10. A fluorophore when transferred from solvent A to solvent B results in an increase in the number of vibrational states in the ground state without any change in the […]
Tag: CSIR NET Life Science Previous Year Questions and Solutions Spectroscopy
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CSIR NET Life Science Previous Year Questions and Solutions Spectroscopy
Optical Density of DNA Solution Numerical
9. The optical density (OD) of a 400 base pair long DNA solution (1 mL) was found to be 0.05. How many DNA molecules are present in the solution? Given: […]
CSIR NET Life Science Previous Year Questions and Solutions Spectroscopy
Molar Absorption Coefficient of Phenylalanine
8. Molar absorption coefficient of phenylalanine is 200 M⁻¹ cm⁻¹ at 257 nm. What concentration (g/L) of this amino acid will give an absorption of 1 in a cell of […]
CSIR NET Life Science Previous Year Questions and Solutions Spectroscopy
Calculation of Protein Concentration Using the Biuret Assay
A protein solution (0.2 ml) of unknown concentration was diluted with 0.8 ml of water. To 0.5 ml of this diluted solution 4.5 ml of biuret reagent was added and […]
CSIR NET Life Science Previous Year Questions and Solutions Spectroscopy
Understanding the Units of Molar Extinction Coefficient (ε)
The units of molar extinction coefficient are (1) L mol-1 crn-1 (2) cm-1 mg ml-1 (3) mol-1 mm (4) mol cm ml-1 Correct Answer: (1) L mol⁻¹ cm⁻¹ Topic Explanation: […]
CSIR NET Life Science Previous Year Questions and Solutions Spectroscopy
Calculating Protein Concentration Using UV Absorbance and Molar Extinction Coefficients
A protein has one tryptophan and one, tyrosine in its sequence. Assume molar extinction coefficients at 280 nm of tryptophan and tyrosine as 3000 and 1500 M-1cm-l, respectively. What would […]
CSIR NET Life Science Previous Year Questions and Solutions Spectroscopy
Relationship Between Absorbance and Transmission in Spectrophotometry
What will be the percentage transmission when absorbance is 1, 2 and 3, respectively? (1) 10, 1, 0.1 (2) 1, 10, 100, (3) 0.2, 0.1, 0 (4) 20, 10, 0 […]
CSIR NET Life Science Previous Year Questions and Solutions Spectroscopy
UV Spectroscopy for most sensitive label-free Protein Quantification
Which one of the methods listed below is the most sensitive label-free quantification method for proteins (1) UV spectroscopy (2) Infra-red spectroscopy (3) Raman spectroscopy (4) 13C content of protein […]
CSIR NET Life Science Previous Year Questions and Solutions Spectroscopy
Intrinsic Fluorescence of Protein Is Due to Aromatic amino acids Tryptophan Tyrosine and Phenylalanine
Which one of the following would contribute to intrinsic fluorescence to a protein? (1) aromatic amino acids (2) disulfide bonds (3) charged amino acids (4) branched chain amino acids […]
