CSIR NET Life Science Previous Year Questions on applied biology Archives - Page 11 of 19 - CSIR NET LIFE SCIENCE COACHING | NTA NET LIFE SCIENCE

3. A transgenic plant is developed with the following T- DNA construct In order to analyze the nature of integration, genomic DNA digested with EcoRl was used for Southern hybridization using either probe A or B. The result obtained is as The following conclusions were made: A. There are two copies of the T-DNA cassette integrated at one loci and a third copy at another loci. B. There are two copies of the T-DNA cassette integrated at one loci. C. Complete T-DNA cassette has been integrated in all cases. D. In one T-DNA cassette there is a truncation towards the RB E. In one T-DNA cassette there is a towards the LB F. The arrangement of the T-DNA cassettes integrated at the same loci is G. The arrangement of the T-DNA cassette integrated at the same loci is Which of the above are correct? (1) B, E and G (2) B, D and F (3) A, D and F (4) B, C and F

CSIR NET Life Science Previous Year Questions and Solution on applied biology

Interpreting Southern blot patterns to infer T-DNA copy number, truncation and arrangement

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November 28, 2025
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A transgenic plant is developed with the following T- DNA construct In order to analyze the nature of integration, genomic DNA digested with EcoRl was used for Southern hybridization using […]

2. Given below are a few approaches/techniques used in the analysis of plants: A. Phenotype linked to the marker gene. B. PCR using primers specific to the host genome. C. Southern hybridization D. PCR using transgene-specific primers. Which one of the following combination of approaches/ techniques can be used to differentiate between transgenic and non-transgenic plants of a particular variety if the site of insertion is unknown? (1) B and C only (2) A and D only (3) A, C and D (4) A, B and C

CSIR NET Life Science Previous Year Questions and Solution on applied biology

How to distinguish transgenic from non-transgenic plants when the insertion site is unknown

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November 28, 2025
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Given below are a few approaches/techniques used in the analysis of plants: A. Phenotype linked to the marker gene. B. PCR using primers specific to the host genome. C. Southern […]

1. During transgenesis, the location of the genes and their number integrated into the genome of the transgenic animal are random. It is often necessary to determine the copy number of genes and their tissue-specific transcription. The following are the possible methods used for the determination. A. Polymerase Chain Reaction (PCR) B. Southern blot hybridization C. Reverse Transcriptase PCR D. Western blot Choose the correct set of combinations. (1) A and B (2) B and C (3) B and D (4) A and D

CSIR NET Life Science Previous Year Questions and Solution on applied biology

Methods to determine copy number and tissue-specific expression in transgenic animals

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November 28, 2025
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During transgenesis, the location of the genes and their number integrated into the genome of the transgenic animal are random. It is often necessary to determine the copy number of […]

57. A group of researchers are testing two new agents, M1 and M2 for their efficacy in selecting transgenic plants. When they performed tissue culture experiments using three explants, A, B, and C without Agrobacterium transformation, and selected the regenerated plants on M1 and M2, the following regeneration frequencies were obtained. Based on the above data, which one of the following conclusions is INCORRECT? (1) M2 is a stronger selection agent than M1 for explant types A and B. (2) A concentration of 100 mg/L of M1 and 15 mg/L of M2 can be used for selection of transformed cells using explant type A. (3) Among the three explants, type C is least suitable at the tested concentrations of M1 and M2 (4) Use of M1 with explant type B is the most ideal combination for selection of transformants.

CSIR NET Life Science Previous Year Questions and Solution on applied biology

Interpreting selection-agent efficacy and explant suitability from regeneration data

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November 28, 2025
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A group of researchers are testing two new agents, M1 and M2 for their efficacy in selecting transgenic plants. When they performed tissue culture experiments using three explants, A, B, […]

56. RNA silencing is an important strategy to control viral infection in plants. The following statements were made regarding RNA silencing. A. It is driven by small interfering RNA (SiRNA) derived from double-stranded form of viral RNA. B. siRNA requires RISC for it function. C. Many plant viruses encode proteins that act as suppressors of RNA silencing. D. Viral P19 protein activates RNA induced silencing complex Which one of the following combination of statements is correct? (1) A, B and C (2) B, C and D (3) A, C and D (4) A, B and D

CSIR NET Life Science Previous Year Questions and Solution on applied biology

How RNA silencing controls plant viruses and the role of P19

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November 28, 2025
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RNA silencing is an important strategy to control viral infection in plants. The following statements were made regarding RNA silencing. A. It is driven by small interfering RNA (SiRNA) derived […]

55. In an attempt to increase the yield of a commercially important enzyme from natural isolate several strategies were adopted as follows: (A) Genome was selectively modified to increase yield. (B) Reappraisal of culture requirements of the modified organism to increase yield. (C) Induced mutants were screened and selected for organism synthesizing improved levels of the enzyme. (D) Organism was genetically modified, so that it produces a factor that enhances stability of the enzyme. Which one of the following options represents strategies that are appropriate for the purpose? (1) A, B, C and D (2) B and C only (3) A, C and D only (4) A and B only

