Which of the following statements about SDS PAGE is correct ?
a) Wanted proteins can be tested for their biological activity after separation by SDS PAGE
(b) Proteins are solubilised but not denatured when separated by SDS PAGE
(c) It separates protein on the basis of charge
(d) It separates protein on the basis of size
✅ Correct answer: (D) It separates protein on the basis of size
Explanation:
SDS-PAGE (Sodium Dodecyl Sulfate – Polyacrylamide Gel Electrophoresis) is a widely used technique to separate proteins based on their molecular weight (size).
- SDS (Sodium Dodecyl Sulfate) is an anionic detergent that denatures proteins and coats them with a uniform negative charge.
- This removes charge-based differences, ensuring that proteins migrate through the gel only based on their size (molecular weight).
- Smaller proteins move faster and travel farther in the gel, while larger proteins move slower.
Why Not the Other Options?
-
(A) Wanted proteins can be tested for their biological activity after separation by SDS-PAGE ❌
- Incorrect because SDS denatures proteins, meaning they lose their biological activity.
- For functional activity, native PAGE is used instead of SDS-PAGE.
-
(B) Proteins are solubilized but not denatured when separated by SDS-PAGE ❌
- Incorrect because SDS completely denatures proteins by unfolding their structure.
-
(C) It separates protein on the basis of charge ❌
- Incorrect because SDS gives all proteins a uniform negative charge, eliminating charge-based separation.
- IEF (Isoelectric Focusing) separates proteins based on charge, not SDS-PAGE.
Final Answer:
✅ (D) It separates protein on the basis of size
72 Comments
Akshay mahawar
February 25, 2025Done👍
Mahima Sharma
August 24, 2025It seprate protein on the basis of size
Nisha
February 25, 2025Done
Suman bhakar
February 26, 2025Done
Arushi
February 26, 2025☑️
Parul
February 27, 2025Question is understood. Done
Pooja jat
February 27, 2025Done .
Pooja jat
February 27, 2025Done.
Lavanya Sharma
February 27, 2025✅
Prami Masih
February 27, 2025Right sir
yogesh sharma
February 28, 2025Dine sir
Lokesh kumawat
March 1, 2025Done
Abhilasha
March 1, 2025Correct ✅
VIKRAM GAHLOT
March 7, 2025👍
Aman Choudhary
August 13, 2025option d is correct it separate the protein on the basis of size
small protein move faster n gel bcz of low mw and large protein move slower
Mohd juber Ali
August 14, 2025SDS PAGE separate protein on the basis of mass
Anurag Giri
August 14, 2025Ans d sds separate protein on the basis of size (mw)
Khushi Agarwal
August 14, 2025Option d is correct
Seperate by size
Dharmpal Swami
August 14, 2025SDS page separate protein based on their size
Priya dhakad
August 14, 2025Its seprate based on their size
Anjani sharma
August 14, 2025Answer d sds separate on the basis of size
Sneha Kumawat
August 14, 2025It seperate protein basis of size
Soniya Shekhawat
August 14, 2025Done sir✅
Mansukh Kapoor
August 14, 2025The correct answer is option 4th because SDS Page helps in to separation on the basis of the molecular weight
shruti sharma
August 14, 2025ans d
Karishma Don
August 14, 2025SDS-PAGE separates protein on the basis of size
Dipti Sharma
August 14, 2025(D) It separates protein on the basis of size
Neelam Sharma
August 14, 2025separates protein on the basis of size
Deepika Sheoran
August 14, 2025Option d is correct answer
SDS saprate on the basis of size.
Priti khandal
August 14, 2025It separate the protein on the basic of size
Priya khandal
August 14, 2025It’s separate protein on basing on size
Devika
August 15, 2025Done
Shivani
August 15, 2025Sds page separates protein on the basis of their size/ mass so option d is correct
Divya rani
August 16, 2025And is SDS page is only depend on size or mass (molecular weight) of the molecule and IEF is only based on charge
Aafreen
August 17, 2025SDS PAGE separates on the basis of size
Payal Gaur
August 17, 2025Option D. SDS page separate on the basis of size /mass
Kavita choudhary
August 17, 2025It’s separate protein on the basis of size
Kajal
August 17, 2025It separate protein on the basis of size
Avni
August 17, 2025It separate protein on the basis of their size
Simran Saini
August 18, 2025It separates protein on the basis of size.
Neeraj Sharma
August 18, 2025SDS PAGE separates the proteins on the basis of their size hence option D is correct
Manisha choudhary
August 20, 2025Sds page k baad protein biological activity lose kr deta h kyoki sds denaturant h protein ko complete denature kr deta h unfolded ho jaati and uniformly negative charge provide krta h isliye mass k base p separate allow krta h charge k base p nhi
Bhavana kankhedia
August 24, 2025it separate protein on the basis of size
Bharti Yadav
August 24, 2025(D) It separates protein on the basis of size
Priyanka Choudhary
August 24, 2025separates protein on the basis of size correct answer
Urmi sisodiya
August 24, 2025SDS seperate the protein on basis of their size
Sonam Saini
August 24, 2025It separates proteins based on their size
Alec
August 24, 2025It separates proteins on the basis of their size.
Niti Tanwar
August 24, 2025Option d it separate protein based on size.
Ankita Pareek
August 24, 2025It separates protein on the basis of size
Varsha Tatla
August 24, 2025SDS page seperates protein on the basis 5 size or mass not charge
Kashish yadav
August 25, 2025👍👍
sakshi khatri
August 25, 2025done
Vijay kumar Meena
August 25, 2025separates protein on the basis of size so D ✔️
Ayush Dubey
August 25, 2025It separates protein on the basis of size
Anju
August 25, 2025Ans: D
Devika
August 25, 2025It separates protein on basis of size
Sakshi
August 25, 2025It seperate protein on tujhe basis of size
Savita Garwa
August 29, 2025SDS-PAGE separates protein on the basis of size
Pratibha Jain
August 30, 2025Correct answer is option (D)
It separates protein on the basis of size
Sheikh Aleen
August 30, 2025It separates protein on the basis of size
Anisha Beniwal
August 31, 2025It separates protein on the basis of size
Vanshika Sharma
August 31, 2025It’s seperates protiens on the basis of size
Komal Sharma
August 31, 2025SDS-PAGE (Sodium Dodecyl Sulfate – Polyacrylamide Gel Electrophoresis) is a widely used technique to separate proteins based on their molecular weight (size).
Shobha Kanwar
August 31, 2025Correct answer is (D) It separates protein on the basis of size
Akshita Jangid
September 1, 2025It separate protein on the basis of size
Asha Gurzzar
September 1, 2025D will correct,sds page seperate proteins on basis of their size
Anisha jakhar
September 3, 2025(D) It separates protein on the basis of size
Muskan Yadav
September 3, 2025It separates protein on the basis of size.
Somya Yadav
September 5, 2025It separates protein on the basis of size
Ajay Sharma
September 6, 2025Sds denature the protein
So disable their biological activity
Make them negatively charged so the separation just based on size
More size less mobility
Aakansha sharma Sharma
October 1, 2025Correct answer: (D) It separates protein on the basis of size