Interpreting Hemoglobin Electrophoresis and Western Blot Results for Sickle Cell Disease
You order a hemoglobin electrophoresis on a patient suspected of having sickle cell disease. A blood sample was obtained and the red cells were isolated. Disruption of the red cells released the hemoglobin, which was run on a polyacrylamide gel. Following the electrophoresis, a Western blot was performed to locate the hemoglobin. The results of the Western blot are shown below. Which one of the following statements best represents the interpretation of the results?
A. The band marked as X refers to the wild-type hemoglobin
B. The band in lane 2 an individual with sickle cell disease.
C. A carrier of sickle cell disease is by tie band in lane 3
D. Lane 3 an individual with sickle cell Disease
Interpreting Hemoglobin Electrophoresis and Western Blot Results for Sickle Cell Disease
Hemoglobin electrophoresis is a crucial diagnostic tool for identifying hemoglobin variants, including those associated with sickle cell disease (SCD). In this test, hemoglobin (Hb) extracted from red blood cells is separated based on charge and molecular weight using polyacrylamide gel electrophoresis (PAGE), followed by Western blotting to detect specific hemoglobin forms.
Understanding the Electrophoresis Results
64 Comments
Shreeji Charan
March 1, 2025🙋♀️
Lokesh kumawat
March 1, 2025Video solution helped a lot
Nisha Meena
March 1, 2025great resource for learning
Nisha
March 1, 2025Explantion of band shifting was very clear
Suman bhakar
March 2, 2025Done
Priti khandal
March 2, 2025Done Sir 👍
Arushi
March 3, 2025👍👍
Arushi
March 3, 2025👍done
Aakanksha Sharma
August 24, 2025Band marked as X refers to the wild type hb protein
Ujjwal
March 3, 2025✔️✔️
Akshay mahawar
March 3, 2025Done👍
Prami Masih
March 4, 2025Okay sir
VIKRAM GAHLOT
March 7, 2025ok sir
Abhilasha
March 7, 2025Okay
Kabeer Narwal
March 22, 2025👍
Neelam Sharma
August 20, 2025The band marked as X refers to the wild-type hemoglobin so band uper show
Aafreen
August 20, 2025The band marked as X refers to the wild-type hemoglobin
Manisha gujar
August 20, 2025Done sir from video explanation
Manisha gujar
August 20, 2025Option a is correct
Komal Soni
August 20, 2025Band Marked as X refers to the wild type hemoglobin
Khushi Mehra
August 20, 2025Band which is X marked refer to wild type Hb.
Mohd juber Ali
August 20, 2025Option A
( x refer wild type hemoglobin )
# X-normal (globin not break ) protein negetive charge h or disease ko anode ki or lekar jayega isliye ye carrier bhi h
Varsha Tatla
August 20, 2025Answer 1st will be correct 💯
Aman Choudhary
August 20, 2025Option A is correct
X represent wild- type hemoglobin or may be carrier both
Priya choudhary
August 20, 2025Correct answer is option a
Deepika Sheoran
August 20, 2025Option A is correct answer
Because X refers to the wild type Hemoglobin so band uper show.
Santosh Saini
August 21, 2025Band marked as x refers to the wild type hemoglobin ( globin is a part of protein it breaks into amino acid , so move fast towards the positive charge these are y band and x band is unbroken globin protein move slow ) , so option 1st is right
Sakshi Kanwar
August 21, 2025Option A. Globin protein in hemoglobin break down into amino acid in condition of sickle cell anemia and rbc shape changes . Due to breaking molecular weight of globin protein will become less it moves faster towards positive electrode and bands are seen in Y ….X bands are wild type remains towards the well because of heavy molecular weight
Manisha choudhary
August 26, 2025Nice
Pura question achhe s explain kiye ho ❤️
Komal Sharma
August 22, 2025The band marked as X refers to the wild-type hemoglobin option A is correct
Komal Sharma
August 22, 2025Globin the protein portion of himoglobin broken down into amino acid which can be sent back to the bonemerro to form new erithrocyte.
