Western blotting

DNA and RNA Detection Methods

Q.23 In hybridoma technology, which one of the following enzymes is absent in the myeloma cells that are used for monoclonal antibody production? (A) Hypoxanthine-guanine phosphoribosyltransferase (B) Alanine aminotransferase (C) Triose phosphate isomerase (D) Glycosyltransferase

Hybridoma Technology

Q.22 In ELISA, which of the following enzymes are conjugated to antibodies for detection of the analyte? P. Alkaline phosphatase Q. Trypsinase R. Horseradish peroxidase S. Amylase (A) P and R (B) P and Q (C) Q and S (D) R and S

 ELISA Enzymes

Q.21 Intracellular proteins are targeted for proteolytic degradation in proteasomes upon conjugation with (A) ubiquitin (B) integrin (C) peptidase (D) calreticulin

Intracellular Proteins Targeted

Q.20 The correct sequence of events during bacteriophage infection of a bacterial cell is (A) landing → attachment → tail contraction → penetration and unplugging → DNA ejection (B) attachment → landing → penetration and unplugging → tail contraction → DNA ejection (C) landing → tail contraction → attachment → DNA ejection → penetration and unplugging (D) attachment → tail contraction → landing → penetration and unplugging → DNA ejection

Bacteriophage Infection Sequence

Q.19 Which one of the following statements is TRUE about leghemoglobin? (A) It binds oxygen to protect nitrogenase (B) It binds hemoglobin to protect oxygenase (C) It binds oxygen to protect hydrogenase (D) It binds oxygen to protect oxygenase

Leghemoglobin Function

Q.18 Which one of the following is required for the development of B-cells in the bone marrow? (A) Stromal cells (B) Dendritic cells (C) Kupffer cells (D) NK cells

B-Cell Development in Bone Marrow

Q.17 Dendritic cells are involved in cross-presentation of antigens. Which of the following protein(s) is(are) required for cross-presentation? P. Basic leucine zipper ATF-like transcription factor 3 (BATF3) Q. Membrane associated ring-CH-type finger 1 (MARCH-1) R. Solute carrier family 10 member 1 (SLC10A1) S. Class II-associated invariant chain peptide (CLIP) (A) P only (B) P and R only (C) P, Q and R only (D) S only

BATF3 in Dendritic Cell Cross-Presentation

Q.16 Which one of the following drugs is NOT an immune checkpoint inhibitor? (A) Ipilimumab (B) Pembrolizumab (C) Nivolumab (D) Trastuzumab

Which Drug is NOT an Immune Checkpoint Inhibitor?

Q.15 C-value paradox refers to (A) the lack of correlation between genome size and genetic complexity of an organism (B) the presence of genetic sequences that propagate themselves within a genome (C) the coexistence of multiple alleles at a genetic locus (D) the concept that two or more genes may have the same function

C-Value Paradox Explained

Q.14 Determine the correctness or otherwise of the following Assertion [a] and the Reason [r]. Assertion [a]: Chromosome mutations can change the structure of chromosomes. Reason [r]: All chromosome mutations arise due to nondisjunction of chromosomes during mitosis or meiosis. (A) Both [a] and [r] are false (B) [a] is true but [r] is false (C) Both [a] and [r] are true and [r] is the correct reason for [a] (D) Both [a] and [r] are true but [r] is not the correct reason for [a]

Chromosome Mutations Assertion-Reason

Q.13 Determine the correctness or otherwise of the following Assertion [a] and the Reason [r]. Assertion [a]: In multicellular organisms, cells of different lineages have different gene expression profiles. Reason [r]: Alternative splicing is the only mechanism to generate protein diversity. (A) Both [a] and [r] are false (B) Both [a] and [r] are true and [r] is the correct reason for [a] (C) Both [a] and [r] are true but [r] is not the correct reason for [a] (D) [a] is true but [r] is false

Multicellular Organisms Gene Expression Profiles

Q.12 Acetylcholine released by the parasympathetic nerves has which one of the following functions in the heart pacemaker cells? (A) It binds to GPCR and activates G protein to slow the heart rate (B) It stimulates GABA-activated ion-channel coupled receptor to increase the heart rate (C) It binds to GPCR and inhibits G protein to slow the heart rate (D) It inhibits GABA-activated ion-channel coupled receptor to increase the heart rate

Acetylcholine Parasympathetic Nerves Heart Pacemaker Cells

Q.11 Eukaryotic transcription is carried out by (A) DNA-dependent RNA polymerase (B) DNA-dependent DNA polymerase (C) RNA-dependent DNA polymerase (D) RNA-dependent RNA polymerase

Eukaryotic Transcription by DNA-Dependent RNA Polymerase

Q.10 Which one of the following shapes can be used to tile (completely cover by repeating) a flat plane, extending to infinity in all directions, without leaving any empty spaces in between them? The copies of the shape used to tile are identical and are not allowed to overlap. (A) circle (B) regular octagon (C) regular pentagon (D) rhombus

