13. A merodiploid strain of E. coli with the genotype F+OCZ–Y+/ O+Z+Y+was constructed. The activity of β-galactosidase enzyme was measured in this strain upon following treatments. (A) no induction (B) […]
Tag: operon pyq
Tag: operon pyq
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Catabolite Repression in Bacteria
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- June 11, 2025
- 20 Comments
A bacterial stra•n can use carbohydrates and hydrocarbons as growth substrates. The strain uses glucose following a minimal lag period after inoculation, regardless of the other carbohydrates and hydrocarbons in […]
Budding Yeast Show Two Lag Phases When Grown on High Glucose
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- June 11, 2025
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11. When budding yeast (a facultative anaerobe) is grown for a few days in medium containing high glucose it shows a growth pattern with two lag phases (see figure below). […]
The Role of Lactose Permease and the Lac Operon
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- June 11, 2025
- 21 Comments
10 Two E.coli Culture A and B are taken. Culture A was earlier grown in the presence of optimum concentration of gratuitous inducer IPTG. Both the cultures are now used […]
Diauxic Growth in E. coli: How Glucose and Lactose Shape the Bacterial Growth Curve
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- June 11, 2025
- 22 Comments
9. A culture medium contains two carbon sources, one is preferred carbon source (glucose) and the second is a non-preferred source (lactose). Which one below is correct regarding the nature […]
Glucose Addition and Lac Operon Expression
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- June 11, 2025
- 26 Comments
8. E. coli cells are growing in a medium containing lactose but no glucose. Indicate the expression status of the lac operon if glucose is added to the culture. (1) […]
How β-Galactosidase Expression in E. coli Changes After Glucose Depletion in a Galactose Medium
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- June 11, 2025
- 25 Comments
7. E. coli is being grown in a medium containing both glucose and galactose. On depletion of glucose, expression of β-galactosidase will (1) remain unchanged (2) increase (3) decrease (4) […]
Lac Operon Regulation By High cAMP, Allolactose and CAP Activity
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- June 11, 2025
- 25 Comments
6. The lac operon in E. coli. is controlled by both the lac repressor and the catabolite activation protein CAP. In an in vivo experiment with lac operon, the following […]
E. coli Lac Operon Both Positively and Negatively Regulated
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- June 11, 2025
- 22 Comments
In E. coli lac operon is positively as well as negatively regulated. This suggest that (1) E. coli rely only on glucose for energy (2) E. coli rely only on […]
Glucose and Lactose Regulation in the Lac Operon in E. coli
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- June 11, 2025
- 27 Comments
Which one of the following conditions will switch on Lac operon in E. coli? (1) + Glucose, + Lactose (2) + Glucose, – Lactose (3) – Glucose, – Lactos (4) […]


