Reason for Smearing in PCR Amplification of Low Copy Number Plasmid DNA During the amplification of a low copy number plasmid DNA by PCR appropriate primers and polymerase are used. […]
Tag: Polymerase Chain Reaction
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CSIR NET Previous Year Questions on PCR
Degenerated Primers for Amplifying Mouse Gene from Drosophila Sequence
Degenerated Primers for Amplifying Mouse Gene from Drosophila Sequence To amplify the mouse version of a gene whose sequence has been reported for Drosophila but not for mouse the primers […]
CSIR NET Previous Year Questions on PCR
Reason for Specific Point Mutation in PCR Products
Reason for Specific Point Mutation in PCR Products Why specific point mutation appears in all the PCR products during the amplification of a genomic DNA segment of interest. Where the […]
CSIR NET Previous Year Questions on PCR
Reason for Multiple Bands in PCR Amplification
Reason for Multiple Bands in PCR Amplification Why a scholar always gets four bands of 0.5 kb, 1 kb, 1.5 kb and 2.0 kb respectively during PCR amplification of a […]
CSIR NET Previous Year Questions on PCR
Principle of Real-Time PCR
Principle of Real-Time PCR The principle which real time PCR is based on; (a) Amplifying and simultaneously quantifying a target DNA (b) Analyzing the PCR product at a predetermined time […]
CSIR NET Previous Year Questions on PCR
Regulation of Heating and Cooling in PCR
Regulation of Heating and Cooling in PCR The alternate heating and cooling of reaction mixture in a PCR is regulated by (a) A heating element and refrigerant (b) A Peltier […]
CSIR NET Previous Year Questions on PCR
Selecting Primers for DNA Amplification
Selecting Primers for DNA Amplification Choose the pair of primers that will allow amplification of the DNA sequence both two given structures the DNA ? 5’GACCTGTGGAAGC…………..CATACGGGATTGA3′ 3’CTGGACACCTTCG……………GTATGCCCTAACT5′ 1. 5’GACCTGTGGAAGC3′ […]
