38. A conjugation experiment is carried out between F+ his+ leu+ thr+ pro+ bacteria and F– his– leu– thr– pro– bacteria for a period of 25 minutes. At this time […]
Using interrupted mating, four Hfr strains were analysed for the sequence in which they transmitted a number of different genes to a F- strain. Each Hfr strain was found to […]
In a conjugation experiment between bacterial Hfr strain ‘X’ and F-cell, lac gene enters the recipient in 4 minutes, but the F-cells remain auxotrophic for Leu, Trp, Ura, Glu,Phe and […]
33. A cross is mode between an Hfr strain that is met+ thi+ pur+ and an F– that is met– thi– pur–. The recombinants are selected on medium that contains […]
32. A cross was made between Hfr met+ arg+ leu+ strs X F– met– arg– leu– strr in which leu+ exconjugants are selected. If the linear organization of the genes […]
31. Two Hfr strains, Hfr-1 (arg+leu+ gal+strS) and Hfr-2 (arg+ his+ gal+ pur+ strs) were mated with a F– strain (arg–leu– gal– his–pur–strs). The results of the interrupted mating experiment […]
30. Four different Hfr strains of E. coli were mated with F– recipients, and the time of entry of various donor markers were found to be as below: Hfr 1: […]
Interrupted mating experiments were performed using three different Hfr strains (1-3). The three strains have different combinations of selectable markers. The time of entry for markers for each strain is […]