While replicating DNA, the rate of mis-incorporation by DNA polymerase is 1 in 105 nucleotides. However, the actual error rate in the replicated DNA is 1 in 109nucleotides incorporated. This […]
Tag: DNA REPLICATION CSIR NET PYQ
Tag: DNA REPLICATION CSIR NET PYQ
DNA REPLICATION CSIR NET PYQ
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Helicase Loading in Eukaryotic DNA Replication Occurs During G1 Phase: Mechanism and Regulation Explained
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- June 14, 2025
- 33 Comments
In eukaryotic replication, helicase loading occurs at all replicators during (1) Go phase (2) G1 phase (3) S phase (4) G2 phase Introduction DNA replication in eukaryotic cells is […]
Which Activity Is Absent in Bacterial DNA Polymerase I?
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- June 14, 2025
- 26 Comments
Among the following which activity is absent in bacterial DNA polymerase I (1) 5’-3’ Polymerase activity (2) 3’-5’ Polymerase activity (3) 5′-3’ Exonuclease activity (4) 3′-5′ Exonuclease activity Introduction […]
What Is the Proofreading Activity of DNA Polymerase? Exploring the Essential 3′→5′ Exonuclease Function
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- June 14, 2025
- 27 Comments
Among the following which iS termed as proof reading activity of DNA polymerase? (1) 5’à3′ polymerase activity (2) 3’à5’ polymerase activity (3) 5’à3′ exonuclease activity (4) 3’à5’ exonuclease activity […]
The Proofreading Mechanism of DNA Polymerase I in E. coli: Role of 3′→5′ Exonuclease Activity
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- June 14, 2025
- 28 Comments
Type of proofreading activity bv DNA polymerase I in E.coli is (1) 5’-3’ exonuclease (2) 5′-3′ endonuclease (3) 3′-5′ exonuclease (4) 3′-5′ endonuclease Introduction DNA Polymerase I (Pol I) […]
How Dideoxycytidine Triphosphate and Dideoxy CMP Affect DNA Synthesis Rate in In Vitro Reactions
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- June 14, 2025
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Double stranded DNA replicates in a semi-conservative manner. In an in vitro DNA synthesis reaction, dideoxyCTP and, dideoxy CMP were individually added in excess (in separate reaction tubes) in addition […]
How DNA Polymerase I Synthesizes DNA on Different DNA Substrates: Single-Stranded, Nicked, Linear, and Circular Molecules
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- June 14, 2025
- 12 Comments
In a cell free extract containing DNA polymerase I, Mg2+, dATP, dGTP, dCTP and dTTP3H), the following DNA molecules were added: Single stranded closed circular DNA moleculecontaining 824 nucleotides. Single […]
Which Statement About DNA Replication Is Incorrect? Insights into Replication Continuity, Origins, and Methylation
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- June 14, 2025
- 25 Comments
Which one of the following statements about DNA replication is INCORRECT? (1) Once DNA replication commences, it always continues uninterrupted until the entire process is complete. (2) Eukaryotic genomes replicate […]
Which Statement About DNA Replication Is Incorrect? Clarifying Leading and Lagging Strand Synthesis
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- June 14, 2025
- 31 Comments
Which one of the following statements about DNA replication is INCORRECT? (1) During DNA replication, When adjacent bidirectional forks converge, the lagging strandwill meet the leading strand of the same […]
Understanding DNA Polymerase’s Selectivity: Why rNTPs Are Incorporated Into DNA at Much Lower Rates Than dNTPs
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- June 14, 2025
- 17 Comments
Although ribonucleoside triphosphates (rNTPs) are present at approximately IO-fold higher concentration than deoxyribo-nucleoside triphosphates (dNTPs) in the cell but they are incorporated into DNA at a rate that is more […]


