How is the size of molecules under analysis measured

How is the size of molecules under analysis measured

(a) By measuring the distance moved through a ruler
(b) By measuring the amount of visualizing dye used
(c) By running a standard molecule whose size is known in parallel
(d) There is no exact criteria for doing so

 

Correct answer: (C) By running a standard molecule whose size is known in parallel


Explanation:

To determine the size of molecules (e.g., DNA, RNA, or proteins) during electrophoresis, a standard molecular marker (also called a ladder) is run alongside the sample.

  • The marker contains fragments of known sizes, allowing for comparison with the unknown sample.
  • By comparing the migration distance of the sample with the standard, the size of the unknown molecule can be estimated.

Why Not the Other Options?

  • (A) By measuring the distance moved through a ruler

    • Distance alone does not determine size because migration depends on multiple factors, including gel concentration and voltage.
    • A standard ladder is necessary for accurate size determination.
  • (B) By measuring the amount of visualizing dye used

    • Dye intensity relates to the amount of sample present, not the size of the molecule.
  • (D) There is no exact criteria for doing so

    • There is a standard method—using a molecular weight marker or ladder for comparison.

Final Answer:

(C) By running a standard molecule whose size is known in parallel

69 Comments
  • Akshay mahawar
    February 25, 2025

    Doubt

    • Mahima Sharma
      August 24, 2025

      Ans c molecule size known

  • Nisha
    February 25, 2025

    Complete

  • Suman bhakar
    February 26, 2025

    My answer not correct

  • Arushi
    February 26, 2025

    Answer c☑️

  • Parul
    February 27, 2025

    Easily understood. Done sir

  • Pooja jat
    February 27, 2025

    Done.

  • Lavanya Sharma
    February 27, 2025

  • Prami Masih
    February 27, 2025

    👍👍👍

  • yogesh sharma
    February 28, 2025

    Done sir

  • Lokesh kumawat
    March 1, 2025

    Done

  • Abhilasha
    March 1, 2025

    Done sir

  • VIKRAM GAHLOT
    March 7, 2025

    👍

  • Anita choudhary
    April 19, 2025

  • Anurag Giri
    August 13, 2025

    Ans 3 To determine the size of molecules during electrophoresis, a standard molecular marker (also called a ladder) is run alongside the sample.

  • Khushi Agarwal
    August 13, 2025

    Size of molecules analyse measure by running a std molecule whose size is known in parallel that is called ladder

  • Aman Choudhary
    August 13, 2025

    option c is correct bcz of ladder

  • Soniya Shekhawat
    August 13, 2025

    Ladder sequence have running a standard molecule that is whose size is parallel is used to detect unknown molecules so answer 3th is correct.

  • Anjani sharma
    August 14, 2025

    Answer 3 ,there is a ladder of known sequence which is used to detect unknown samples

  • Mitali
    August 14, 2025

    (C) By running a standard molecule whose size is known in parallel

  • Priya dhakad
    August 14, 2025

    By running a standard molecule whose size is known in parallel.

  • Mansukh Kapoor
    August 14, 2025

    The correct answer is option 3rd
    To determine the size of the band during the electrophoresis we to run a standard band (ladder)parallel to our sample

  • Karishma Don
    August 14, 2025

    the ladder or standard molecule is run parallel to the unknown sample and by comparison with unknown sample

  • Sneha Kumawat
    August 14, 2025

    The correct answer is C by running a standard molecule whose size is known in parallel

  • Roopal Sharma
    August 14, 2025

    Ladder isd used

  • Dipti Sharma
    August 14, 2025

    C) By running a standard molecule whose size is known in parallel called ladder.

  • Deepika Sheoran
    August 14, 2025

    Option c is correct
    Because by running a standard molecule whose size is known in parellel is called ladder. Detect unknown molecules.

