Southern Blotting-Western blotting-Northern Blotting-DNA fingerprinting
Arrange the following in correct order
1. Southern Blotting — A) RNA-DNA hybrid
2. Western blotting — B) DNA-DNA hybrid
3. Northern Blotting — C) Southern blotting
4. DNA fingerprinting — D) Antigen antibody reaction
Answer:
The correct order for the matching of blotting techniques and their corresponding molecular interactions is:
- Southern Blotting — B) DNA-DNA hybrid
- Western Blotting — D) Antigen-antibody reaction
- Northern Blotting — A) RNA-DNA hybrid
- DNA Fingerprinting — C) Southern blotting
Explanation:
Blotting techniques are essential tools in molecular biology for identifying and analyzing specific nucleic acids and proteins within a complex mixture. These techniques rely on the principle of hybridization, where a specific probe binds to a complementary target sequence or protein. Let’s explore each technique in detail:
1. Southern Blotting — DNA-DNA Hybrid
Southern blotting is used to detect specific DNA sequences in a sample. Developed by Edwin Southern in 1975, this technique involves:
- Digesting the DNA with restriction enzymes
- Separating the fragments using gel electrophoresis
- Transferring the separated DNA fragments onto a membrane (often with aminobenzyloxymethyl filter paper)
- Hybridizing with a labeled DNA probe that binds to the complementary sequence
The result is a DNA-DNA hybrid, which can be visualized using autoradiography or chemiluminescence.
2. Western Blotting — Antigen-Antibody Reaction
Western blotting is used to detect specific proteins in a sample. This technique involves:
- Separating proteins using SDS-PAGE (sodium dodecyl sulfate–polyacrylamide gel electrophoresis)
- Transferring proteins onto a nitrocellulose or PVDF membrane
- Blocking the membrane to prevent non-specific binding
- Probing with a primary antibody specific to the target protein, followed by a secondary antibody conjugated to an enzyme or fluorescent marker
The antigen-antibody interaction produces a signal that is detected through chemiluminescence or fluorescence.
3. Northern Blotting — RNA-DNA Hybrid
Northern blotting is used to detect and measure the expression of specific RNA molecules in a sample. Developed as an adaptation of Southern blotting, the process includes:
- Separating RNA molecules using gel electrophoresis
- Transferring the RNA onto a membrane using aminobenzyloxymethyl filter paper
- Hybridizing with a labeled DNA or RNA probe complementary to the target RNA sequence
The resulting RNA-DNA hybrid is visualized through autoradiography or fluorescence imaging.
4. DNA Fingerprinting — Southern Blotting Technique
DNA fingerprinting is a technique used to identify individuals based on variations in their DNA sequences. It is based on the Southern blotting technique and involves:
- Extracting DNA from a sample
- Cutting the DNA with restriction enzymes
- Separating the fragments using gel electrophoresis
- Transferring onto a membrane and hybridizing with a labeled probe specific to repetitive sequences (e.g., VNTRs – variable number tandem repeats)
The resulting pattern, or “fingerprint,” is unique to an individual and can be used for forensic analysis, paternity testing, and genetic research.
✅ Correct Order:
- Southern Blotting – DNA-DNA Hybrid
- Western Blotting – Antigen-Antibody Reaction
- Northern Blotting – RNA-DNA Hybrid
- DNA Fingerprinting – Southern Blotting
78 Comments
Arushi
April 9, 2025👍✔️
Khushi Vaishnav
August 19, 2025SB – DNA-DNA hybrid
WB- Antigen-antibody
NB – RNA-DNA hybrid
DNA Fingerprinting -Southern blotting
Mahima Sharma
August 24, 2025Southern – dna dna hybrid
Western -antibody ‘antigen
Nouthern- rna-dna hybrid
Dna fingerprint -southern blotting
Neelam Sharma
August 19, 2025Southern blotting is DNA DNA hybrid
Northern blotting is RNA DNA hybrid
Western blotting is antigen -antibody rxn
DNA fingerprinting based upon Southern blotting
Divya rani
August 20, 2025B is the right answer
Southern blotting. ….dna dna hybrid and dna finger printing application
Northern blotting. …..rna dna hybrid
Western blotting……antigen antibody interaction.
