Tag: CSIR NET Life Science Previous Year Questions on METHODS IN BIOLOGY

45. Point group symmetry operations such as inversion and mirror plane are not applicable to protein crystals. This is because (A) Protein molecules assemble in highly ordered fashion. (B) Protein […]

44. Protein conformational dynamics CANNOT be determined by which one of the following techniques? (A) NMR spectroscopy (B) Differential scanning calorimetry (C) Mass spectroscopy (D) Fluorescence microscopy Protein Conformational Dynamics: […]

43. From the following statements, A. Surface plasmon resonance can be used to determine binding constants only in the range of 10²–10³ M. B. De novo sequencing is not possible […]

42. Shown below are the CD spectra of a protein recorded under two different conditions. From the options given below, select the one that is the best interpretation of the […]

41. Protein ‘A’ was subjected to different experiments: (i) SDS-PAGE with/without β-mercaptoethanol (β-ME) (ii) Fluorescence (iii) Far-UV CD (iv) Near-UV CD spectra at pH 7.0 (black) and 3.0 (red) The […]

40. A circular dichroism spectrum in the far-UV region informs on the kind and content of secondary structures in a protein. Near-UV and tryptophan emission spectra inform on the tertiary […]

39. A researcher is investigating structural changes in a protein by following tryptophan fluorescence and circular dichroism. Fluorescence and CD spectra of the pure protein were obtained in the absence […]

38. A 100 residue long protein has a single chromophoric residue (tyrosine). The UV absorption of this protein and a homologous protein (also with a single tyrosine residue) was monitored […]

37. Cytochrome-c has only one tryptophan residue (W) which is buried. The protein in cacodylate buffer (pH 6.0) is excited at 280 nm, and its emission spectrum measured in the […]

36. The mechanism of oxygen transport by hemocyanin (containing Cu) is described by: Cu⁺ + Cu⁺ + O₂ ⇌ Cu²⁺–O₂²⁻–Cu²⁺ Which one of the following techniques can be used to […]