  • Neelam Sharma
    August 14, 2025

    running a standard molecule whose size is known in parallel

  • Monika jangid
    August 14, 2025

    By running a standard molecule whose size is parallel known as ladder

  • Priyanka choudhary
    August 14, 2025

    Correct answer is option 3rd

  • Priti khandal
    August 14, 2025

    Option C is right by running a standard molecule whose size is known in parallel

  • Priya khandal
    August 14, 2025

    Bye running a standard molecular whose size is know in pareller

  • shruti sharma
    August 15, 2025

    correct ans is c

  • Shivani
    August 15, 2025

    Ans.- C
    By running a standard molecule whose size is known .

    • Aafreen
      August 17, 2025

      Ans -3
      By running a standard molecule whose size is known in parallel

  • Divya rani
    August 16, 2025

    Yes correct answer is C because unknown molecules k size ko identify krne k liye ek known size ka molecule unknown k parallel m chahiye jisko ladder bolenge

  • Manisha gujar
    August 17, 2025

    C option is correct known sample is running from ladder in parellel

  • Manisha gujar
    August 17, 2025

    Option c is correct

  • Kajal
    August 17, 2025

    Option c is right
    By running standard molecule whose size is known in parallel

  • Avni
    August 17, 2025

    correct option is (C)

  • Simran Saini
    August 18, 2025

    Option c.
    By running a standard molecule whose size is known in parallel.

  • Neeraj Sharma
    August 18, 2025

    Option C by adding or running known molecular weight molecule in the parallel so the molecule size is estimated via relative distance

  • Bharti Yadav
    August 24, 2025

    Size of molecules are measured by running known molecules in parallel.

  • Priyanka Choudhary
    August 24, 2025

    Option c is correct answer,
    By running a standard molecule whose size is known in parallel

  • Bhavana kankhedia
    August 24, 2025

    By running a standard molecule whose size is known in parallel

  • Sonam Saini
    August 24, 2025

    By running standard models of known size in parallel to generate small size

  • Alec
    August 24, 2025

    option c – by running a standard molecule of known size in parallel.

  • Aartii sharma
    August 24, 2025

    By running a standard molecule of known size in parallel

  • Anjali
    August 24, 2025

    Ans c is correct

  • Anju
    August 24, 2025

    Ans : c
    Ladder is use because its size is already known to us

  • Falguni Prakash
    August 24, 2025

    C

  • Kashish yadav
    August 25, 2025

    Ok sir

  • Ayush Dubey
    August 25, 2025

    By running a standard molecule whose size is known in parallel

  • sakshi khatri
    August 25, 2025

    done

  • Pratibha Jain
    August 30, 2025

    Correct answer is option (C)
    By running a standard molecule whose size is known in parallel

  • Shriyanshi verma
    August 30, 2025

    Size of molecules analyse measure by running a std molecule whose size is known in parallel that is called ladder

  • Sheikh Aleen
    August 30, 2025

    By running a standard molecule whose size is known in parallel

  • Patel Radha
    August 30, 2025

    By running a standard molecule whose size is parallel known as ladder

  • Anisha Beniwal
    August 31, 2025

    Option C is correct -By running a standard molecule whose size is known in parallel

  • Vanshika Sharma
    August 31, 2025

    By running a standard molecule whose size is known in parallel

  • Komal Sharma
    August 31, 2025

    To determine the size of molecules (e.g., DNA, RNA, or proteins) during electrophoresis, a standard molecular marker (also called a ladder) is run alongside the sample.

  • Shobha Kanwar
    August 31, 2025

    Answer 3 ,there is a ladder of known sequence which is used to detect unknown samples

  • Aartii sharma
    September 1, 2025

    By running a standard molecule whose size is known in parallel

  • Anisha jakhar
    September 3, 2025

    Correct answer is (C) By running a standard molecule whose size is known in parallel

  • Muskan Yadav
    September 3, 2025

    By running a standard molecule whose size is known in parallel

  • Somya Yadav
    September 5, 2025

    By running a known standard molecule with known in parallel

  • Ajay Sharma
    September 6, 2025

    By running a standard molecule whose size is known in parallel

  • Meera Gurjar
    September 13, 2025

    By running a standard molecule whose size is known in parallel

  • Aakansha sharma Sharma
    October 1, 2025

    Correct answer: (C) By running a standard molecule whose size is known in parallel

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