Selecting Primers for DNA Amplification
Choose the pair of primers that will allow amplification of the DNA sequence both two given structures the DNA ?
5’GACCTGTGGAAGC…………..CATACGGGATTGA3′
3’CTGGACACCTTCG……………GTATGCCCTAACT5′
1. 5’GACCTGTGGAAGC3′
2. 5’CTGGACACCTTCG3′
3. 5’CATACGGGATTGA3′
4. 5’TCAATCCCGTATG3′
(a) 1 and 2
(b) 1 and 3
(c) 1 and 4
(d) 2 and 3
The correct answer is (c) 1 and 4.
✅ Correct Answer: (c) 1 and 4
Explanation:
To successfully amplify a DNA sequence using PCR (Polymerase Chain Reaction), the following conditions must be met:
- Primers must be complementary to the flanking sequences of the target DNA.
- One primer should bind to the 5′ to 3′ strand of the target DNA, and the other should bind to the 3′ to 5′ strand.
- DNA synthesis occurs in the 5′ to 3′ direction, so the primers should be oriented toward each other.
Given DNA Sequence:
5’GACCTGTGGAAGC…………..CATACGGGATTGA3′
3’CTGGACACCTTCG……………GTATGCCCTAACT5′
Primer Pair Selection:
- Primer 1:
5'GACCTGTGGAAGC3'→ Matches the 5′ end of the sense strand → Acts as the forward primer. - Primer 4:
5'TCAATCCCGTATG3'→ Reverse complement of the antisense strand at the 3′ end → Acts as the reverse primer.
Why Other Options Are Incorrect:
| Option | Explanation | Correct/Incorrect |
|---|---|---|
| (a) 1 and 2 | Both primers are oriented in the same direction → No amplification. | ❌ Incorrect |
| (b) 1 and 3 | Primer 3 is in the wrong orientation for binding to the template strand. | ❌ Incorrect |
| (c) 1 and 4 | Correct orientation and complementarity for DNA amplification. | ✅ Correct |
| (d) 2 and 3 | Primer 2 is oriented in the wrong direction. | ❌ Incorrect |
How PCR Works with the Correct Primers:
- Denaturation: Double-stranded DNA is heated to separate the strands.
- Annealing:
- Primer 1 binds to the 5′ end of the sense strand.
- Primer 4 binds to the 3′ end of the antisense strand.
- Extension: DNA polymerase extends both primers, synthesizing complementary DNA strands.
Primer Orientation and Complementarity:
| Primer | Sequence | Binding Strand | Function |
|---|---|---|---|
| Primer 1 | 5’GACCTGTGGAAGC3′ | Sense strand (5′ → 3′) | Forward Primer |
| Primer 4 | 5’TCAATCCCGTATG3′ | Antisense strand (3′ → 5′) | Reverse Primer |
Applications of PCR-Based Amplification:
✅ 1. Molecular Cloning
- Amplify target genes for insertion into plasmids.
✅ 2. Mutation Analysis
- Detect and analyze genetic mutations.
✅ 3. Pathogen Detection
- Identify bacteria and viruses using gene-specific primers.
✅ 4. Genetic Fingerprinting
- Amplify and analyze variable DNA regions for forensic studies.
Summary:
- The correct primer pair for successful amplification of the DNA sequence is Primer 1 and Primer 4.
- Primer 1 binds to the 5′ end of the sense strand, and Primer 4 binds to the 3′ end of the antisense strand — enabling successful PCR amplification.



89 Comments
shivani meena
March 16, 2025done
Akshay mahawar
March 17, 2025Done 👍
Shobha sharma
August 30, 2025Option c
1 and 4
Suman bhakar
March 20, 2025Done
Parul
March 23, 2025Done sir
Ujjwal
March 26, 2025Done sir
Anita Choudhary
April 19, 2025Done
Roopal Sharma
August 23, 2025There are two types of primers forward primer and reverse primer . Primer 1 and 4 are correct set of primer
Varsha Tatla
August 24, 2025Easily clear
Diksha Chhipa
August 23, 20255prime seq is always same of dna seq nd 3 prime primer seq is complementary to 3prime
Khushi Pareek
August 23, 20251&4
Mahima Sharma
August 24, 20251 and 4th
Neha Yadav
August 24, 2025Primer that are allow amplification of dna sequence are : 1 5′ GACCTGTGGAAGC 3′ ( Primer 1 always same as a 5′ sed of dna )
2. 3′ GTATGCCCTAACT 5′ ( Read always 5′ to 3′ and its always complementary sequence of 3′ prime dna sequence )
Soniya Shekhawat
August 24, 2025Forward primer binds at 5’to 3′ and reverse primer binds at 3’to 5’so `1 and 4 is correct option.
