Best Technique to Study Protein Half-Life in Animal Cells
Which of the following techniques should be used to study the half-life of protein A in an animal cell that can be after labelling all proteins being translated with a radioactive amino acid for a short duration followed by removal of radiolabel and growth of cell in normal medium.
A. SDS PAGE followed by autoradiography
B. SDS-PAGE followed by Western blotting
C. Immunoprecipitation followed by autoradiography
D. Immunoprecipitation followed by SDS-PAGE and autoradiography
Best Technique to Study Protein Half-Life in Animal Cells
Correct Answer: (D) Immunoprecipitation Followed by SDS-PAGE and Autoradiography
Studying the half-life of a protein requires a technique that can specifically track the decay of a labeled protein over time. In this experiment, all proteins are initially labeled using a radioactive amino acid pulse (pulse-chase experiment), followed by growing the cells in a non-radioactive medium (chase). The best method to track the degradation of Protein A is Immunoprecipitation (IP) followed by SDS-PAGE and Autoradiography.
Why is Option (D) the Best Choice to Study Protein Half-Life in Animal Cells ?
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Immunoprecipitation Ensures Protein Specificity
- Since all proteins are initially labeled, a specific purification method is required to isolate only Protein A.
- Immunoprecipitation (IP) achieves this by using antibodies specific to Protein A, allowing selective extraction from the complex cellular mixture.
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SDS-PAGE Helps Analyze Protein Size and Degradation
- After immunoprecipitation, SDS-PAGE is performed to separate the protein based on its molecular weight, enabling comparison across different time points.
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Autoradiography Detects the Labeled Protein
- Since Protein A was originally labeled with a radioactive amino acid, autoradiography can track its presence and decay over time.
- A gradual decrease in the autoradiographic signal indicates protein degradation and half-life estimation.
Why Other Options Are Less Suitable?
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(A) SDS-PAGE followed by Autoradiography:
- This method detects all labeled proteins, not just Protein A, making it non-specific.
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(B) SDS-PAGE followed by Western Blotting:
- Western blot detects total protein levels, but it cannot distinguish between newly synthesized vs. pre-existing proteins in pulse-chase experiments.
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(C) Immunoprecipitation followed by Autoradiography:
- While this isolates Protein A, without SDS-PAGE, we cannot confirm if degradation products exist.
Conclusion
To measure Protein A’s half-life, Immunoprecipitation followed by SDS-PAGE and Autoradiography is the most precise technique, ensuring specificity, molecular weight confirmation, and detection of radioactive decay over time.
51 Comments
Shreeji Charan
March 1, 2025🙋♀️
Suman bhakar
March 3, 2025Done
Arushi
March 3, 2025👍👍
Priti khandal
March 3, 2025Okk sir
Prami Masih
March 4, 2025Done sir ✅
Ekta
August 20, 2025👍👍
Varsha Tatla
August 20, 2025___
Varsha Tatla
August 20, 2025Clear with explanation
Divya rani
August 21, 2025Immunoprecipitation ….ensures the Protein specificity
Auto radiography…….detect the labelled protein
SDS page ….helps in detection of protein size and degradation.
Priya choudhary
August 20, 2025Right answer is option d
Khushi Pareek
August 21, 2025Immunoprecipitation is a technique to isolate and identify a specific protein from a complex mixture
Answer d
Khushi Vaishnav
August 21, 2025Immuno precipitation by SDS PAGE and Autoradiography
Soniya Shekhawat
August 22, 2025Protein half life is have detected by the immuno precipitation which is followed by the sds page and auto radiography.
Khushi Singh
August 22, 2025Done
Priya dhakad
August 23, 2025To measure Protein A half-life, Immunoprecipitation followed by SDS-PAGE and Autoradiography is the most precise technique.
Priya khandal
August 23, 2025Immunoprecipitation followed by sds page and autoradiography
Kanishka Parnami
August 23, 2025Done
Sumit Chhimpa
August 24, 2025Option (D) is the Best Choice to Study Protein Half-Life in Animal Cells because Immunoprecipitation is a specific purification method that is required to isolate protein or protein specificity.