Anurag Giri
August 22, 2025Ans a
The band marked as X refers to the wild-type hemoglobin
Sneha Kumawat
August 22, 2025The correct answer is d ….3 line me sickle cell disease ke individual bands dikhenge
Khushi Singh
August 22, 2025Done
Priya dhakad
August 23, 2025Video solution is very helpful . The correct answer is A – The band marked as X refers to the wild-type hemoglobin.
Kanishka Parnami
August 23, 2025Nice
Bharti Yadav
August 23, 2025The band marked as X refers to the wild-type Hb.
Sonam Saini
August 24, 2025Opetion A is right answer
Yogita
August 24, 2025The band marked as x refers to the wild type hemoglobin
Khushi Agarwal
August 24, 2025Option a is correct answer
The band marked as X refers to the wild type hb protein bcz of their high molecular weight nd sickle cells are breakdown by globin protein so it’s molecular weight is low
Ankita Pareek
August 24, 2025Band x is of wild type hb because it’s globin is not broken down if globin breaks it’s molecular weight will less and move further
Aakanksha Sharma
August 24, 2025Band X is of wild type hb protein
Bhavana kankhedia
August 24, 2025Ans a
The band marked as X refers to the wild-type hemoglobin
Himanshi
August 24, 2025X refer wild type hb
Anisha Beniwal
August 24, 2025Option a is correct as wild type dna is either normal or carrier
Ayush Dubey
August 24, 2025Lane 3 represents an individual with sickle cell disease
Kajal
August 24, 2025Option a by explanation
Priyanka Verma
August 24, 2025the band x refers as the wild type hemoglobin
MOHIT AKHAND
August 24, 2025Done ✅
Parul Yadav
August 24, 2025Bands of normal hemoglobin in western blotting will be near the well while the bands of hemoglobin of sickle cell anemia will move faster and away from the well.
Sumit Chhimpa
August 24, 2025Option A is correct… Because
X band refers to the wild type haemoglobin & because of it’s heavy mol weight and also unbroken globin protein it move slow …
But having sickle cell disease suspect… The hg protein break into amino acids & here.. so due to the less mol weight it moves faster towards + chrarge… And it markked as Y band.
Surendra Doodi
August 24, 2025Band Marked as X refers to the wild type hemoglobin
Falguni Prakash
August 24, 2025A
Pooja Sharma
August 25, 2025OPTION A
Manisha choudhary
August 26, 2025Sickle cell ki condition m globin protein break hoti h jis s uska molecular weight km ho jata h too fast move kregi
(Rbc kaa bhi shape change ho jata h )
Isliye iska band niche aayega
Carrier Wale kaa band wild and disease k bich m aa skta h
Wild kaa band sbse uppr rahega
Lane 3 m band uppr h option m de rkha h individual Carrier jo ki wrong hoga individual nhi aayega kyoki lane 1 m bhi band uppr h uska bhi carrier yaa wild aa skta h
Lane 2 m individual disease wala nhi hoga kyoki lane 1 m bhi band niche ki or shift h option D m de rkha h lane 3 wala individual disease y ho ji nhi skta kyoki band uppr h
Isliye X wild type answer right hoga band uppr h
Neha Yadav
August 27, 2025The band marked as X- refers to the wild type Hb protein
Kanica Sunwalka
August 28, 2025in SCA hb – globin protein brk
+ mutated
protein -vely charged – move toward +ve charge
disease / carrier – size kam
Shriyanshi verma
August 30, 2025The band marked as X refers to the wild-type hemoglobin so band uper show
Alec
August 30, 2025The band marked as X refers to the wild type haemoglobin.
Pratibha Jain
August 30, 2025The band marked as X- refers to the wild type Hb protein
Anisha jakhar
August 31, 2025Option A is correct
Kajal
August 31, 2025Band which is X marked refer to wild type Hb.
Shobha Kanwar
August 31, 2025Option A is correct
X represent wild- type hemoglobin or may be carrier both
Deepika sheoran
November 15, 2025Option A is correct