Category: GATE Biotechnology Previous Year Papers with Solutions

Q.65 A bacterial strain is grown in nutrient medium at 37 °C under aerobic conditions. The medium is inoculated with 102 cells from a seed culture. If the number of […]

Q.64 A circular plasmid has three different but unique restriction sites for enzymes ‘a’, ‘b’ and ‘c.’ When enzymes ‘a’ and ‘b’ are used together, two fragments of equal size […]

Q.63 A pilot sterilization was carried out in a vessel containing 100 m3 medium with an initial spore concentration of 108 spores/ml. The accepted level of contamination after sterilization is […]

Q.61 Consider a piston-cylinder assembly shown in the figure below. The walls of the cylinder are insulated. The cylinder contains 1 mole of an ideal gas at 300 K and […]

Q.60 Liquid-phase mass transfer coefficient (kL) is measured in a stirred tank vessel using steady-state method by sparging air. Oxygen uptake by the microorganism is measured. The bulk concentration of […]

Q.59 An enzyme catalyzes the conversion of substrate A into product B. The rate equation for this reaction is −rA = CA / (5 + CA) mol L−1 min−1 Substrate […]

Q.58 The amount of biomass in a reactor at the end of the batch process is 50 g. Fed-batch operation is initiated by feeding the substrate solution at a constant […]

Q.57 Figure below depicts simplified metabolic and transport reactions taking place in the production of B from A in a cell. The subscript ‘i’ refers to intracellular metabolites. rj is […]

Q.56 For x₁ > 0 and x₂ > 0, the value of limx₁ → x₂ (x₁ − x₂) / [x₂ ln(x₁ / x₂)] is ___________. Problem Statement Evaluate the limit: […]