Discover the Pioneers Behind Blotting Techniques and DNA Fingerprinting
39. Arrange the following in correct order
1. Southern Blotting—- A) Edwin
2. Western blotting—- B) E.M.Southern
3. Northern Blotting—- C) A. Jeffrey
4. DNA fingerprinting—- D) Towbin
a) 1-A, 2-C, 3-D, 4-B
b) 1-B, 2-D, 3-A, 4-C
c) 1-B, 2-A, 3-D, 4-C
d) 1-B, 2-C, 3-A, 4-C
Correct Answer: (b) 1-B, 2-D, 3-A, 4-C
Pioneers of Blotting Techniques and DNA Fingerprinting : Understanding the origins of key molecular biology techniques helps appreciate the advancements in genetic research, diagnostics, and forensic science. The correct order of discovery and the scientists behind these methods are as follows:
- Southern Blotting – Edwin M. Southern (B)
- Western Blotting – Towbin (D)
- Northern Blotting – James Alwine, David Kemp, and George Stark (A)
- DNA Fingerprinting – Alec Jeffrey (C)
Explanation
-
Southern Blotting (1975) – Edwin M. Southern
- Southern blotting is a technique used to detect specific DNA sequences in a sample.
- In this method, DNA is digested with restriction enzymes, separated by gel electrophoresis, transferred to a membrane, and hybridized with a labeled probe.
- Southern’s discovery enabled the identification of genetic mutations and DNA fingerprinting applications.
-
Western Blotting (1979) – Towbin
- Western blotting is used for detecting specific proteins in a sample.
- Proteins are separated by SDS-PAGE, transferred to a membrane, and identified using primary and secondary antibodies.
- Western blotting is widely used in protein research, disease diagnosis, and drug development.
-
Northern Blotting (1977) – James Alwine, David Kemp, and George Stark
- Northern blotting is designed for RNA detection.
- RNA is extracted, separated by electrophoresis, transferred to a membrane, and hybridized with a complementary probe.
- It is used for studying gene expression and mRNA levels in various tissues.
-
DNA Fingerprinting (1984) – Alec Jeffrey
- DNA fingerprinting is a method used to identify individuals based on their unique DNA patterns.
- It involves analyzing Variable Number Tandem Repeats (VNTRs) and is widely applied in forensics, paternity testing, and evolutionary biology.
Conclusion
The development of Southern, Western, and Northern blotting techniques, along with DNA fingerprinting, transformed molecular biology. These methods are now essential tools in medical research, genetic analysis, and forensic science. Their discovery has paved the way for breakthroughs in disease diagnostics, gene expression studies, and criminal investigations.
65 Comments
Arushi
April 9, 2025👍✔️
Khushi Vaishnav
August 19, 2025S B – E. M. Southern
W B – Towbin
N B – James
DNA Fingerprinting -Jeffrey
Aakansha Sharma
August 20, 2025Option B
Divya rani
August 20, 2025Southern blotting. ….E.M southern
Northern blotting. …..james alwine, david kemp And george stark
Western blotting. …….towbin
Dna finger printing. ….alec jeffrey
Santosh Saini
August 20, 2025SB – E. M. Southern , WB – Towbin , NB – James, DNA F. P. – A. Jeffrey
Dipti Sharma
August 20, 2025Southern Blotting – Edwin M. Southern
Western Blotting – Towbin
Northern Blotting – James Alwine, David Kemp, and George Stark
DNA Fingerprinting – Alec Jeffrey
Asha Gurzzar
August 20, 2025Southern blotting by em Southern, western blotting by towbin, northern blotting by James ,and dna fingerprint by a .jefferey
Neelam Sharma
August 20, 2025Southern Blotting – Edwin M. Southern
Western Blotting – Towbin
Northern Blotting – James Alwine, David Kemp, and George Stark
DNA Fingerprinting – Alec Jeffrey
Diksha Chhipa
August 20, 2025Information got it sir
anjani sharma
August 20, 2025Answer b matches the correct answers
Khushi Mehra
August 20, 2025Correct option is b
1-B
2-D
3-A
4-C
Priya dhakad
August 20, 2025Southern Blotting – Edwin M. Southern
Western Blotting – Towbin
Northern Blotting – James Alwine, David Kemp, and George Stark
DNA Fingerprinting – Alec Jeffrey
Kavita choudhary
August 20, 2025Answer is b
Priti khandal
August 20, 2025Ok sir
Mohd juber Ali
August 21, 2025Option b
1. Southern blotting – Edwin m.s.
2. Western – towbin
3. Northern – George David and James Alvin
4. Dna fingerprinting – alec
Meenakshi Choudhary
August 21, 2025Option b
Payal Gaur
August 21, 2025Option b
Meera Gurjar
August 21, 2025Option b is correct
Sheikh Aleen
August 21, 2025Southern Blotting – Edwin M. Southern (
Western Blotting – Towbin
Northern Blotting – James Alwine
DNA Fingerprinting – Alec Jeffrey
Manisha choudhary
August 27, 2025DNA fingerprinting – E.M. southern
Western blotting – townin
Northern blotting – Edwin
DNA fingerprinting – alic Jeffrey
Parul Yadav
August 21, 2025Southern Blotting – Edwin M .Southern
Northern Blotting – JAMES ALWINE, David Kemp and Stark
Western Blotting – Towbin
DNA Fingerprinting – Alec Jaffrey
Bharti Yadav
August 21, 2025Southern Blotting – Edwin M. Southern
Western Blotting – Towbin
Northern Blotting – James Alwine, David Kemp, and George Stark
DNA Fingerprinting – Alec Jeffrey
Aafreen Khan
August 21, 2025Option b
1. Southern blotting – Edwin m.s.
2. Western – towbin
3. Northern – George David and James Alvin
4. Dna fingerprinting – alec
shruti sharma
August 22, 2025option b
Aman Choudhary
August 22, 2025Option B is correct
Southern Blotting – E.M. Southern
Western Blotting – Towbin
Northern Blotting – James Alwine
DNA Fingerprinting – A. Jeffrey
Simran Saini
August 22, 2025Option B.
