Two dimensional gel electrophoresis is a technique for separating proteins
(A) Based on charge
(B) Based on mass
(C) Based on both charge and mass
(D) Based on its pI value
Correct answer: (C) Based on both charge and mass
Explanation:
Two-Dimensional Gel Electrophoresis (2D-PAGE) is a powerful technique used for protein separation by combining two different electrophoretic methods:
-
First Dimension: Isoelectric Focusing (IEF)
- Proteins are separated based on their isoelectric point (pI) (the pH at which the protein has no net charge).
- This means proteins migrate according to charge until they reach their pI value, where they stop moving.
-
Second Dimension: SDS-PAGE
- After IEF, the proteins are further separated using SDS-PAGE, which sorts proteins based on molecular mass.
- SDS denatures the proteins and gives them a uniform negative charge, ensuring separation depends only on size.
Thus, 2D-PAGE separates proteins based on both charge (first dimension) and mass (second dimension).
Why Not the Other Options?
-
(A) Based on charge ❌
- Only the first step (IEF) separates based on charge.
- But 2D-PAGE also includes mass separation in the second step.
-
(B) Based on mass ❌
- Only the second step (SDS-PAGE) separates based on mass.
- Charge separation (IEF) is also crucial in 2D-PAGE.
-
(D) Based on its pI value ❌
- pI (isoelectric point) is used in the first step (IEF), but the second step further separates proteins by mass.
Final Answer:
✅ (C) Based on both charge and mass
99 Comments
Parul
February 25, 2025Direct answer. OK sir
Mahima Sharma
August 24, 2025Charges and mass
Nisha
February 25, 2025Yes
Nisha
February 25, 2025Done
Alina mansuri
February 25, 2025Got it
Ujjwal
February 25, 2025Good
Ujjwal
February 25, 2025Good sir
Ritika Jangir
February 26, 2025Done
Shreeji Charan
February 26, 2025Right answer
Ashwini Shekhawat
February 26, 2025Okay sir
Priya khandal
February 26, 2025Okay sir
Suman bhakar
February 26, 2025Right answer
A.
February 26, 2025😍😍😍
Lokesh kumawat
February 26, 2025Done
Abhilasha
February 26, 2025Got it👍🏻
Beena Meena
February 26, 2025👍
Arushi
February 26, 2025☑️
Shivani meena
February 26, 2025Done ✅
Vikram
February 26, 2025Ok sir 👍
Vikram
February 26, 2025Ok sir 🙏
Jyoti Meena
February 27, 2025Done
Kabeer Narwal
February 27, 2025👍👍
Prami Masih
February 27, 2025Okay sir
yogesh sharma
February 27, 2025Great explanation sir 🙏
yogesh sharma
February 27, 2025Easy
Rohit Meena
February 28, 2025Ha Sir 😂 phle bhi Kya the
Rohit Meena
February 28, 2025Maja aa raha hai
Priya khandal
March 2, 2025Done sir
Shalu Choudhary
March 2, 2025Okk sir
Sumit Sultan
March 3, 2025Ok Sir ✅
Anita choudhary
April 18, 2025✅✅
Aman Choudhary
August 13, 2025option c based on their charge and mass
Mohd juber Ali
August 14, 20252D gel electrophoresis separation techniques based on charge (isoelectric focusing)+ mass( SDS PAGE)
Khushi Agarwal
August 14, 2025Option C is correct
Both size nd charge
Anurag Giri
August 14, 2025Both charge and size
Dharmpal Swami
August 14, 2025Both charge and mass
Priya dhakad
August 14, 2025Option c is. Correct
2D gel electrophoresis separates protein based on charge and mass
Neelam Sharma
August 14, 2025Charge and mass
Anjani sharma
August 14, 2025Answer 3 both
Savita Garwa
August 14, 2025Both charge and mass
Sneha Kumawat
August 14, 20252D gel electrophoresis is based on both charge and mass
Akshita Saini
August 14, 2025Based on both charge and mass
Devik
August 14, 2025C
shruti sharma
August 14, 2025option c
Avni
August 14, 2025Two dimensional gel electrophoresis is a technique for separating proteins Based on both charge and mass
Mansukh Kapoor
August 14, 2025The correct answer is option 3rd
2D gel electrophoresis helps into separation on the basis of charge and mass both
Soniya Shekhawat
August 14, 2025Done sir 👍🏻
Dipti Sharma
August 14, 2025Based on both charge and mass bcz IEF focuses on charge and SDS on mass combines 2D GE.
