In SDS PAGE the protein sample is first
(a) Treated with reducing agent and then with anionic detergent followed by fractionation by electrophoresis
(b) Fractionated by electrophoresis then treated with an oxidizing agent followed by anionic detergent
(c) Treated with a oxidizing agent and then with anionic detergent followed by fractionation by electrophoresis
(d) None of the above
✅ Correct answer: (A) Treated with reducing agent and then with anionic detergent followed by fractionation by electrophoresis
Explanation:
In SDS-PAGE (Sodium Dodecyl Sulfate – Polyacrylamide Gel Electrophoresis), the protein sample undergoes the following steps before electrophoresis:
-
Treatment with a reducing agent (e.g., β-mercaptoethanol or dithiothreitol, DTT)
- This breaks disulfide bonds in proteins, ensuring that subunits separate completely.
-
Treatment with an anionic detergent (SDS – Sodium Dodecyl Sulfate)
- SDS denatures proteins and coats them with a uniform negative charge, so their migration in the gel depends only on size (molecular weight) and not on charge.
-
Electrophoresis in polyacrylamide gel
- The negatively charged proteins migrate towards the positive electrode, with smaller proteins moving faster through the gel matrix.
Why Not the Other Options?
-
(B) Fractionated by electrophoresis then treated with an oxidizing agent followed by anionic detergent ❌
- Electrophoresis is done after sample preparation, not before.
- Oxidizing agents are not used in SDS-PAGE; reducing agents are used to break disulfide bonds.
-
(C) Treated with an oxidizing agent and then with anionic detergent followed by fractionation by electrophoresis ❌
- Oxidizing agents do not break disulfide bonds; instead, reducing agents (β-mercaptoethanol, DTT) are required.
-
(D) None of the above ❌
- Option (A) is correct, so this is incorrect.
Final Answer:
✅ (A) Treated with reducing agent and then with anionic detergent followed by fractionation by electrophoresis
82 Comments
Parul
February 25, 2025Done sir. Thankyou for making things easy to understand.
Mahima Sharma
August 24, 2025First give Temp. And sds gives neg charge to protein and then gel
Nisha
February 25, 2025✅ done
Mahima Sharma
August 16, 2025Anser is a
Alina mansuri
February 25, 2025Didn’t get it
Ujjwal
February 25, 2025Nice sir
Shreeji Charan
February 26, 2025Was correct
Lavanya Sharma
February 26, 2025👍🏻
Suman bhakar
February 26, 2025Was correct
Ashwini Shekhawat
February 26, 2025Done
Lokesh kumawat
February 26, 2025Done
Abhilasha
February 26, 2025Done sir ✅
Arushi
February 26, 2025☑️👍
Vikram
February 26, 2025Ok sir 👍
Pooja jat
February 27, 2025done.
Kabeer Narwal
February 27, 2025👍
Prami Masih
February 27, 2025Done sir
yogesh sharma
February 27, 2025Great explanation sir 🙏
Pallavi
February 27, 2025Done sir
yogesh sharma
February 27, 2025Easily explained
Thank you sir for making efforts for us.
Priti khandal
March 2, 2025Done sir
Jyoti Meena
March 24, 2025Done
Mohd juber Ali
August 14, 2025Option (A)
Firstly treated with reducing agent BME( di sulphide bond break)
Then SDS detergent (anionic), denature protein
Khushi Agarwal
August 14, 2025Option a is correct
Treated with reducing agent and then with anionic detergent followed by fractionation by electrophoresis
First denatured – anionic detergent – electrophoresis
Aman Choudhary
August 14, 2025option A is correct
Firstly treated with reducing agent by which protein denature then SDS give negative charge and then electrophoresis
Dharmpal Swami
August 14, 2025A is correct
Anjani sharma
August 14, 2025Answer A is correct, treated with reducing agent and then with anionic detergent then electrophoresis
Neelam Sharma
August 14, 2025Treated by reducing agent di sulphide bond break and anionic detergent followed by fractionation by electrophoresis
Sneha Kumawat
August 14, 2025Answer a is correct
Akshita Saini
August 14, 2025Correct answer A
Treated with reducing agent and then with anionic detergent followed by fractionation by electro.
Soniya Shekhawat
August 14, 2025A is correct option
Mansukh Kapoor
August 14, 2025The correct answer is option 1st
In SDS Page the sample first treated with reducing agent to break down of di sulphide bonds and then with anionic detergent (SDS) to provide uniform negative charge and than fractionation by electrophoresis
Dipti Sharma
August 14, 2025Treated with reducing agent and then with anionic detergent then electrophoresis
Karishma Don
August 14, 2025protein is reduced by a reducing agent which is beta-mercaptoethanol and then SDS denatures and give -ve charge to the protein.
Deepika Sheoran
August 14, 2025Option A is correct answer
Treated with reducing agent & then with anionic detergent followed by fractionation by electrophoresis.
Priti khandal
August 14, 2025the correct answer is a because sds page treated with reducing agent and then with ainionic detergent followed by fractionaction by electrophoresis
Priya khandal
August 14, 2025Treted with reducing agent and then w8th anionic detergent forwed by fractiontion by elec
Divya rani
August 16, 2025A is correct because anionic detergent is SDS and reducing agent is firstly used to treat the protein
Aafreen
August 16, 2025Treated with reducing agent and then with anionic detergent followed by fractionation by electrophoresis
shruti sharma
August 16, 2025ans a
Kavita choudhary
August 17, 2025Answer a is right treated with redusing agent and them anionic detergent followed by fractionation by electrophoresis
Parul Yadav
August 17, 2025Protein sample is first treated with reducing agent and than with anionic detergent to carry out electrophoresis in polyacrylamide gel.
