Tag: CSIR NET Life Science Previous Year Questions on METHODS IN BIOLOGY

18. The movement of proteins within the nucleus can be determined by: A. Electron microscopy B. Fluorescence recovery after photobleaching C. Fluorescent in situ hybridization D. Confocal light microscopy Fluorescence […]

17. You wish to localize a given gene product at subcellular levels following immunofluorescence staining. Routine microscopy could not resolve whether the gene product is localized inside the nucleus or […]

16. The movement of a single cell was required to be continually monitored during development. This cell was marked with a reporter gene. To visualize this movement one would use: […]

15. Bindin is a protein that is expressed in the tip of the sperm head (acrosome) and is important for sperm–egg interaction. Its distribution pattern changes during sperm maturation. Which […]

14. Which one among the following microscopic techniques is used to see clear resolution for the topology and distribution of transmembrane proteins of a cell membrane? A. Freeze-fracture electron microscopy […]

13. What is considered a weakness of scanning tunneling microscopy (STM)? A. The inability to move and arrange atoms to create a design B. The requirement for a conducting surface […]

12. Fluorescence microscopy is based on the ability of certain molecules to: A. Absorb light of a constant wavelength B. Absorb light of many different wavelengths C. Absorb light at […]

11. Which of the following is false about freeze-fracture technique? A. It involves physical breaking of frozen biological samples B. Fixation in glutaraldehyde C. Involves vacuum sublimation of ice D. […]

10. The kind of microscope in which image is formed by passing an electron beam through a specimen and focusing the scattered electrons is (A) Transmission electron microscope (B) Scanning […]

9. An image is formed from diffraction pattern by the process of (A) Image transformation (B) Data transformation (C) Fourier transformation A. Image transformation: Incorrect. Generic term; doesn’t specify mathematical […]