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81. A novel enzyme was Identified in humans. The followingapproaches are available to identify the chromosome on which the gene encoding the enzyme is present: (A) Suppress the activity of […]

80 To detect mutation (GAGàGTG) allele specific hybridization method is used. Four members of an affected family are investigated. DNA isolated from blood samples of parents and two offsprings are […]

79.interspecific somatic cell hybrids have proved very useful for (1) Gene mapping    (2) Genetic manipulation (3) Gene structure   (4) Gene function Interspecific somatic cell hybrids, formed by fusing somatic cells […]

78 ‘TaqMan’ assay for detection of base substitutions (DNA variants).probes (oligonucleotides) with fluorescent dyes at the 5’-end and quencher at 3’-end are used. While the probe is intact, the proximity […]

77. The pedigree given below represents the genotype at four different loci for the children in generation III. Which one of the given genotypes is likely to represent the genotype […]

76. In the following pedigree three STR loci A, B and C are linked on the long arm of the X-chromosome in the order centromere-A-B-C-telomere. Further in the table, the […]

75. Polymorphic DNA sequences are used for molecular identification. Short tandem repeats (STRS) and Single Nucleotide Polymorphism (SNPs) are used as polymorphic markers. The table below summarizes the status of […]

The following represents sequences of different alleles of a gene found in family represented by mother, (allele1/ allele2), father (allele1/allele 2) and their two sons: Son1 (allele1/allele 2) and Son […]

73. Microsatellite are used as marker for identifying individuals via DNA fingerprinting os the alleles may differ in the number of repeals. From the Southern blot shown below identify the […]

72. The pedigree given below follows the inheritance pattern of a late-onset (after age of 30 years) genetic disease that is 100% penetrant. Affected individuals are indicated by a solid […]