Gel Electrophoresis: The Key Technique for Separating Charged Molecules

Gel Electrophoresis: The Key Technique for Separating Charged Molecules

Separation of charged molecules are separated based on
varying rates of migration through a solid matrix when
subjected to an electric field
A. Photoreactivation
B. Gel electrophoresis
C. Autoradiography
D. Eastern blotting

Gel Electrophoresis: A Powerful Technique for Molecular Separation

Gel electrophoresis is a widely used technique in molecular biology and biochemistry for separating charged molecules, such as DNA, RNA, and proteins, based on their migration through a gel matrix when subjected to an electric field. The correct answer to the question is:

B. Gel Electrophoresis

This technique is essential for analyzing genetic material, protein expression, and molecular interactions.

Principle of Gel Electrophoresis

Gel electrophoresis works by applying an electric current to a gel, which creates an electric field. Molecules move through the gel at different rates depending on their size and charge. Negatively charged molecules, like DNA and RNA, migrate toward the positive electrode (anode), while proteins may require SDS (sodium dodecyl sulfate) treatment to impart a uniform negative charge.

Steps in Gel Electrophoresis

  1. Preparation of the Gel

    • Agarose gel is used for DNA and RNA separation.
    • Polyacrylamide gel is used for proteins and small nucleic acids.
  2. Loading the Sample

    • DNA or proteins are mixed with a loading dye and placed into wells in the gel.
  3. Application of Electric Field

    • The gel is submerged in a buffer solution, and an electric current is applied.
  4. Molecular Separation

    • Smaller molecules travel faster and farther, while larger molecules migrate slower.
  5. Visualization

    • DNA is stained with ethidium bromide or SYBR Green and viewed under UV light.
    • Proteins are stained with Coomassie Blue or Silver Stain.

Why Are the Other Options Incorrect?

A. Photoreactivation – A DNA repair mechanism, not a separation technique.

C. Autoradiography – A detection method for radioactively labeled molecules.

D. Eastern Blotting – Used for studying protein modifications, not for molecular separation.

Conclusion

Gel electrophoresis is a fundamental technique for biological and forensic research, allowing scientists to analyze genetic material, identify mutations, and study proteins.

 

82 Comments
  • Suman bhakar
    March 8, 2025

    ☑️☑️

    • Mahima Sharma
      August 21, 2025

      Gel electrophoresis

  • Kabeer Narwal
    March 18, 2025

    👍👍

  • Akshita
    August 14, 2025

    Correct answer B
    Gel electrophoresis

  • Simran Saini
    August 17, 2025

    Correct answer B
    Gel electrophoresis

  • Khushi Vaishnav
    August 17, 2025

    Gel Electrophoresis

  • Meenakshi Choudhary
    August 18, 2025

    Gel-electrophoresis

  • Swati
    August 19, 2025

    Gel Electrophoresis

  • Aakansha Sharma
    August 20, 2025

    Gel electrophoresis

  • Dipti Sharma
    August 20, 2025

    Gel electrophoresis is separation of charged particles on the basis of their mw.

  • Khushi Mehra
    August 20, 2025

    Gel electrophoresis is used for separation of charged particles or molecules on the basis of molecular weight by applying electric field.

  • shruti sharma
    August 20, 2025

    gel electrophoresis

  • Diksha Chhipa
    August 20, 2025

    Done sir

  • Anurag Giri
    August 20, 2025

    Gel electrophoresis is a widely used teq for separating charged molecules

  • Dharmpal Swami
    August 20, 2025

    Gel electrophoresis -seperating charge molecules

  • Nilofar Khan
    August 20, 2025

    Gel electrophoresis

  • Bharti Yadav
    August 20, 2025

    Gel electrophoresis

  • Sakshi yadav
    August 20, 2025

    Gel electrophoresis

  • Surbhi Rajawat
    August 20, 2025

    Gel electrophoresis is the technique to separate or differentiate the DNA , RNA and protein on the basis of their molecular weight.

  • Priya dhakad
    August 20, 2025

    Gel electrophoresis technique is used to separate charge particle.

  • anjani sharma
    August 20, 2025

    Answer b gel electrophoresis

  • Varsha Tatla
    August 20, 2025

    Gel electrophoresis

  • Kavita choudhary
    August 20, 2025

    Gel electrophoresis is a technique to seprate the charge partical

  • Neelam Sharma
    August 20, 2025

    Gel electrophoresis

  • Deepika Sheoran
    August 20, 2025

    Gel electrophoresis techniques used to saprate charge particles .

  • Neha
    August 21, 2025

    Gel electrophoresis

  • Payal Gaur
    August 21, 2025

    Gel electrophoresis seprate charge molecules/particles

  • Sneha .......
    August 21, 2025

    Gel electrophoresis

  • Mohd juber Ali
    August 21, 2025

    (b) gel electrophoresis

  • Komal Sharma
    August 21, 2025

    Gel is a separation technique based on electric current to a gel, which creates an electric field. Molecules move through the gel at different rates depending on their size and charge. Negatively charged molecules, like DNA and RNA, migrate toward the positive electrode (anode), while proteins may require SDS (sodium dodecyl sulfate) treatment to impart a uniform negative charge.

