52. Enzyme-linked immunospot (ELISPOT) assay measures: (a)Cytokine concentration in culture supernatant (b)Expression of cytokine gene (c)Intracellular cytokine concentration (d)Number of cytokine releasing cells at single cell level Article: Introduction The […]
Tag: dbt jrf pyq 2021
Tag: dbt jrf pyq 2021
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Why You Don’t Need Topoisomerase in a PCR Reaction Mix for Gene Amplification
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- April 13, 2025
- 4 Comments
51. DNA replication requires DNA-Topoisomerase to remove the supercoiling of DNA that accumulates at the end of a growing replication fork. You wish to perform a PCR amplification of a […]
Which Aminoacyl-tRNA Binds First to the P Site During Bacterial Translation?
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- April 13, 2025
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50. During translation which one of the following aminoacyl-tRNA binds first to the P site of bacterial ribosomes? (a) fMet-tRNAfMet (b) fMet-tRNAMet (c) Met-tRNAfMet (d) Met-tRNAMet Article: Introduction In the […]
What Is the Shortest Land Route Between Mumbai and Mexico on the Same Latitude?
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- April 13, 2025
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49. The shortest land route has to be determined from Mumbai to a city X in Mexico at the same latitude. The route will be: (a)A line curving so that […]
Understanding the Conversion of UDP-Galactose to UDP-Glucose: A Key Biochemical Reaction
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- April 13, 2025
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47. Conversion of UDP-Galactose to UDP-Glucose occurs by: (a)Dehydration and hydration (b)Oxidation and reduction (c)Oxidative addition (d)Reductive elimination Biochemical Conversion of UDP-Galactose to UDP-Glucose The conversion of UDP-Galactose to […]
Biochemical Conversion of L-Pyruvate to L-Lactate
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- April 13, 2025
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46. Conversion of L-pyruvate to L-lactate is an example of: (a)Isomerisation (b)Oxidation (c)Reduction (d)Transesterification Biochemical Conversion of L-Pyruvate to L-Lactate The conversion of L-pyruvate to L-lactate is a critical biochemical […]
Understanding DNA Polymerase: Key Functions in DNA Replication
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- April 13, 2025
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45. During replicationDNA polymerase- (a)Can initiate DNA synthesis de novo (b)Cannot initiate DNA synthesis de novo (c)Synthesizes DNA in 3´ to 5´ direction (d)Unwinds DNA in a 5´ to 3´ […]
How Coughing Facilitates the Transmission of Infectious Diseases
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- April 13, 2025
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43. Which one among the following can be effectively transmitted from person to person due to coughing? (a)AIDS & Tuberculosis (b)Dengue & COVID-19 (c)Malaria & Typhoid (d)Swine Flu & Adenovirus […]
Flavin as a Two-Electron Carrier in the Electron Transport Chain
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- April 13, 2025
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39. In the electron transport chain which one of the following can be a two-electron carrier? (a)Cupro proteins (b)Cytochrome (c)Flavin (d)Iron-sulphur proteins (Fe-S cluster) Article: Introduction The electron transport chain […]
Why Bacteria Are Most Sensitive to Ampicillin During the Log Phase of Growth
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- April 13, 2025
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36. At which phase of the growth curve are bacteria more sensitive to ampicillin? Options:- (a) Declining phase (b) Lag phase (c) Log phase (d) Stationary Article: Introduction Antibiotics like […]


