What is an issue with using 2D-PAGE?
a. Hydrophobic proteins may not run as expected due to the hydrophobic surfaces.
b. Some proteins may not migrate through polyacrylamide and therefore not be represented on the gel.
c. Rare cellular proteins are hard to visualize with Coomassie blue protein stain.
d. All of the above are issues with 2D-PAGE
The correct answer is:
(d) All of the above are issues with 2D-PAGE.
Explanation:
2D-PAGE (Two-Dimensional Polyacrylamide Gel Electrophoresis) is a powerful technique for separating complex protein mixtures based on both isoelectric point (pI) and molecular weight. However, it has several limitations:
-
(a) Hydrophobic proteins may not run as expected due to the hydrophobic surfaces
- Membrane proteins are often hydrophobic and tend to precipitate or aggregate instead of properly migrating through the gel.
- This makes them difficult to resolve in 2D-PAGE.
-
(b) Some proteins may not migrate through polyacrylamide and therefore not be represented on the gel
- Very large proteins or proteins with abnormal structures may not enter or migrate properly through the gel.
- This can result in incomplete representation of the sample.
-
(c) Rare cellular proteins are hard to visualize with Coomassie blue protein stain
- Coomassie blue has a low sensitivity, meaning that low-abundance proteins may not be detectable.
- More sensitive stains (e.g., silver staining, SYPRO Ruby, or fluorescent dyes) are needed to visualize rare proteins.
Conclusion:
Since all the issues listed affect 2D-PAGE, the correct answer is:
✅ (d) All of the above are issues with 2D-PAGE.
69 Comments
Akshay mahawar
February 25, 2025Done👍
Suman bhakar
February 26, 2025Done
Arushi
February 26, 2025☑️👍
Parul
February 27, 2025Understood by explanation and video. Thankyou sir
Pooja jat
February 27, 2025Done.
Lavanya Sharma
February 27, 2025✅👍🏻
Prami Masih
February 27, 2025Done sir
yogesh sharma
February 28, 2025Done sir
Lokesh kumawat
March 1, 2025Done
Abhilasha
March 1, 2025Done sir
Nisha
March 1, 2025Interesting questions
Ashwini shekhawat
March 2, 2025Done
Priya khandal
August 14, 20254 is right
VIKRAM GAHLOT
March 7, 2025👍
Anita choudhary
April 18, 2025✅👍
Aman Choudhary
August 13, 2025option d is correct
Pore size less hone ki wajah se PAGE me large size proteins can’t run
CBB dye is low sensitive do not stain small size protein
Mohd juber Ali
August 14, 2025Option (4) ✅
shruti sharma
August 14, 2025option d iscorrect
Dharmpal Swami
August 14, 20252D- PAGE separate the protein complex based on -1.charge
2.mass
Priya dhakad
August 14, 2025Option d is correct
2D Gel separate the protein based upon charge and mass
Soniya Shekhawat
August 14, 2025Here explanation and vedio solution both are in high quality of knowledgeable about topic , this website is best for csirnet aspirants 😊 Thanks sir for this🙏🏻
Anjani sharma
August 14, 2025Answer d ,2D separate on the basis of charge and size
Neelam Sharma
August 14, 2025All of the above
Mansukh Kapoor
August 14, 2025The correct answer is option 4th because 2D PAGE separates on the basis of charge and molecular size of the protein
Aafreen
August 14, 2025Ans -4
All of the above are issues with 2D-PAGE
Dipti Sharma
August 14, 2025correct answer is:
(d) All of the above are issues with 2D-PAGE.
Karishma
August 14, 2025all of the above
Priti khandal
August 14, 2025the correct answer ke d
Komal Pareek
August 14, 2025All
As visualization is not clear proteins aggregate and also some protein size is more
Deepika Sheoran
August 15, 2025Option d is correct answer
2D gel saprate the protein based upon charge &Mass.
Shivani
August 15, 2025All of the above
Payal
August 17, 2025Option D all of the above are issue in 2 D gel technique
Manisha choudhary
August 17, 2025Membrane proteins mostly hydrophobic hoti h jo gel m migrate nhi kr paati y aggregate ho jaati h so resolve Krna difficult hota h gel m
Coomasin brilliant blue less sensitive hota h microgram protein ki staining kr skta h
More sensitive AgNO3 use krenge silver dye 2 tk nanogram protein k liye use kr skte h iske alawa fluroscent dye bhi use kr skte h
Kuch protein ki size jyada hoti yaa shape abnormal hota h vo PAGE m move nhi kr paati h
Kavita choudhary
August 17, 2025Answer is d all of the above
Kajal
August 17, 2025All of the above are the issue for 2D PAGE
Avni
August 17, 2025The correct answer is (d)
Shrddha Modi
August 17, 2025D all the problems over there
Simran Saini
August 18, 2025All of the above are issues with 2D-PAGE.
Khushi Vaishnav
August 18, 2025All of the above are issues with 2D-PAGE
Varsha Tatla
August 19, 2025Clear 👍
Bhavana kankhedia
August 24, 2025all of the above are the issue for 2D-PAGE .
Bharti Yadav
August 24, 2025all of the above are issue in 2 D gel technique
Priyanka Choudhary
August 24, 2025All of the above are issued in 2d gel ,and 2D PAGE separates on the basis of charge and molecular size of the protein
Urmi sisodiya
August 24, 2025All of the above
Sonam Saini
August 24, 2025Option d is right
Alec
August 24, 2025option d – all the above are issues with 2d PAGE.
Niti Tanwar
August 24, 2025Option d
Falguni Prakash
August 24, 2025D
Sakshi
August 24, 2025All of the above is correct
Vijay kumar Meena
August 24, 20254 done ✔️
Ayush Dubey
August 25, 2025All of the above are issues with 2D-PAGE.
Devika
August 25, 2025All of the above are issues with 2D gel
Kashish yadav
August 25, 2025Done sir
sakshi khatri
August 25, 2025d all
Shivani Panwar
August 25, 2025All of the above are issue with 2D- PAGE
Monika meena
August 27, 2025Answer d is correct all of the above are issue with 2D gel technique
Pratibha Jain
August 30, 2025Correct answer is option (4)
All of the above are issued in 2D gel ,and 2D PAGE separates on the basis of charge and molecular size of the protein
Sheikh Aleen
August 30, 2025Option D All of the above are issues with 2D-PAGE.
Anisha Beniwal
August 30, 2025All of the above are issues with 2D-PAGE.
Vanshika Sharma
August 30, 2025All of teh above are issues with 2 D electrophoresis
Akshita Jangid
August 31, 20252D Gel separate the protein based upon 1. charge 2. and mass
Komal Sharma
August 31, 20252D-PAGE (Two-Dimensional Polyacrylamide Gel Electrophoresis) is a powerful technique for separating complex protein mixtures based on both isoelectric point (pI) and molecular weight. All are limitations
Shobha Kanwar
August 31, 2025Answer d ,2D separate on the basis of charge and size
Asha Gurzzar
August 31, 2025D is correct all of them are issues for 2D page
Anisha jakhar
September 3, 2025Option d. All of the above
Muskan Yadav
September 3, 2025All of the above are issues with 2D-PAGE
Somya Yadav
September 5, 2025All of the above
Ajay Sharma
September 6, 2025All of the above
Because cbb staining doesn’t work for small rare cellular proteins
We will have to go for more sensitive staining for example agno3
Membrane proteins are hydrophobic so will not run properly in gel
Large protein will not properly move in gel
Aakansha sharma Sharma
October 1, 2025The correct answer is:
(d) All of the above are issues with 2D-PAGE