  • Aman Choudhary
    August 21, 2025

    Gel electrophoresis is technique used in separation of charge molecules

  • Manisha
    August 21, 2025

    Done with explanation ✅

  • Aafreen Khan
    August 21, 2025

    Gel electrophoresis works apply an electric current to which creates an electric field. Molecules move through the gel at different rates depending on their size and charge

  • Avni
    August 21, 2025

    Gel electrophoresis

  • Simran Saini
    August 21, 2025

    Gel electrophoresis.

  • Bhawna Choudhary
    August 21, 2025

    Gel electrophoresis

  • Santosh Saini
    August 21, 2025

    Gel electrophoresis is a technique for separation of charge molecules based on electric field

  • Sakshi Kanwar
    August 22, 2025

    B separation of charges molecule on the basis of molecular weight

  • Priyanka Choudhary
    August 22, 2025

    Correct answer is gel electrophoresis is separation of charged particles on the basis of the molecular weight

    Reply

  • Soniya Shekhawat
    August 22, 2025

    Gel electrophoresis.

  • karishma
    August 22, 2025

    Separation of charged molecules are separated based on varying rates of migration through a solid matrix when subjected to an electric field is gel electrophoresis

  • Arushi
    August 22, 2025

    Gel electrophoresis

  • Kirti agarwal
    August 23, 2025

    Gel electrophoresis

  • Priya khandal
    August 23, 2025

    Gel electrophoresis

  • Niti Tanwar
    August 23, 2025

    Gel electrophoresis

  • Akshita Jangid
    August 23, 2025

    Gel electrophoresis is a technique used to separate the charge particles under the influence of electric field

  • Kajal
    August 23, 2025

    Gel electrophoresis is the correct one.

  • Devika
    August 24, 2025

    Gel electrophoresis separate charged molecules in basis of molecular weight

  • Akshita Jangid
    August 24, 2025

    Gel electrophoresis is a technique used for separation of charge molecules based on electric field

  • Divya Mandhania
    August 24, 2025

    Gel electrophoresis is used for separation of charged particles or molecules on the basis of molecular weight by applying electric field.

  • Sneha Kumawat
    August 24, 2025

    Gel electrophoresis is used for separation of charge particles or molecules on the basis of molecular weight

  • Yogita
    August 24, 2025

    Gel electrophoresis

  • Urmi sisodiya
    August 24, 2025

    Gel electrophoresis is correct bcoz it is the technique which seperate the molecules in the basis of charge by providing electric field

  • Ankita Pareek
    August 24, 2025

    Gel electrophoresis is a technique where changed molecules are subjected to electric field for separation

  • AKANKSHA RAJPUT
    August 24, 2025

    DNA, RNA, proteins, based on their molecular weight seprate on a gel on the basis of charge is called gel electrophoresis .

  • Shivani
    August 24, 2025

    Option B. – gel electrophoresis

  • Bhavana kankhedia
    August 24, 2025

    Gel Electrophoresis

  • Neeraj Sharma
    August 24, 2025

    Gel electrophoresis as it can separate on the basis of charge and via charge

  • Anisha Beniwal
    August 24, 2025

    Option B

  • Meera Gurjar
    August 24, 2025

    Gel electrophoresis

  • Ayush Dubey
    August 24, 2025

    Gel Electrophoresis

  • Himanshi
    August 24, 2025

    Gel electrophoresis

  • Tanvi Panwar
    August 24, 2025

    Gel electrophoresis is a technique for the separation of charged molecules in the presence of electric field.

  • Priyanka Verma
    August 24, 2025

    correct answer is Gel electrophoresis

  • Sonam Saini
    August 24, 2025

    Gel electrophoresis

  • Surendra Doodi
    August 24, 2025

    Gel Electrophoresis

  • Falguni Prakash
    August 24, 2025

    Gel electrophoresis

  • Ajay Sharma
    August 25, 2025

    gel electrophoresis

  • Kajal
    August 25, 2025

    Gel electrophoresis

  • Priyanka Meena
    August 26, 2025

    Gel electrophoresis

  • Muskan Yadav
    August 26, 2025

    Gel electrophoresis – technique is essential for analyzing genetic material, protein expression, and molecular interactions.

  • Manisha choudhary
    August 27, 2025

    Gel electrophoresis correct answer
    Estern blotting protein modification identify technique h
    Autoradiography, radioactivity labelled molecule detection technique h
    Photo reactivation DNA repair technique h

  • Savita Garwa
    August 27, 2025

    Gel electrophoresis is correct bcoz it is the technique which seperate the molecules in the basis of charge by providing electric field

  • Kanica Sunwalka
    August 28, 2025

    gel electrophoresis

  • Shriyanshi verma
    August 30, 2025

    Correct answer B
    Gel electrophoresis

  • Asha Gurzzar
    August 30, 2025

    Gel electrophoresis is correct answer

  • Alec
    August 30, 2025

    option b is correct gel electrophoresis for separating charged molecules

  • Pratibha Jain
    August 30, 2025

    The correct answer is option (B)
    Gel Electrophoresis

  • Anisha jakhar
    August 31, 2025

    Gel electrophoresis

  • Shobha Kanwar
    August 31, 2025

    Gel electrophoresis is separation of charged particles on the basis of their molecular weight

  • Ajay Sharma
    September 2, 2025

    It’s gel electrophoresis, charged particles move towards opposite charged electrode according to their mw

  • Konika
    September 10, 2025

    Gel electrophoresis

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