Santosh Saini
August 24, 20255’GACCTGTGGAAGC3′ , 5’TCAATCCCGTATG3′ SO primer 1st and 4th would be perfect, so option C is correct
Mohd juber Ali
August 24, 2025Option (c) 1 &4
5′ end primer same
3′ end ka complementary use krte h (direction 5′ to 3′ )
Aafreen Khan
August 24, 2025Primers must be complementary to the sequences of the target DNA.
Primer 1 is 5’GACCTGTGGAAGC3′
Primer 2 is 5’TCAATCCCGTATG3′
Priya dhakad
August 24, 2025Primer 1 is 5’GACCTGTGGAAGC3′ Sense strand (5′ → 3′)
Primer 4 5’TCAATCCCGTATG3′ Antisense strand (3′ → 5′) so option c is correct
Asha Gurzzar
August 30, 2025Option c is correct because primer must be complementary of the target dna sequence
HIMANI FAUJDAR
August 24, 2025Ans correct answer is 1 and 4 Two types of primer which is forward and reverse primer
Primer 1 -Same as 5’end sequence
Primer 2- complimentary to the 3′ end and read as 5′-3′ end.
Anurag Giri
August 24, 20255’ end primer directly used and 3’ end strand complimaetry use as primer so
5’GACCTGTGGAAGC3′ And 5’TCAATCCCGTATG3
Sakshi yadav
August 24, 2025Option c primer 1 5’GACCTGTGGAAGC3′
Primer 2 is 5’TCAATCCCGTATG3′
Rakesh Dhaka
August 24, 20255′ end us ase same and 3′ end complimentary use karte h so ans is . option 3
Avni
August 24, 2025Primer 1 : 5’GACCTGTGGAAGC3′ → Sense strand (5′ → 3′)
Primer 2 : 5’TCAATCCCGTATG3′ → Antisense strand (3′ → 5′)
Arushi Saini
August 24, 20255’ end directly used as a primer and another is complimentory to the DNA strand
Mansukh Kapoor
August 24, 2025The correct answer is option 3rd (1st and 4th)
P1 is the same as the 5′
P2 is the complementary of the 3′
karishma don
August 24, 2025ans is 1 and 4 as 1 is forward primer and 4 is reverse primer of 1st dna
Khushi Agarwal
August 24, 20251,4 is correct here
On 5′ same sequence use a primer and on 3′ complementary to sequence
Surbhi Rajawat
August 24, 2025Answer is D because for primer designing from DNA strand. We take the 5 prime end sequence as it as. And for the next primer we take complimentary sequence of 3 prime end
Aakanksha Sharma
August 24, 2025Option C 1&4
Dharmpal Swami
August 24, 2025For amplification of dna two primers are required
First obtain by 5’end of target dna
Second obtain by 3’end of complementary dna sequences
Niti Tanwar
August 24, 20251,4 right answer
5′ per same sequence
3′ per complementary sequence
Yashika Choudhary
August 24, 2025Primer5 to 3 synthesis
Dipti Sharma
August 24, 2025Correct answer is c 1 and 4 bcz
One primer at 5′ end is directly used as primer and the other at 3′ end must be complementary.
Priyanka Choudhary
August 24, 2025Correct answer is option c, because of this
P1 is the same as the 5′
P2 is the complementary of the 3′
Aman Choudhary
August 24, 2025Option C is correct
5′ end primer is directly use and the other 3′ end primer is complementary
Payal Gaur
August 24, 20251 and 4
Sneha Kumawat
August 24, 20255’GACCTGTGGAAGC3′ ( PRIMER 1 always same as 5 ‘end of primer
3’GTATGCCCTAACT5′ ( Read always 5’to3′ and it’s always complementary sequence.of 3’prime dna sequence. (5’TCAATCCCGTATG 3’)
Karishma
August 24, 20251 and 4 is right answer .. 5 prime end nueotide is use as a primer.. and 3 prime end ka complimantry sequence .. primer ki trh use hota h
Karishma
August 24, 20251 and 4 is right answer
Shrddha Modi
August 24, 2025Option a
Sheikh Aleen
August 24, 2025Option C -Primer 1 binds to the 5′ end of the sense strand and Primer 4 binds to the 3′ end of the antisense strand.
Divya rani
August 24, 2025C is the answer because we take 5′ end sequence as it is as 1st primer and for 2nd primer we take complementary sequence of the 3′ end but read it in the 5′-3′ direction.