Aakansha Sharma
August 20, 2025Option B
Santosh Saini
August 20, 2025SB= DNA DNA hybrid (b) WB= antigen antibody reaction (d) NB= RNA DNA hybrid (a) DNA finger printing= SB (c), so option B is correct
Dipti Sharma
August 20, 2025Southern Blotting – DNA-DNA Hybrid
Western Blotting – Antigen-Antibody Reaction
Northern Blotting – RNA-DNA Hybrid
DNA Fingerprinting – Southern Blotting
Diksha Chhipa
August 20, 2025B is correct
Nilofar Khan
August 20, 2025Southern blotting =dna dna hybrid
Western blotting =antigen -antibody reaction
Nouthern blotting =rna-dna hybrid
Dna fingerprint=southern blotting
Asha Gurzzar
August 20, 2025B is the right answer because Western blotting is for antibody antigen, southern for dna, northern for rna,dna fingerprint is in southern blotting
Anurag Giri
August 20, 2025Option b
Southern Blotting – DNA-DNA Hybrid
Western Blotting – Antigen-Antibody Reaction
Northern Blotting – RNA-DNA Hybrid
DNA Fingerprinting – Southern Blotting
anjani sharma
August 20, 2025Answer b matches correct answers
Priya dhakad
August 20, 2025Answer b is correct
Southern blotting – DNA fingerprinting, DNA- DNA hybrid
Northern blotting -RNA -DNA hybrid
Western blotting -Ag-Ab rxn
Dharmpal Swami
August 20, 2025Option B write
Karishma
August 20, 2025southern blotting-> DNA-DNA HYBRID
western blotting-> Antigen-Antibody reaction
northern blotting-> rna-dna hybrid
dna fingerprinting-> southern blotting
Mansukh Kapoor
August 20, 2025The correct answer is option 2nd
Kavita choudhary
August 20, 2025Answer b is correct
Khushi Singh
August 20, 2025b is correct
HIMANI FAUJDAR
August 20, 2025Ans southern blotting – DNA – DNA hybrid
Northern blotting – RNA -DNA hybrid
Western blotting – Antigen Antibody interaction
DNA fingerprinting – southern blotting
Priya khandal
August 20, 20252 is right
Priti khandal
August 20, 2025Correct answer is b
Komal Pareek
August 20, 2025B is correct
Deepika Sheoran
August 21, 2025Option b
Southern blotting – DNA DNA hybrid
Western blotting – Antigen Antibody Reaction
Northern blotting -RNA DNA hybrid
DNA Fingerprinting -Southern blotting.
Meenakshi Choudhary
August 21, 2025Option b is correct
Southern Blotting – DNA-DNA Hybrid
Western Blotting – Antigen-Antibody Reaction
Northern Blotting – RNA-DNA Hybrid
DNA Fingerprinting – Southern Blotting
Mohd juber Ali
August 21, 2025Option b is right
Payal Gaur
August 21, 2025Option b correct
Southern blotting- DNA-DNA hybrid
Northern blotting- DNA-RNA hybrid
Western blotting- antigen antibody reaction
Meera Gurjar
August 21, 2025Ans B is right
Sakshi yadav
August 21, 2025Southern Blotting — DNA-DNA hybrid
Western Blotting — Antigen-antibody reaction
Northern Blotting — RNA-DNA hybrid
DNA Fingerprinting — Southern blotting
Ankita Pareek
August 21, 2025SB- dna DNA hybrid
NB- rna dna hybrid
WB- antigen antibody reaction
Dna fingerprinting – southern blotting
Bharti Yadav
August 21, 2025Southern Blotting — DNA-DNA hybrid
Western Blotting — Antigen-antibody reaction
Northern Blotting — RNA-DNA hybrid
DNA Fingerprinting — Southern blotting
Sonam Saini
August 21, 2025Right b
Bhawna Choudhary
August 21, 2025The correct answer is B
Aafreen Khan
August 22, 2025B is correct answer
1-Southern Blotting – DNA-DNA Hybrid
2-Western Blotting – Antigen-Antibody Reaction
3-Northern Blotting – RNA-DNA Hybrid
4-DNA Fingerprinting – Southern Blotting
shruti sharma
August 22, 2025option B
Aman Choudhary
August 22, 2025Southern Blotting — DNA-DNA hybrid
Western Blotting — Antigen-antibody reaction
Northern Blotting — RNA-DNA hybrid
DNA Fingerprinting — Southern blotting
Simran Saini
August 22, 2025Southern blotting – DNA-DNA hybrid.
Western blotting – Antigen-Antibody reaction.
Northern blotting – RNA-DNA hybrid.
DNA fingerprinting – southern blotting.
Kirti agarwal
August 22, 2025Opt. B
Avni
August 22, 2025correct Answer is option (b)
Sakshi
August 22, 2025B is correct option
Southern blotting – Dna-Dna hybrid
Northern blotting -Rna -Dna hybrid
Western blotting – Antigen -Antibody binding
Dna fingerprinting is application of southern blotting
Mitali saini
August 23, 2025The correct order for the matching of blotting techniques and their corresponding molecular interactions is:
Southern Blotting — B) DNA-DNA hybrid
Western Blotting — D) Antigen-antibody reaction
Northern Blotting — A) RNA-DNA hybrid
DNA Fingerprinting — C) Southern blotting
Soniya Shekhawat
August 23, 2025Correct option is B
Priyanka Choudhary
August 23, 2025Right answer is option b,
Southern Blotting – DNA-DNA Hybrid
Western Blotting – Antigen-Antibody Reaction
Northern Blotting – RNA-DNA Hybrid
DNA Fingerprinting – Southern Blotting
Niti Tanwar
August 23, 2025Right answer is b
Southern blotting -dna-dna hybrid
Western blotting – antigen antibody reaction
Northern blotting -rna – dna hybrid
Dna finger print – southern blotting
Varsha Tatla
August 23, 2025Dna finger printing -southern blotting
Northern blotting -dna rna blotting
Southern blotting -dna dna blotting
Western blotting -antibody antigen binding
Akshita Jangid
August 23, 2025option B is correct
Kajal
August 23, 2025Option b is correctly explain the following question .