Nilofar Khan
August 14, 2025Answer is c
Karishma
August 14, 20252D-PAGE separates protein on both charge and mass
Meenakshi Choudhary
August 14, 2025Based on both charge and mass
Simran saini
August 14, 2025Option c is correct answer.
Priti khandal
August 14, 2025Option C is correct sir mass and charge both
Komal Pareek
August 14, 2025option c
Priya khandal
August 14, 2025Based on charge and mass
Deepika Sheoran
August 15, 2025Option C is correct answer
Both charge &mass
Priyanka choudhary
August 15, 2025Both charge and mass 👍
Shivani
August 15, 20252D gel electrophoresis is based on both charge and mass
Mitali saini
August 16, 2025Correct answer: (C) Based on both charge and mass
Divya rani
August 16, 20252D is involved both IEF and SDS page where IEF is based on charge and SDS is based on mass
Aafreen
August 16, 2025Based on both charge (IEF) and mass (SDS)
Payal
August 17, 20252D gel is involved in both charge (IFE) and mass (SDS)
Kavita choudhary
August 17, 2025TGGE is based apon both charge and mass
Kajal
August 17, 2025Based on both charge and mass
Khushi Vaishnav
August 17, 2025Based on both mass(SDS) and charge ( IEF)
Sakshi
August 17, 2025Protein seperated on basis of both charge and mass in 2d page
Sakshi
August 17, 2025Protein separated on basis of both charge and mass in 2D page
Manisha choudhary
August 20, 20252D GEL electrophoresis m only charge only mass k base p seprate nhi hota first step m pi value k base p separate krta h ph at which charge on protein is zero
Second step m sds denge protein ko sds denaturant h jo protein ko denature kr uniformly negative charge provide krega jo positive electrode ki side mass k base pr separate hogi
So c option is correct based on both charges and mass
Komal Sharma
August 21, 2025(C) Based on both charge and mass
Hanshika Bansal
August 23, 2025(c)
Bhavana kankhedia
August 24, 2025based on both charge and mass
Akshita Jangid
August 24, 20252d gel technique separate protein based on both charge and mass
Bharti Yadav
August 24, 2025Based on both charge and mass
Urmi sisodiya
August 24, 2025Both charge and mass
Bcoz 2d is the combination of SDS and isoelectric focusing and in isoelectric focusing the protein get seperate on the basis of charge and in SDS the protein get seperate on the basis of mass if the protein pi value is same
Priyanka Choudhary
August 24, 2025C is correct answer,Based on both charge and mass
Ankita Pareek
August 24, 20252D PAGE separates protein based on both charge and mass
Sonam Saini
August 24, 2025Aavesh aur dravyaman donon per aadharit
Alec
August 24, 2025option c – based on charge as well as mass.
Niti Tanwar
August 24, 2025Both charge and mass
Falguni Prakash
August 24, 2025Based on both charge and masss
Anjali
August 24, 2025Both charge and mass
Kashish yadav
August 25, 2025Based on both charge and mass
sakshi khatri
August 25, 2025both charge and mass
Vijay kumar Meena
August 25, 2025C ✔️
Devika
August 25, 2025Based on both charge and mass
Ayush Dubey
August 25, 2025Based on both charge and mass
Anju
August 25, 2025And:c
On the bases of both charge and mass
Surendra Doodi
August 27, 2025Based on both charge and mass
Pratibha Jain
August 30, 2025Correct answer is option (C) Based on both charge and mass
Sheikh Aleen
August 30, 2025Based on both charge and mass
Anisha Beniwal
August 30, 2025Based on both charge and mass
Vanshika Sharma
August 31, 20252D electrophoresis seprate both on the basis of mass and charge
Shobha Kanwar
August 31, 2025Option c is Correct as 2D gel electrophoresis separates protein based on charge and mass
Asha Gurzzar
September 1, 2025C will be correct for both charge and mass
Ajay Sharma
September 2, 2025Option c
Anisha jakhar
September 3, 2025Based on both charge and mass
Muskan Yadav
September 3, 2025Based on both charge and mass
Somya Yadav
September 5, 2025Based on charge and mass
Aakansha sharma Sharma
October 1, 2025Correct answer: (C) Based on both charge and mass