Tag: CSIR NET Life Science Previous Year Questions on METHODS IN BIOLOGY

63. A researcher separates the tm of P40 (40 kDa) from a mixture of proteins P-60 (60 kDa) using a Sephadex G-100 column. A. P40 will run faster B. P60 […]

62. In order to separate red and white blood cells, which of the following methods can be used? A. Ion-exchange chromatography and FACS B. Hydrophobic chromatography and density gradient centrifugation […]

61. Ribonuclease A (RNase A) consists of 124 amino acid residues. RNase A is cleaved by subtilisin (a protease) in a quantitative fashion at the Ala 20 – Ser 21 […]

60. A solution containing Aspartic acid (pI = 2.98), Glycine (pI = 5.97), Threonine (pI = 6.53) and Lysine (pI = 9.74) in 50 mM citrate buffer (pH 3.0) was […]

59. In which type of chromatography are ion-pairing agents used for elution? A. Hydrophobic interaction chromatography B. Reverse phase chromatography C. Ion exchange chromatography D. Immobilized metal anion chromatography Answer: […]

58. One of these purification steps requires initial high ionic strength in the sample: A. Ion exchange chromatography B. Hydrophobic interaction chromatography C. Chromatofocusing D. Preparative chromatography Answer: (B) Hydrophobic […]

57. Carboxymethyl cellulose is: A. A cation-exchange matrix B. A gel filtration matrix C. An anion-exchange matrix D. A plant cell wall constituent Answer: (A) A cation-exchange matrix Carboxymethyl cellulose […]

56. Affinity chromatography is a method that can be used to purify cell surface receptors, while they retain their hormone binding ability. A ligand (hormone) for a receptor of interest […]

55. Two monomeric proteins migrate identically in SDS-polyacrylamide gel electrophoresis, but get separated in a gel-permeation chromatography experiment. This could be because: A. They have different isoelectric points B. One […]

54. Which would be best to separate a protein that binds strongly to its substrate? A. Gel filtration B. Affinity chromatography C. Cation exchange D. Anion exchange Correct Answer: B. […]