Q.62 An enzyme is immobilized on the surface of a non-porous spherical particle of 2 mm diameter. The immobilized enzyme is suspended in a solution having bulk substrate concentration of […]
Q.61 A protein is to be purified using ion-exchange column chromatography. The relationship between HETP (Height Equivalent to Theoretical Plate) and the linear liquid velocity of mobile phase is given […]
Q.60 An enzyme converts substrate A to product B. At a given liquid feed stream of flow rate 25 L·min−1 and feed substrate concentration of 2 mol·L−1, the volume of […]
Q.59 A bioreactor is scaled up based on equal impeller tip speed. Consider the following parameters for small and large bioreactors: Parameters Small Bioreactor Large Bioreactor Impeller speed N1 N2 […]
Q.58 Decimal reduction time of bacterial spores is 23 min at 121 °C and the death kinetics follow first order. One liter of medium containing 105 spores per mL was sterilized […]
Q.57 Saccharomyces cerevisiae is cultured in a chemostat (continuous fermentation) at a dilution rate of 0.5 h−1. The feed substrate concentration is 10 g·L−1. The biomass concentration in the chemostat […]
Q.56 The empirical formula for biomass of an unknown organism is CH1.8O0.5N0.2. To grow this organism, ethanol (C2H5OH) and ammonia are used as carbon and nitrogen sources, respectively. Assume no […]