Aminobenzyloxymethyl Filter Paper in Northern Blotting

Aminobenzyloxymethyl filter paper is commonly used for transfer in

A. Western blotting
B. Southern blotting
C. Northern blotting
D. Dot blotting

Answer:
Aminobenzyloxymethyl (ABM) filter paper is commonly used for transfer in Northern blotting.

Explanation:

Northern blotting is a molecular biology technique used to detect specific RNA sequences within a sample. Aminobenzyloxymethyl (ABM) filter paper is often used during the transfer step in Northern blotting because of its high binding affinity for RNA molecules. The ABM modification enhances the paper’s capacity to immobilize RNA fragments effectively, ensuring efficient hybridization with labeled RNA probes.

During Northern blotting, RNA is separated using gel electrophoresis and then transferred onto a membrane with the help of ABM filter paper. This paper helps maintain the integrity and positioning of the RNA fragments, allowing for accurate and clear detection of target RNA sequences during hybridization.

✅ Correct Answer: C. Northern blotting