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19. Cre/loxP system is used by phage Pl to remove terminally redundant sequences that arise during packaging of the phage DNA. Cre-lox system can be used to create targeted deletions, […]

18. Site specific recombination results in precise DNA rearrangements, which is limited to specific sequences. The enzymes that are important to carry out the process are (1) Restriction endonuclease and […]

17. In order to create an animal whose prostate gland is devoid of the p53 gene, engineered animals were created by cloning specially designed knock-out cassettes. Identify the most appropriate […]

In a transgenic mice line, lox P sites are introduced in the target A in the following manner This transgenic mice line was mated with another transgenic mice line where […]

15. A student was asked to design a knockout cassette for specifically deleting the p53 gene from the prostate gland of mice. Which one of the following pairs of cassettes […]

14. In transgenic mice, the orientation an location of the loxP sites determine whether Cre recombinase induces a deletion, an inversion or a chromosomal translocation. If a researcher wants to […]

13. Which one of the following statements about Cre- mediated site-specific recombination at loxP sites is INCORRECT? (1) When loxP sites flanking a test sequence are oriented in same direction, […]

12. In the following diagram, segments A and C are copies of 10 base pair repeat DNA sequences, flanking a unique stretch shown as B, A and C are in […]

11. The following cassette was designed to create estrogen receptor knock-out mice: SoH: site of homology; Gol: gene of interest What would ensure that proper recombination has taken place? (1) […]

10. A student while constructing knock-out mice isolated mouse embryonic, stem cells and introduced an engineered DNA into the cells. However, none of the mice were transgenic. On checking the […]