AKANKSHA RAJPUT
August 24, 20251,4 is correct here
On 5′ same sequence use a primer and on 3′ complementary to sequence
Heena
August 24, 2025There are two primers used ,one of which is forward primer i.e used as it is and other is reverse primer that’s complementary to 3’end
Heena Mahlawat
August 24, 20251 and 4
Tanvi Panwar
August 24, 2025Option C is correct that is 1 and 4. First primer is Designed directly by 5’end sequence and second primer is complimentary to the 3’end sequence.
Neelam Sharma
August 24, 20251 and 4
Simran Saini
August 24, 20251 and 4.
5’GACCTGTGGAAGC3′
5’TCAATCCCGTATG3′
MOHIT AKHAND
August 24, 2025Done sir ✅
anjani sharma
August 24, 2025Answer c
5′ is used directly as primer while 3′ end is used complementary
Ayush Dubey
August 24, 20251 and 4.
Sakshi Kanwar
August 24, 20251 and 4
As 1st primer will be same as 5′ end and act as the forward primer.
And 2nd primer will be complementary to 3′ end as the reverse primer.
Bhawna Choudhary
August 24, 2025Correct answer is 1 nd 4 bcz
One primer at 5′ end is directly used as primer and the second at 3′ end must be complementary to seq.
Priyanka Verma
August 24, 2025correct answer is 1 and 4
First primer is Designed directly by 5’end sequence and second primer is complimentary to the 3’end sequence.
Bharti Yadav
August 24, 20251 and 4 is correct
5’ same sequence directly use as primer
3′ should be complimentary sequence
Minal Sethi
August 24, 20251 and 4
1 is the forward primer of first sequence and 4th is the reverse primer of first sequence
Priti khandal
August 25, 20251 and 4 is right
5 ‘ same sequence directly use as a primer and 3’ complimentary sequence
shruti sharma
August 25, 20251 and 4
5 prime use directly use as sequence
3 should be complementary
Divya Mandhania
August 25, 2025Option c primer 1 5’GACCTGTGGAAGC3′
Primer 2 is 5’TCAATCCCGTATG3′
Mitali saini
August 25, 2025The correct answer is (c) 1 and 4.
Vanshika Sharma
August 25, 2025Ans is c bcz 1 and 4 is work as forward and reverse primers
Surendra Doodi
August 25, 20255 prime end direct use as primer and 3 prime end complimentary sequence use as primer
Bhavana kankhedia
August 26, 20251 and 4
Bhavana kankhedia
August 26, 20251 and 4 is right answer
Nilofar Khan
August 26, 2025Correct answer is c
1.5’GACCTGTGGAAGC3′
4. 5’TCAATCCCGTATG3′
Manisha
August 26, 2025Forward and backward primers are used
Devika
August 26, 20251and 4 is correct Best Institute for CSIR NET Life Science Coaching – Let’s Talk Academy”
Rishita
August 26, 2025Done
Mohini
August 27, 2025C) 1 and 4
Neeraj Sharma
August 28, 2025Option C is correct
Kanica Sunwalka
August 28, 20255’end used as a primer
3’end ka complementary krdo and read it as 5′-3′
Kajal
August 28, 2025Option c is correct answer as 5end directly work as primer and 3rd end is complementary
Anisha Beniwal
August 28, 2025Option C is correct
Muskan singodiya
August 28, 2025Option c
1 and 4
Deepika sheoran
August 28, 20251 &4 is correct
5′ same sequence directly use as primer.
3′ should be complimentary sequence.
Sumit Chhimpa
August 29, 2025In PCR for primer designing… We directly use 5 ‘ end as a primer.. & 3′ end is complimentory & read 5′ to 3’
Bhavana kankhedia
August 29, 20251 and 4 is correct.
Parul Yadav
August 29, 2025Forward Primer – Matches the 5’end of the sense strand.
Reverse Primer – Reverse complementary of the antisense strand at the 3’end.
Khushi Singh
August 30, 2025C is correct
Yashika Choudhary
August 30, 2025Complementary
Sonam Saini
August 30, 20255 prime and wale sequence ko as it is sakte hain 3 prime wale and ko complementary ke roop mein lete Hain use 5 prime to 3 prime read Kiya jata hai so right answer is one and three
Aartii sharma
August 31, 2025Option c
Priyanka
August 31, 2025Primer 4 binds to the 3′ end of the antisense strand
Kirti Agarwal
August 31, 2025Opt c
Kajal
August 31, 2025Correct Answer is (c) 1 and 4
Aartii sharma
August 31, 20251 and 4
Konika Naval
September 1, 20251 and 4
Muskan Yadav
September 5, 2025The correct answer is (c) 1 and 4