Tag: CSIR NET Life Science Previous Year Questions on METHODS IN BIOLOGY

73. You are studying the binding of proteins to the cytoplasmic face of cultured liver cells and have found a method that gives a good yield of inside-out vesicles from […]

72. Match the chromatographic technique from Group X with the appropriate elution conditions given in Group Y: Group X A. DEAE-Sephadex B. Chromatofocusing C. Phenyl Sepharose D. G-150 Sephadex Group […]

71. In which type of chromatography are ion-pairing agents used for elution? A. Hydrophobic interaction chromatography B. Reverse phase chromatography C. Ion exchange chromatography D. Immobilized metal anion chromatography Reverse […]

70. Nickel nitrilotriacetic acid columns are used in __________ chromatography. A. Ion exchange B. Affinity C. Size exclusion D. Reverse phase The correct answer is B. Affinity. Nickel nitrilotriacetic acid […]

69. Which one of the following purification steps always requires a high initial ionic strength in the sample? A. Ion exchange chromatography B. Hydrophobic interaction chromatography C. Chromatofocusing D. Gel […]

68. In a gel filtration chromatography: A. The large protein will be eluted first B. The small protein will be eluted first C. Both large and small will elute at […]

67. Which type of matrix is involved by Carboxymethyl cellulose among the following options: A. Anion-exchange matrix B. Plant cell wall constituent C. Cation-exchange matrix D. Gel filtration matrix Carboxymethyl […]

66. A mixture of proteins namely P, Q, R and S having molecular mass 50, 80, 120 and 150 kDa is applied on the Sephadex G-200 column. The order of […]

65. Separation of DNA molecules labelled with 15N and 14N can be accomplished by: A. Capillary electrophoresis B. Differential centrifugation C. Pulse field gel electrophoresis D. Density gradient ultracentrifugation The […]

64. Optical density of 1 means: A. 1% of the incident light is absorbed B. 10% of the incident light is transmitted C. 10% of the incident light is absorbed […]