In E. coli, though DNA polymerase I (POI l) plays an essential role in the replication process, it is not the major polymerase. Instead, the enzyme responsible for advancement of […]
Tag: DNA REPLICATION CSIR NET PYQ
DNA REPLICATION CSIR NET PYQ
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CSIR NET Life Science Previous Year Questions and Solution on DNA REPLICATION
Which Statement About Bacterial DNA Replication Is Incorrect? Insights into DNAB Helicase, Primase, Okazaki Fragments, and oriC Structure
Which one of the tollowtng statements made about the is INCORRECT (1) The rate of forward movement otDnaB helicase along the template DNA increases 10-fold when DnaBand DNA POI III […]
CSIR NET Life Science Previous Year Questions and Solution on DNA REPLICATION
Which Statements About Bacterial DNA Gyrase Are Not True? Understanding Gyrase Function and Its Role in DNA Replication
The following statements were made about bacterialDNA gyrase, which introduces negative supercoiling inDNA. A. If gyrase activity is inhibited, there is an increase inreplication initiation at the origin. B. If […]
CSIR NET Life Science Previous Year Questions and Solution on DNA REPLICATION
How Okazaki Fragments Are Matured into Continuous DNA Strands: Role of DNA Polymerase I and DNA Ligase
In semi-conservative mode of DNA replication twoparental strands unwind and are used for synthesis of new strands following the rule of complimentary base pairing. Synthesis of complimentary strands requirethat DNA […]
CSIR NET Life Science Previous Year Questions and Solution on DNA REPAIR AND RECOMBINATION
How Semi-Conservative DNA Replication in Eukaryotes Is Demonstrated Using Bromodeoxyuridine (BrdU) Labeling
Semi-conservative mode of DNA replication in eukaryotes can be experimentally demonstrated by using (1) BromodU labeling(2) S35 labelling (3) In situ hybridization (4) G-banding Introduction DNA replication is a […]
CSIR NET Life Science Previous Year Questions and Solution on DNA REPLICATION
Predicting DNA Banding Patterns in CsCl Gradient if DNA Replication Were Conservative: A Meselson-Stahl Perspective
The semi-conservative nature of DNA replication was established by Meselson and Stahl in their classic experiment with bacteria. They grew bacteria in N15-NH4Cl containing medium, washed and then incubated in […]
CSIR NET Life Science Previous Year Questions and Solution on DNA REPLICATION
DNA Banding Patterns in CsCl Density Gradient After E. coli Shift from N15 to N14 Medium
E. coli cells were grown in N15 medium for several generations and then shifted to normal medium for one generation. If the DNA isolated from the culture would be centrifuged […]
CSIR NET Life Science Previous Year Questions and Solution on DNA REPLICATION
How 15N/14N Density Gradient Experiment Demonstrates Semi-Conservative DNA Replication
You have labelled DNA in a bacterium by flowing cells in medium containing either 14N nitrogen or the heavier isotope, 15N. Furthermore, you have isolated pure DNA from these organisms, […]
CSIR NET Life Science Previous Year Questions and Solution on DNA REPLICATION
How DNA Polymerases Correct Replication Errors Using 3′→5′ Exonuclease Proofreading Activity
11. Copying errors occurring during replication are corrected by the proof reading activity of DNA polymerases that recognize incorrect bases (1) at the 5′ end of the growing chain and […]
CSIR NET Life Science Previous Year Questions and Solution on DNA REPLICATION
Direction of DnaB Helicase Translocation During E. coli DNA Replication
10. In E. coli, the major DNA unwinding protein helicase, DnaB translocate in (1) 5’à3′ direction along the lagging strand template. (2) 3’à5 ‘direction along the lagging strand template. (3) […]
