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9. Which of the following statements about HPLC is not correct? A. There are two phases to HPLC: mobile and stationary. B. Separation, identification, and purification of proteins are just […]

8. Which of the following peptides elutes first in size-exclusion chromatography? If a mixture of cytochrome-c, proteinase inhibitor, helicase, serum albumin and RNA polymerase with molecular weights 13.7, 23, 68, […]

7. Mark the correct sequence in which myoglobin (16 kDa), catalase (221 kDa), cytochrome c (13 kDa), myosin (524 kDa), and serum albumin (68 kDa) are eluted from a molecular-exclusion […]

6. What type of chromatography can be used to isolate mRNA using a column of oligo (dT)-cellulose? A. Partition chromatography B. Ion-exchange chromatography C. Affinity chromatography D. Adsorption chromatography Oligo(dT)-cellulose […]

5. Two proteins having a slight difference in size are subjected to size-exclusion chromatography and partially flow from each other. If longer columns show no improvement in separation, it is […]

4. Protein A and protein B have the same molecular weight and isoelectric point but differ in amino acid composition. Which of the following techniques is best to separate them? […]

3. Proteins A, B and C have decreasing average hydrophobicity respectively and the same molecular mass but different amino acid compositions. If the mixture is subjected to reverse-phase chromatography using […]

2. Liquid Chromatography Mass Spectrometry (LCMS) is characterized by: A. Allows fast separation and analysis of proteins B. Separates proteins from metals C. Separates trypsin from peptides D. None of […]

1. How can two proteins be purified at physiological pH if both have the same mass but the pI of one is 6.5 and different from the other? A. Ion-exchange […]