Southern blotting for DNA -DNA hybrid.
Western blotting used for antigen antibody interaction.
Northern blotting for rna -dna hybrid.
Dna finger printing uses southern blotting.
Sakshi Kanwar
August 23, 2025Southern Blotting – DNA DNA hybrid
Western Blotting – Antigen-antibody reaction
Northern Blotting – RNA DNA hybrid
DNA Fingerprinting – Southern blotting
Option B
Divya Mandhania
August 23, 2025Option b
Yogita
August 24, 2025Southern blotting -dna-dna hybrid,
Western blotting -antigen -antibody reaction
Northern blotting -RNA-Dna hybrid
DNA fingerprinting – southern blotting
Shobha Sharma
August 24, 2025S.b – DNA – DNA hybrid
W.b – antigen antibody reaction
N.b – RNA -DNA
DNA fingerprinting – s.b
Arushi Saini
August 24, 2025Southern :- DNA – DNA
Northern :- DNA – RNA
Western :- antigen – antibody
Dna finger printing :- southern blotting
Sneha Kumawat
August 24, 2025surthern Bloating-DNA DNA hybrid
Western bloating-antigen antibody reaction
Northern blotting – rna DNA hybrid
DNA fingerprinting-surthern blotting
Alec
August 24, 2025option b is the correct ans.
AKANKSHA RAJPUT
August 24, 2025Southern Blotting — B. DNA-DNA hybrid
Western Blotting — D. Antigen-antibody reaction
Northern Blotting — A. RNA-DNA hybrid
DNA Fingerprinting — C. Southern blotting
Shivani
August 24, 2025Southern Blotting – DNA-DNA Hybrid
Western Blotting – Antigen-Antibody Reaction
Northern Blotting – RNA-DNA Hybrid
DNA Fingerprinting – Southern Blotting
Himanshi
August 24, 2025B
Anisha Beniwal
August 24, 2025Option B
Neeraj Sharma
August 24, 2025Option B is correct
Devika
August 24, 2025Option B
Pooja Sharma
August 24, 2025OPTION B
Sonam Saini
August 24, 2025Option B is right
Falguni Prakash
August 24, 2025B
Swati Choudhary
August 24, 2025Southern Blotting – DNA-DNA Hybrid
Western Blotting – Antigen-Antibody Reaction
Northern Blotting – RNA-DNA Hybrid
DNA Fingerprinting – Southern Blotting
Ajay Sharma
August 25, 2025b is correct
Kashish yadav
August 25, 2025Ok sir
Kajal
August 25, 2025Southern blotting – dna : dna hybrid
Western blotting – antigen – antibody
Northern blotting – rna :dna hybrid
Dna fingerprint – southern blotting
Bhavana kankhedia
August 26, 2025Southern Blotting – DNA-DNA Hybrid
Western Blotting – Antigen-Antibody Reaction
Northern Blotting – RNA-DNA Hybrid
DNA Fingerprinting – Southern Blotting
Anju
August 26, 2025Ans:b
Muskan Yadav
August 26, 2025Option b is correct Southern Blotting – DNA-DNA hybrid
Western Blotting – Antigen-antibody reaction
Northern Blotting – RNA-DNA hybrid
DNA Fingerprinting- Southern blotting
Kanica Sunwalka
August 28, 2025option b is correct
Komal Sharma
August 28, 2025Option 2 is correct
Shriyanshi verma
August 30, 2025SB – DNA-DNA hybrid
WB- Antigen-antibody
NB – RNA-DNA hybrid
DNA Fingerprinting -Southern blotting
Pratibha Jain
August 30, 2025Correct answer is option (b)
Southern Blotting – DNA-DNA hybrid
Western Blotting – Antigen-antibody reaction
Northern Blotting – RNA-DNA hybrid
DNA Fingerprinting- Southern blotting
Anjali
August 31, 2025Southern Blotting – DNA-DNA hybrid
Western Blotting – Antigen-antibody reaction
Northern Blotting – RNA-DNA hybrid
DNA Fingerprinting- Southern blotting
Reply
Anisha jakhar
August 31, 2025Southern blotting- DNA- DNA hybrid
Western blotting- antigen- antibody reaction
Northern blotting- RNA- DNA hybrid
DNA fingerprinting- Southern blotting
Shobha Kanwar
August 31, 2025Answer b is correct
Southern blotting – DNA fingerprinting, DNA- DNA hybrid
Northern blotting -RNA -DNA hybrid
Western blotting -Ag-Ab reaction
Divyanshi Vaishnav
September 1, 2025Southern blotting : dna dna hybid
Northern blotting : antigen antibody
Western blotting : rna dna hybrid
Dna fingerprinting : southern blotting