Southern blotting – Edmin M. Southern.
Western blotting – Towbin.
Northern blotting – James Alwin.
DNA fingerprinting – Alec Jeffery.
Priyanka choudhary
August 22, 2025Right answer is option b,
Southern Blotting _ Edwin M. Southern
Western Blotting _ Towbin
Northern Blotting _ James Alwine
DNA Fingerprinting _ Alec Jeffrey
Priyanka choudhary
August 22, 2025Right answer is option b
Southern Blotting _ Edwin M.
Western Blotting _ Towbin
Northern Blotting _ James Alwine
DNA Fingerprinting _ Alec Jeffrey
Karishma
August 22, 2025dna fingerprint- A. jeffrey
southern- E.M southern
northern- james alwine
western- towbin
Kirti agarwal
August 23, 2025Opt b
Soniya Shekhawat
August 23, 2025Southern blotting by edwin M, NORTHERN BLOTTING BY Towin, WESTERN BLOTTING BY JAMES, DNA fingerprint by ALEC jeffery.
Mitali saini
August 23, 2025Correct Answer: (b) 1-B, 2-D, 3-A, 4-C
Varsha Tatla
August 23, 2025Southern blotting -e.m Southern
Western blotting -towbin
Northern blotting -edwin
Dna finger printing -jeffery
Niti Tanwar
August 23, 2025S.B- e.m southern
W.B- towbin
N.B- Edwin
Dna finger print – alec Jeffrey
Akshita Jangid
August 23, 2025Option B is correct Answer
Sakshi Kanwar
August 23, 2025Southern blot – E.M Southern
Western blot – Towbin
Northern blot – Edwin
DNA Fingerprinting – A.Jeffrey
Kajal
August 23, 2025Option b according to which:-
Southern blotting -E.M Southern
Dna finger printing -A.Jeffrey
Northern blotting -Edwin
Western blotting -Towbin
Divya Mandhania
August 23, 2025Southern blotting ~ em southern
DNA fingerprint ~ a jeffery
Northern blotting~ Edwin
Western blotting ~ towbin
Yogita
August 24, 2025Southern blotting – E.M. Southern
Western blotting – Towbin,
Northern blotting – Edwin,
DNA fingerprinting – Alec Jeffrey
Shobha Sharma
August 24, 2025Southern blotting – E.M. southern
Western blotting – Towbin
Northern blotting – Edwin
DNA finger printing – Alec Jeffery
Arushi Saini
August 24, 2025Southern blotting :-EW southern
Northern:- edwin
Western:- towbin
Dna finger printing:- alec jeffery
Sneha Kumawat
August 24, 2025Southern blotting -edwin m. Southern
Western blotting-towin
Northern blotting – james alwine , david kemp ,
Dna fingerprinting -alec jeffery
AKANKSHA RAJPUT
August 24, 2025Southern Blotting – (B) Edwin M. Southern
Western Blotting – (D)Towbin
Northern Blotting – (A)James Alwine, David Kemp, and George Stark
DNA Fingerprinting – (C)Alec Jeffrey
Shivani
August 24, 2025Southern Blotting – Edwin M. Southern
Western Blotting – Towbin
Northern Blotting – James Alwine, David Kemp, and George Stark
DNA Fingerprinting – Alec Jeffrey
Anisha Beniwal
August 24, 2025Option B
Devika
August 24, 2025B,d,a,c
Himanshi
August 24, 20251-B, 2-D, 3-A, 4-C
Pooja Sharma
August 24, 2025OPTION B IS CORRECT
Priyanka Verma
August 24, 2025Southern Blotting – Edwin M. Southern
Western Blotting – Towbin
Northern Blotting – James Alwine, David Kemp, and George Stark
DNA Fingerprinting – Alec Jeffrey
Sonam Saini
August 24, 2025Southern bloating _edwin m Southern
Western bloating _towbin
Northern blotting James Alvin David kemp and george
DNA fingerprinting _alec Jeffrey
Falguni Prakash
August 24, 2025B
Swati Choudhary
August 24, 2025Southern blotting -edwin m. Southern
Western blotting-towin
Northern blotting – james alwine , david kemp ,
Dna fingerprinting -alec jeffery
Ajay Sharma
August 25, 2025option b
Kashish yadav
August 25, 2025Done sir
Kajal
August 25, 2025Option – B, D,A,C
Bhavana kankhedia
August 26, 20251-B, 2-D, 3-A, 4-C
Priyanka Meena
August 26, 2025B correct
Muskan Yadav
August 26, 2025Southern Blotting – Edwin M .Southern
Northern Blotting – JAMES ALWINE, David Kemp and Stark
Western Blotting – Towbin
DNA Fingerprinting – Alec Jaffrey
Kanica Sunwalka
August 28, 2025done
Komal Sharma
August 28, 2025Option two is correct
Shriyanshi verma
August 30, 2025SB – E. M. Southern , WB – Towbin , NB – James, DNA F. P. – A. Jeffrey
Pratibha Jain
August 30, 2025Correct Answer is option (b)
1-B, 2-D, 3-A, 4-C
Alec
August 30, 2025correct ans. is option b – 1-B, 2-D, 3-A, 4-C
Anisha jakhar
August 31, 2025B is the correct option
Shobha Kanwar
August 31, 2025Option B is correct SB – E. M. Southern , WB – Towbin , NB – James, DNA F. P. – A. Jeffrey