Which Shape Tiles a Plane Without Gaps

Q.9 The coefficient of x4 in the polynomial (x − 1)3(x − 2)3 is equal to ______. 33 −3 30 21

Coefficient of x^4 in (x-1)^3(x-2)^3

Q.8 The World Bank has declared that it does not plan to offer new financing to Sri Lanka, which is battling its worst economic crisis in decades, until the country has an adequate macroeconomic policy framework in place. In a statement, the World Bank said Sri Lanka needed to adopt structural reforms that focus on economic stabilisation and tackle the root causes of its crisis. The latter has starved it of foreign exchange and led to shortages of food, fuel, and medicines. The bank is repurposing resources under existing loans to help alleviate shortages of essential items such as medicine, cooking gas, fertiliser, meals for children, and cash for vulnerable households. Based only on the above passage, which one of the following statements can be inferred with certainty? (A) According to the World Bank, the root cause of Sri Lanka’s economic crisis is that it does not have enough foreign exchange. (B) The World Bank has stated that it will advise the Sri Lankan government about how to tackle the root causes of its economic crisis. (C) According to the World Bank, Sri Lanka does not yet have an adequate macroeconomic policy framework. (D) The World Bank has stated that it will provide Sri Lanka with additional funds for essentials such as food, fuel, and medicines.

World Bank Sri Lanka Economic Crisis

Q.7 To construct a wall, sand and cement are mixed in the ratio of 3:1. The cost of sand and that of cement are in the ratio of 1:2. If the total cost of sand and cement to construct the wall is 1000 rupees, then what is the cost (in rupees) of cement used? (A) 400 (B) 600 (C) 800 (D) 200

Sand Cement Ratio 3:1 Cost 1:2 Total 1000 Rupees

Q.6 Human beings are one among many creatures that inhabit an imagined world. In this imagined world, some creatures are cruel. If in this imagined world, it is given that the statement “Some human beings are not cruel creatures” is FALSE, then which of the following set of statement(s) can be logically inferred with certainty? (i) All human beings are cruel creatures. (ii) Some human beings are cruel creatures. (iii) Some creatures that are cruel are human beings. (iv) No human beings are cruel creatures. (A) only (i) (B) only (iii) and (iv) (C) only (i) and (ii) (D) (i), (ii) and (iii)

Logical Inference When “Some Humans Are Not Cruel” Is False

Q.5 A line of symmetry is defined as a line that divides a figure into two parts in a way such that each part is a mirror image of the other part about that line. The given figure consists of 16 unit squares arranged as shown. In addition to the three black squares, what is the minimum number of squares that must be coloured black, such that both PQ and MN form lines of symmetry? (The figure is representative) (A) 3 (B) 4 (C) 5 (D) 6

Minimum Squares to Color Black

Q.4 In 2022, June Huh was awarded the Fields medal, which is the highest prize in Mathematics. When he was younger, he was also a poet. He did not win any medals in the International Mathematics Olympiads. He dropped out of college. Based only on the above information, which one of the following statements can be logically inferred with certainty? (A) Every Fields medalist has won a medal in an International Mathematics Olympiad. (B) Everyone who has dropped out of college has won the Fields medal. (C) All Fields medalists are part-time poets. (D) Some Fields medalists have dropped out of college.

Fields Medalist June Huh

Q.3 In the given figure, PQRS is a parallelogram with PS = 7 cm, PT = 4 cm and PV = 5 cm. What is the length of RS in cm? (The diagram is representative.) (A) 20/7 (B) 28/5 (C) 9/2 (D) 35/4

PQRS Parallelogram Solved

Q.2 References : ______ : : Guidelines : Implement (By word meaning) (A) Sight (B) Site (C) Cite (D) Plagiarise

References Cite Guidelines Implement Analogy

Q.1 “You are delaying the completion of the task. Send _______ contributions at the earliest.” (A) you are (B) your (C) you’re (D) yore

Delay Task Completion?

Latest Courses

IIT JAM / CUET- PG

IIT JAM / CUET- PG

Are you aspiring to crack the IIT JAM Biotechnology exam with flying colors? Choosing the right coaching institute is crucial for your success. Let's Talk Academy (LTA) has established itself as the best coaching institute for IIT JAM Biotechnology, helping students achieve their dreams through expert guidance, structured study material, and personalized mentorship.

CSIR UGC NET Life Science Course

CSIR UGC NET Life Science Course

Let's Talk Academy is the number one CSIR NET Life Science coaching in Jaipur for Life Science. At Let's Talk Academy, you can be sure to get the right coaching strictly in adherence to the CSIR NET Life Science course.  CSIR NET Life Science is an exam that requires in-depth knowledge, consistent practice, and the right guidance to crack. If you are looking for a place where you can get the best CSIR NET Life Science coaching, you are at the right place.

ICMR JRF Life Science

ICMR JRF Life Science

If you are serious about cracking the ICMR Life Science JRF exam, Let’s Talk Academy is your ultimate destination. Join now and take the first step toward a successful research career!