15. The emission maximum of tryptophan fluorescence in a protein is ~335 nm. This suggests that tryptophan (1) is in a hydrophobic environment. (2) occurs in a helical segment. (3) […]
Tag: CSIR NET Life Science Previous Year Questions on METHODS IN BIOLOGY
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CSIR NET Life Science Previous Year Questions and Solutions Spectroscopy
Why is Fluorescence Emission Shifted to a Longer Wavelength?
14. Emission maximum of a fluorophore is shifted to longer wavelength when compared to the wavelength of excitation. What is the reason? (1) Non-radiative loss of excitation energy (2) Partial […]
CSIR NET Life Science Previous Year Questions and Solutions Spectroscopy
How to Calculate Optical Density (OD)
13. A solution contains NADH and NAD+, BOTH AT 0.1 mM concentration. If NADH has a molar extinction coefficient of 6220 and that of NAD+ is negligible, the optical density […]
CSIR NET Life Science Previous Year Questions and Solutions Spectroscopy
How to Calculate Tryptophan and Tyrosine Content
12. The values of molar absorption coefficient (ε) of Trp and Tyr at 240 nm and 280 nm are the following: Wavelength (nm) εTyr (M−1 cm−1) εTrp (M−1 cm−1) 240 […]
CSIR NET Life Science Previous Year Questions and Solutions Spectroscopy
How to Calculate Protein Absorbance at 280 nm
11. A protein contains 2 Trp and 4 Tyr residues. The molecular mass of the protein is 17000 Da and that of Trp and Tyr are 204 and 180 Da, […]
CSIR NET Life Science Previous Year Questions on METHODS IN BIOLOGY – Video and Text Solutions
Scanning Probe Microscope Properties
28. Which property is measured with a scanning probe microscope? A. Magnetism B. Electric resistance C. Light absorption D. All of the above Scanning probe microscopes (SPMs) excel at nanoscale […]
CSIR NET Life Science Previous Year Questions on METHODS IN BIOLOGY – Video and Text Solutions
Microscopy Techniques Correct Statements
27. From the following statements: A. Coloured images can be obtained by transmission electron microscopy by fluorescent labelling of the specimen B. Scanning electron microscopy requires sectioning of the sample […]
CSIR NET Life Science Previous Year Questions on METHODS IN BIOLOGY – Video and Text Solutions
Fluorescence Microscope Best Resolution
26. To achieve the best resolution using a fluorescence microscope, what combination of wavelength of emitted light (λ), refractive index and the angle (2θ) by which light enters into the […]
CSIR NET Life Science Previous Year Questions on METHODS IN BIOLOGY – Video and Text Solutions
Fluorescence Microscope Live
25. Which one of the following microscopes would you use to visualize a protein fused to an appropriate reporter in a living cell? A. Fluorescence microscope B. Scanning electron microscope […]
CSIR NET Life Science Previous Year Questions on METHODS IN BIOLOGY – Video and Text Solutions
Microscope Resolution Formula Improvement
24. The most important property of any microscope is its resolution (D) and can be calculated from the formula: D = 0.61λ / (µ sin θ) where D is the minimum distance […]