Here the SDS page helps analyze protein size & degradation… With it Autoradiograpgy tracks the presence of Protein A & it’s decay over time….
Bharti Yadav
August 23, 2025For study half-life of a protein Immunoprecipitation followed by SDS-PAGE and Autoradiography is best technique.
Sonam Saini
August 24, 2025Opetion d is Right answer because half Life of protein imunopripetation flooded bye SDS, page and autoradigraphi is Best techniques
Sheikh Aleen
August 24, 2025Immunoprecipitation followed by SDS-PAGE and Autoradiography
Khushi Agarwal
August 24, 2025Option d is correct
Immunoprecipitation Followed by SDS-PAGE and Autoradiography
This is best way to study half life in animal cell
Yogita
August 24, 2025Immuno precipitation followed by SDS page and Autoradiography
Bhavana kankhedia
August 24, 2025Immunoprecipitation Followed by SDS-PAGE and Autoradiography
Aakanksha Sharma
August 24, 2025Immunoprecipitation followed by sds page & autoradiography
Himanshi
August 24, 2025Immunoprecipitation followed by SDS-PAGE and Autoradiography
Meenakshi Choudhary
August 24, 2025Immunoprecipitation followed by SDS-PAGE and autoradiography
AKANKSHA RAJPUT
August 24, 2025Option d is correct
Ayush Dubey
August 24, 2025D. Immunoprecipitation followed by SDS-PAGE and autoradiography
Tanvi Panwar
August 24, 2025Immunoprecipitation is a technique used to isolate a specific protein from a complex mixture followed by SDS PAGE which detects degradation of protein and then autoradiography.
Kajal
August 24, 2025Option d immuno ppt followed by sds page n autoradiography is correct one
Priyanka Verma
August 24, 2025option d is correct
immunoprecipitation ensures the protein specificity
autoradiography helps to label the protein and SDS size helps to detect the size of protein
MOHIT AKHAND
August 24, 2025Done sir ✅
Parul Yadav
August 24, 2025Immunoprecipitation followed by SDS-PAGE and Autoradiography are techniques to study a specific Protein “A” Half Life in animal cells
Surendra Doodi
August 24, 2025Immunoprecipitation Followed by SDS-PAGE and Autoradiography
Falguni Prakash
August 24, 2025D
Pooja Sharma
August 25, 2025OPTION D
Priyanka Meena
August 26, 2025Best technique to study protein half life in animal cells immunopreciptiation followed by SDS PAGE and autoradiography.
Manisha choudhary
August 26, 2025Immunoprecipitation technique k dwara specific antibody k through protein A ko other radiolabeled protein s separate kr denge
Then sds page s molecular weight k base p seprate krenge jo different time point p comparison k liye helpful h
Then autoradiography k thought phle s radiolabeled protein ka decay pta kr skte h signals km aane p degradation and half life pta kr skte h
Muskan Yadav
August 26, 2025Immunoprecipitation Followed by SDS-PAGE and Autoradiography
Neha Yadav
August 27, 2025Immunoprecipitation followed by SDS – PAGE and Autoradiography
Shriyanshi verma
August 30, 2025Immuno precipitation by SDS PAGE and Autoradiography
Alec
August 30, 2025Immunoprecipitation followed by SDS PAGE and audiography.
Asha Gurzzar
August 30, 2025Sds page and autoradiography, option d is correct
Pratibha Jain
August 30, 2025Correct Answer is option (D) Immunoprecipitation Followed by SDS-PAGE and Autoradiography
anjani sharma
August 30, 2025Immunoprecipitation Followed by SDS-PAGE and Autoradiography
Kirti agarwal
August 31, 2025Opt d
Anisha jakhar
August 31, 2025Correct Answer: (D) Immunoprecipitation Followed by SDS-PAGE and Autoradiography
Kajal
August 31, 2025Correct Answer is (D) Immunoprecipitation Followed by SDS-PAGE and Autoradiography
Shobha Kanwar
August 31, 2025Option D is Right answer bcoz half Life of protein immunoprecipitation flooded by SDS, page and Auto radiography is Best techniques
Deepika sheoran
November 15, 2025Option D is correct