Interpreting Hemoglobin Electrophoresis and Western Blot Results for Sickle Cell Disease You order a hemoglobin electrophoresis on a patient suspected of having sickle cell disease. A blood sample was obtained […]
Tag: csir previous year question
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CSIR NET Previous Year Questions on Blotting
Best Technique to Study Protein Half-Life in Animal Cells
Best Technique to Study Protein Half-Life in Animal Cells Which of the following techniques should be used to study the half-life of protein A in an animal cell that can […]
CSIR NET Previous Year Questions on Blotting
Limitations of Northern Blotting
Limitations of Northern Blotting or Which of the following analysis cant be done using Northern blotting A. Approximate size of an mRNA B. Relative abundance of an mRNA in various […]
CSIR NET Previous Year Questions on Blotting
Interpreting Southern Blot Results
Interpreting Southern Blot Results Isolated variety of E .coli is followed by southern blot procedure. A radio active probe is then hybridized with gene of interest & observed single band […]
CSIR NET Previous Year Questions Gel Electrophoresis
Cytochrome-C and Myoglobin separated by polyacrylamide gel electrophoresis
A mixture of Cytochrome-C (MW 11.7 kD) and Myoglobin (MW 17.2 KD) are to be separated by polyacrylamide gel electrophoresis. Their isoelectric pH (pI) values are 9.6 and 7.2 respectively. […]
CSIR NET Previous Year Questions Gel Electrophoresis
APS (Ammonium persulphate) is used in SDS-PAGE
APS (Ammonium persulphate) is used in SDS-PAGE for (A) preventing oxidation (B) cross-linking (C) its role as a catalyst (D) free radical formation Correct answer: (D) Free radical formation Explanation: […]
Education
Electrophoresis of a purified protein in SDS-PAGE in the presence of b-marcaptoethanol yields two bands
Electrophoresis of a purified protein in SDS-PAGE in the presence of b-marcaptoethanol yields two bands of 35 kDa and 45 kDa. However, in a gel filtration chromatography, the same protein […]
Education
Separated the two proteins from the mixture one is monomeric
What method we should use to separated the two proteins from the mixture in which one is monomeric with MW of 10 kDa and other is of two Homodimer’s with […]
CSIR NET Previous Year Questions Gel Electrophoresis
Chymotrypsinogen in the native state and reduced unfolded sates is loaded- Gel-electrophoresis
Chymotrypsinogen in the native state and reduced unfolded sates is loaded in well A and B respectively. They are electrophoreses and stain, one of the following results is likely to […]
CSIR NET Previous Year Questions Gel Electrophoresis
Proteins A and B having a molecular weight of 16500 and 35400 move 1.3 cm and 4.6 cm
Proteins A and B having a molecular weight of 16500 and 35400 move 1.3 cm and 4.6 cm, respectively, when electrophoresed through a gel. What is the molecular weight of […]