Kajal
August 17, 2025Option – A
Treated with reducing agent then with anionic detergent then electrophoresis
Khushi Vaishnav
August 17, 2025Treated with reducing agent and then with anionic detergent followed by fractionation by electrophoresis
Avni
August 17, 2025Answer is option (A) Treated with reducing agent and then with anionic detergent followed by electrophoresis
Meenakshi Choudhary
August 18, 2025Treated with reducing agent and then with anionic detergent followed by fractionation by electrophoresi
Simran Saini
August 18, 2025Option A.
Treated with reducing agent and then with anionic detergent followed by fractionation by electrophoresis.
Varsha tatla
August 19, 2025Got it
Manisha choudhary
August 20, 2025Sds page k liye sbse phle reducing agent s treat krte h jaise beta mercaptoethanol ,dithiothritol DTT jo protein m disulphide bond break kr deta h jis s protein ki subunit alag alag ho jati h
Then sds detergent s treat Kiya jata h jo protein ko denature kr uniformly negative charge provide krta h jo protein ko mass k base p sepration ko allow krta h
Negatively charged protein positive electrode ki or move krti km mass wali protein fast move kregi and jyada maas waali protein slow move kregi
Komal Sharma
August 21, 2025Treated with reducing agent and then with anionic detergent followed by fractionation by electrophoresis.
Bhavana kankhedia
August 24, 2025Treated with reducing agent and then with anionic detergent followed by fractionation by electrophoresis.
Akshita Jangid
August 24, 2025Option A
Treated with reducing agent and then with anionic detergent followed by fractionation by electrophoresis
Bharti Yadav
August 24, 2025Treated with reducing agent and then with anionic detergent followed by fractionation by electrophoresis
Priyanka Choudhary
August 24, 2025Option A is correct answer, because of this
Treated with reducing agent and then with anionic detergent followed by fractionation by electrophoresis
Priyanka Choudhary
August 24, 2025Correct answer is a,
Treated with reducing agent and then with anionic detergent followed by fractionation by electrophoresis
Urmi sisodiya
August 24, 2025Answer a is correct
Treatment with reducing agent such as betamercapethanol thena anionic detergent used
Ankita Pareek
August 24, 2025In sds the protein is first treated with reducing agent so that disulfide bonds break then treat with sds or anionic detergent it denatures the protein and gives it uniform negative charge so that in gel proteins move according to their molecular weight no charge
Sonam Saini
August 24, 2025Option A is right
Alec
August 24, 2025correct ans is (a)
Priya dhakad
August 24, 2025Treated with reducing agent and then with anionic detergent followed by fractionation by electrophoresis
Devika
August 25, 2025Option A .
TREATED WITH Reducing agen then with anionic detergent followed by fractionation by electrophoresis
Falguni Prakash
August 24, 2025A
Sakshi
August 24, 2025In SDSs page the protein sample is first treated with reducing agent and then with an ionic detergents followed by fractionalization of electrophoresis
Kashish yadav
August 25, 2025Option a
sakshi khatri
August 25, 2025a is correct
Vijay kumar Meena
August 25, 2025A done ✔️ 🙌
Ayush Dubey
August 25, 2025Treated with reducing agent and then with anionic detergent followed by fractionation by electrophoresis
Anju
August 25, 2025Ans:A
shruti sharma
August 25, 2025Option – A
Treated with reducing agent then with anionic detergent then electrophoresis
Savita Garwa
August 29, 2025option A is correct
Firstly treated with reducing agent by which protein denature then SDS give negative charge and then electrophoresis
Pratibha Jain
August 30, 2025Correct answer is option (A)
Treated with reducing agent and then with anionic detergent followed by fractionation by electrophoresis
Sheikh Aleen
August 30, 2025Treated with reducing agent and then with anionic detergent followed by fractionation by electrophoresis
Anisha Beniwal
August 30, 2025Treated with reducing agent and then with anionic detergent followed by fractionation by electrophoresis
Neeraj Sharma
August 30, 2025In SDS PAGE the sample will first treated with reducing agent then with Anionic detergent and then electrophoresis is done
Vanshika Sharma
August 31, 2025In sds the sample is first treated with reducing agent then with anionic detergent and then electrophoresis is done
Shobha Kanwar
August 31, 2025Answer A is correct, treated with reducing agent and then with anionic detergent then electrophoresis
Asha Gurzzar
September 1, 2025A is correct, treatment with reducing agent and then with anionic detergent followed by fractionation by electrophoresis
Ajay Sharma
September 2, 2025Option 1 is correct
Anisha jakhar
September 3, 2025(A) Treated with reducing agent and then with anionic detergent followed by fractionation by electrophoresis
Muskan Yadav
September 3, 2025Treated with reducing agent and then with anionic detergent followed by fractionation by electrophoresis
Somya Yadav
September 5, 2025Treated with reducing agent followed by anionic detergent followed by electrophoresis
Aakansha sharma Sharma
October 1, 2025Treated with reducing agent followed by anionic detergent followed by electrophoresis