Gel Electrophoresis: A Powerful Technique for Molecular Separation
Gel electrophoresis is a widely used technique in molecular biology and biochemistry for separating charged molecules, such as DNA, RNA, and proteins, based on their migration through a gel matrix when subjected to an electric field. The correct answer to the question is:
✅ B. Gel Electrophoresis
This technique is essential for analyzing genetic material, protein expression, and molecular interactions.
Principle of Gel Electrophoresis
Gel electrophoresis works by applying an electric current to a gel, which creates an electric field. Molecules move through the gel at different rates depending on their size and charge. Negatively charged molecules, like DNA and RNA, migrate toward the positive electrode (anode), while proteins may require SDS (sodium dodecyl sulfate) treatment to impart a uniform negative charge.
Steps in Gel Electrophoresis
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Preparation of the Gel
- Agarose gel is used for DNA and RNA separation.
- Polyacrylamide gel is used for proteins and small nucleic acids.
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Loading the Sample
- DNA or proteins are mixed with a loading dye and placed into wells in the gel.
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Application of Electric Field
- The gel is submerged in a buffer solution, and an electric current is applied.
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Molecular Separation
- Smaller molecules travel faster and farther, while larger molecules migrate slower.
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Visualization
- DNA is stained with ethidium bromide or SYBR Green and viewed under UV light.
- Proteins are stained with Coomassie Blue or Silver Stain.
Why Are the Other Options Incorrect?
❌ A. Photoreactivation – A DNA repair mechanism, not a separation technique.
❌ C. Autoradiography – A detection method for radioactively labeled molecules.
❌ D. Eastern Blotting – Used for studying protein modifications, not for molecular separation.
Conclusion
Gel electrophoresis is a fundamental technique for biological and forensic research, allowing scientists to analyze genetic material, identify mutations, and study proteins.
82 Comments
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Akshita
August 14, 2025Correct answer B
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August 17, 2025Correct answer B
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Anurag Giri
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anjani sharma
August 20, 2025Answer b gel electrophoresis
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Mohd juber Ali
August 21, 2025(b) gel electrophoresis
Komal Sharma
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Manisha
August 21, 2025Done with explanation ✅
Aafreen Khan
August 21, 2025Gel electrophoresis works apply an electric current to which creates an electric field. Molecules move through the gel at different rates depending on their size and charge
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Sakshi Kanwar
August 22, 2025B separation of charges molecule on the basis of molecular weight
Priyanka Choudhary
August 22, 2025Correct answer is gel electrophoresis is separation of charged particles on the basis of the molecular weight
Reply
Soniya Shekhawat
August 22, 2025Gel electrophoresis.
karishma
August 22, 2025Separation of charged molecules are separated based on varying rates of migration through a solid matrix when subjected to an electric field is gel electrophoresis
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Ankita Pareek
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AKANKSHA RAJPUT
August 24, 2025DNA, RNA, proteins, based on their molecular weight seprate on a gel on the basis of charge is called gel electrophoresis .
Shivani
August 24, 2025Option B. – gel electrophoresis
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Anisha Beniwal
August 24, 2025Option B
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Manisha choudhary
August 27, 2025Gel electrophoresis correct answer
Estern blotting protein modification identify technique h
Autoradiography, radioactivity labelled molecule detection technique h
Photo reactivation DNA repair technique h
Savita Garwa
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Shriyanshi verma
August 30, 2025Correct answer B
Gel electrophoresis
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Alec
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Pratibha Jain
August 30, 2025The correct answer is option (B)
Gel Electrophoresis
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Shobha Kanwar
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Ajay Sharma
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