If the amount of agarose added is more, the molecule under analysis should have following characteristic

If the amount of agarose added is more, the molecule under analysis should have following characteristic

(a) Small size
(b) Large size
(c) Size has no relation with amount of agarose
(d) Amount of molecules under analysis matters

 

Correct answer: (A) Small size


Explanation:

Higher agarose concentrationSmaller poresBetter resolution for smaller molecules

  • Lower agarose concentrationLarger poresBetter resolution for larger molecules

Thus, if more agarose is added, the gel becomes denser, making it easier for small molecules to pass through, while larger molecules get trapped and move slower.


Why Not the Other Options?

  • (B) Large size

    • Large molecules migrate poorly in high-concentration agarose gels because of small pore size.
  • (C) Size has no relation with amount of agarose

    • Size is directly related to agarose concentration. More agarose = better resolution of smaller molecules, while lower agarose = better resolution of larger molecules.
  • (D) Amount of molecules under analysis matters

    • The quantity of sample affects band intensity, but not the resolution or separation efficiency.

Final Answer:

(A) Small size

60 Comments
  • Prami Masih
    February 27, 2025

    Okay sir

    • Mahima Sharma
      August 24, 2025

      Small size

  • Arushi
    April 1, 2025

    👍✔️

  • Anita choudhary
    April 19, 2025

    ☑️👍

  • Anurag Giri
    August 13, 2025

    Ans 1 Higher agarose concentration → Smaller pores → Better resolution for smaller molecules

  • Khushi Agarwal
    August 13, 2025

    Option 1 is correct
    If concentration increase then pore size decrease

  • Aman Choudhary
    August 13, 2025

    option a is correct
    because agarose does not have any crosslinker to reduce pore size if more addition of agarose the size become decrease

  • Soniya Shekhawat
    August 13, 2025

    Concentration is inversely proportional to pore size

  • Soniya Shekhawat
    August 13, 2025

    So small size is correct option.

  • Mohd juber Ali
    August 14, 2025

    Option (a) Agarose gel ki concentration increase karne par pore size kam ho jate h only small molecule pass ho sakta h large molecule tarp ho jate h pore size kam hone se molecule small size…

  • Anjani sharma
    August 14, 2025

    Answer 1 as the agarose concentration increase the pore size will decrease

  • Mitali
    August 14, 2025

    (A) Small size

    • Priya dhakad
      August 14, 2025

      Answer 1st is correct if agarose concentration is increased then pore size small

  • Karishma Don
    August 14, 2025

    increase concentration of agarose leads to small pores

  • Nilofar Khan
    August 14, 2025

    Correct answer is a

  • Mansukh Kapoor
    August 14, 2025

    The correct answer is option 1st
    Small size because of more agarose is added than it becomes denser and it is easier to pass small molecules and large molecules trapped in it

    • Priyanka choudhary
      August 14, 2025

      Correct answer the small size because of this the consentration is increase than pore size decrease

  • Sneha Kumawat
    August 14, 2025

    Higher agro concentration hone pores size small ho jata hai jo small molecules ke liye better resolution deta hai

  • Roopal Sharma
    August 14, 2025

    Small size

  • Dipti Sharma
    August 14, 2025

    Higher agarose concentration means Smaller pores which gives better resolution for smaller molecules.

  • Deepika Sheoran
    August 14, 2025

    Option A is correct
    Small size Agrose construction increase the pore size will decrease.

  • Monika jangid
    August 14, 2025

    High agarose = small pores

  • Neelam Sharma
    August 14, 2025

    Small size

  • Priti khandal
    August 14, 2025

    Option A is right sir small size because Agarose concertration is increase then pore size small

  • Priya khandal
    August 14, 2025

    Small size

  • shruti sharma
    August 15, 2025

    small size

  • Divya rani
    August 16, 2025

    Small size kyunki Agarose ki jyada concentration s chote pore bnenge yani chote molecules usme separate jyada Ache s honge

  • Aafreen
    August 17, 2025

    Ans -1 small size
    If more agarose is added the gel becomes denser it makes easier for small molecules to pass through it

  • Kavita choudhary
    August 17, 2025

    Small size

  • Avni
    August 17, 2025

    Small size
    High agarose concentration → small pores

  • Neeraj Sharma
    August 18, 2025

    Option A small size is correct as the concentration is inversely proportional to pore size so the larger the agarose molecules more binding pore size less hence the molecule must be small to move easily and for better resolution

  • Bharti Yadav
    August 24, 2025

    Higher agarose concentration → Smaller pores
    Lower agarose concentration → Larger pores

  • Varsha Tatla
    August 24, 2025

    Conc.1/pore size
    It means molecules size will small

  • Priyanka Choudhary
    August 24, 2025

    Small size (a) ,is right answer because of this,
    Higher agarose concentration → Smaller pores → Better resolution for smaller molecules

  • Anil Choudhary
    August 24, 2025

    Small

  • Bhavana kankhedia
    August 24, 2025

    small size

  • Sonam Saini
    August 24, 2025

    स्मॉल साइज

  • Alec
    August 24, 2025

    (a) small size because increasing the agarose results in smaller pore size in the gel so smaller sized molecules are able to pass.

  • Niti Tanwar
    August 24, 2025

    Small size

  • Aartii sharma
    August 24, 2025

    Smaller size

  • Anjali
    August 24, 2025

    Small size is correct

  • Anju
    August 24, 2025

    Ans : a
    As the agarose conc increase the pour side decrease and this impoves the resolution

  • Falguni Prakash
    August 24, 2025

    High agrose mean small pores

  • Kashish yadav
    August 25, 2025

    Small size is right answer

  • Ayush Dubey
    August 25, 2025

    Small size

  • sakshi khatri
    August 25, 2025

    done

  • Surendra Doodi
    August 28, 2025

    Higher agarose concentration → Smaller pores → Better resolution for smaller molecules

  • Pratibha Jain
    August 30, 2025

    Correct answer is option (A)
    Small size
    Higher agarose concentration → Smaller pores → Better resolution for smaller molecules
    Lower agarose concentration → Larger pores → Better resolution for larger molecules

  • Shriyanshi verma
    August 30, 2025

    Option 1 is correct
    If concentration increase then pore size decrease

  • Sheikh Aleen
    August 30, 2025

    Small size is right ans

  • Patel Radha
    August 30, 2025

    (A) Small size

  • Anisha Beniwal
    August 31, 2025

    Small size

  • Vanshika Sharma
    August 31, 2025

    If concentration increases then pore size decreases

  • Shobha Kanwar
    August 31, 2025

    Option 1 is correct If concentration increase then pore size decrease

  • Aartii sharma
    September 1, 2025

    The molecules should be of small size

  • Anisha jakhar
    September 3, 2025

    Correct option is A. Small size

  • Muskan Yadav
    September 3, 2025

    Higher agarose concentration → Smaller pores → Better resolution for smaller molecules

  • Somya Yadav
    September 5, 2025

    Small size

  • Meera Gurjar
    September 13, 2025

    Small size

  • Aakansha sharma Sharma
    October 1, 2025

    Correct answer: (A) Small size

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