Which of the following statements about SDS PAGE is correct ? a) Wanted proteins can be tested for their biological activity after separation by SDS PAGE (b) Proteins are solubilised […]
Tag: SDS-PAGE
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CSIR NET Previous Year Questions Gel Electrophoresis
SDS-PAGE -Sodium Dodecyl Sulfate – Polyacrylamide Gel Electrophoresis
In SDS PAGE the protein sample is first (a) Treated with reducing agent and then with anionic detergent followed by fractionation by electrophoresis (b) Fractionated by electrophoresis then treated with […]
CSIR NET Previous Year Questions Gel Electrophoresis
Proteins A and B having a molecular weight of 16500 and 35400 move 1.3 cm and 4.6 cm
Proteins A and B having a molecular weight of 16500 and 35400 move 1.3 cm and 4.6 cm, respectively, when electrophoresed through a gel. What is the molecular weight of […]
CSIR NET Previous Year Questions Gel Electrophoresis
The relative electrophoretic mobilities of a 30-kd protein and a 92-kd protein used as standards on an SDS
The relative electrophoretic mobilities of a 30-kd protein and a 92-kd protein used as standards on an SDS polyacrylamide gel are 0.80 and 0.41, respectively. What is the apparent mass […]
CSIR NET Previous Year Questions Gel Electrophoresis
SDS Used in the Electrophoresis of Proteins
Why is SDS used in the electrophoresis of proteins? a. SDS coats the protein with a negative charge so that the sample can run through the gel. b. SDS is […]