CSIR NET Life Science Previous Year Questions and Solution on applied biology

Strategies to increase yield of a commercially important enzyme from a natural isolate

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November 28, 2025
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In an attempt to increase the yield of a commercially important enzyme from natural isolate several strategies were adopted as follows: (A) Genome was selectively modified to increase yield. (B) […]

54. The figure below depicts a hypothetical scheme for synthesizing a target product in plants. A, B, and C are the precursors of a target product D, whereas E is a by-product. The key enzymes of the pathway are indicated as E1—E6. To enhance the levels of target product D, following strategies were tested: (A) Enhancing the activity of the enzyme E5 by over- expression and/or protein engineering. (B) Enhancing the activity of the enzyme E4 by over- expression and/or protein engineering. (C) Enhancing the levels of C. (D) Blocking the activity of E6 by RNA-interference or CRISPR/Cas-mediated knockout. Which of the above mentioned strategies are likely to provide the maximum enhancement of the target product compared to the by-product, if no feedback regulation exists for any of the enzymes in the pathway? (1) A and B (2) B and C (3) C and D (4) A and D

CSIR NET Life Science Previous Year Questions and Solution on applied biology

Metabolic engineering strategies to maximize target product over by‑product

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November 28, 2025
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The figure below depicts a hypothetical scheme for synthesizing a target product in plants. A, B, and C are the precursors of a target product D, whereas E is a […]

53. Engineering of metabolic pathways in plants can be achieved by introduction and overexpression of appropriate candidate gene(s) using transgenic technology. The figure given below represents a biochemical pathway in plants where a precursor molecule 'A' is converted in to products 'T' and 'X' through a series of enzymatic reactions. Enzymes 1-5 are involved in this pathway. Scientists attempted to increase the levels of 'X' by introducing additional copy of the gene for enzyme '5' under transcriptional control of a strong constitutive promoter. However, the developed transgenic plants did not display a proportionate increase in the level of 'X'. The following statements were proposed for explaining the above results: (A) Enzyme '4' has greater affinity for D than enzyme '3' (B) Feedback inhibition of enzyme '5' by compound X (C) Substrate limitation for enzyme '5' Which of the above statement could represent probable reasons for NOT obtaining proportionate increase in the amount of 'X' in the transgenic plants? (1) Only C (2) Only A and B (3) Only A (4) A, B and C

CSIR NET Life Science Previous Year Questions and Solution on applied biology

Why does overexpressing a pathway enzyme not give proportional product increase?

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November 28, 2025
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Engineering of metabolic pathways in plants can be achieved by introduction and overexpression of appropriate candidate gene(s) using transgenic technology. The figure given below represents a biochemical pathway in plants […]

52. Given below are a few statements on technologies/concepts related to development of transgenic plants: A. Frequency of genetic transformation is influenced only by the genes of Agrobacterium and not by those of the host plants. B. Transgenic plants containing a single copy of the transgene are preferred over those that contain multiple transgene copies for subsequent genetic analysis. C. Super virulent strains of Agrobacterium can be generated by increasing the copy number of virulence genes. D. A non conditional negative selection marker has to be necessarily used with a strong constitutive promoter for the development of transgenic plants. Which one of the following options represents a combination of all INCORRECT statements? (1) A and D only (2) C only (3) B and C only (4) D only

CSIR NET Life Science Previous Year Questions and Solution on applied biology

Identifying incorrect statements about transgenic plant development

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November 28, 2025
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Given below are a few statements on technologies/concepts related to development of transgenic plants: A. Frequency of genetic transformation is influenced only by the genes of Agrobacterium and not by […]

51. Following statements were given regarding factors influencing variation in expression levels of transgene in transgenic plants: A. Difference in restriction enzyme sites within the T- DNA. B. Difference in copy number of the transgene. C. Variations in site of integration of the T- DNA within the plant genome. D. Presence of multiple promoters within the T-DNA region. Which one of the following options represents a combinations of statements that would NOT lead to variations in transgene expression levels in transgenic plants generated using the same T-DNA/ binary vector? (1) A and C only (2) B only (3) C and D only (4) A and D only

CSIR NET Life Science Previous Year Questions and Solution on applied biology

Which factors do not change transgene expression when using the same T‑DNA vector?

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November 28, 2025
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Following statements were given regarding factors influencing variation in expression levels of transgene in transgenic plants: A. Difference in restriction enzyme sites within the T- DNA. B. Difference in copy […]

Tag: CSIR NET Life Science Previous Year Questions on applied biology

Interpreting Southern blot patterns to infer T-DNA copy number, truncation and arrangement

How to distinguish transgenic from non-transgenic plants when the insertion site is unknown

Methods to determine copy number and tissue-specific expression in transgenic animals

Interpreting selection-agent efficacy and explant suitability from regeneration data

How RNA silencing controls plant viruses and the role of P19

Strategies to increase yield of a commercially important enzyme from a natural isolate

Metabolic engineering strategies to maximize target product over by‑product

Why does overexpressing a pathway enzyme not give proportional product increase?

Identifying incorrect statements about transgenic plant development

Which factors do not change transgene expression when using the same T‑DNA vector?

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