42. Contents in Column I and II are with respect to bacterial transcriptional regulation. Column I Column II i. Positive regulation by Camp a. Ara operon ii. Short abortive b. […]
Tag: operon pyq
Tag: operon pyq
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Understanding trp-lac Fusion Operon Behavior: Regulation and Expression in E. coli
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- June 13, 2025
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41. In a strain of E.coli, a fusion between the lac and trp operon took place and the new locus structure is shown below. The strain lacks the wild type […]
Transcriptional Regulation of the trp Operon by Tryptophan: Mechanism and Biological Significance
40. Transcriptional regulation of trp operon by tryptophan involves binding of tryptophan to (1) the repressor protein and inhibition of transcription by its interaction with the operator region. (2) RNA […]
Bidirectional Promoter Regulation in E. coli: How Loss of Regulatory Protein Binding Alters Reporter Gene Activity
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- June 13, 2025
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39. A construct (shown below) was generated to access the activity of bidirectional promotor (A and B) which has a common regulatory DNA element (X). The construct was used to […]
Effect of trpL Codon Mutations on Tryptophan Synthetase Activity in E. coli: Graph Interpretation and Molecular Insight
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- June 13, 2025
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38. The following graph represents the expression of tryptophan synthetase (TS) in E. coli cells in absence ( ) or presence ( ) of tryptophan in the medium If the […]
Attenuation in the trp Operon: Impact of Protein Synthesis Inhibition on trpL mRNA Structure in E. coli
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- June 13, 2025
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37. Attenuation is a mechanism involved in the regulation of tryptophan operon in E. coli. When tryptophan levels are high in the cell, region 2 of the trpL is blocked […]
Tryptophan Synthase Activity in E. coli: Regulation When Glucose and Tryptophan Are Present
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- June 13, 2025
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36. E.coli are growing in a medium with glucose as the sole carbon source. Tryptophan suddenly added. The cells continue to grow, and divide every 30 minutes. What will be […]
Deletion of the Leader Sequence in the trp Operon: Effects on Tryptophan Biosynthesis in E. coli
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- June 13, 2025
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35. Deletion of the leader sequence of trp operon of E. coli would result in (1) decreased transcription of trp operon. (2) increased transcription of trp operon. (3) no effect […]
The trp Operon in E. coli: A Model of Repressible Gene Regulation with Double Controls
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- June 13, 2025
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34. The tryptophan operon in E. coli is an example of (1) inducible operon, controlled by a repressor protein and attenuation. (2) repressible operon, controlled by a repressor protein and […]
Attenuation in the Trp Operon: How Bacteria Fine-Tune Tryptophan Synthesis
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- June 13, 2025
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33. During regulation of trp operon by attenuation there is (1) Pre-mature termination of translation (2) pre-mature termination of transcription (3) Termination of replication (4) Ribosome fails to read transcript […]


