17. Promoters for RNA polymerase III are located at
(1) +1 to +10
(2) -35 to -10
(3) With in transcribed sequence
(4) More than 100 by upstream

The correct answer is (3) Within transcribed sequence.

Promoters for RNA Polymerase III: Location and Function

RNA polymerase III transcribes genes such as tRNA and 5S rRNA. A unique feature of many RNA polymerase III-transcribed genes is that their promoters are located within the transcribed region of the gene, not upstream as is typical for most other genes.

• For tRNA and 5S rRNA genes, the promoter elements (called internal control regions) are found inside the coding sequence rather than in the flanking DNA upstream of the transcription start site.

• This internal location is essential for the binding of transcription factors (like TFIIIC and TFIIIB), which then recruit RNA polymerase III to the gene for transcription initiation.

Supporting evidence:

“The promoters of 5S rRNA and tRNA genes are downstream of the transcription initiation site
“This may be related, at least in part, to the transcription factors bound at the internal promoter regions. Pol III uses also a specific negative regulator, Maf1, which binds to polymerase under stress conditions.
“The type 1 promoter of the Xenopus laevis 5S RNA gene consists of an internal control region (ICR)

Other options explained:

• (1) +1 to +10: This region is just downstream of the transcription start site, not where Pol III promoters are typically found.

• (2) –35 to –10: This is the classic region for bacterial promoters, not eukaryotic Pol III promoters.

• (4) More than 100 bp upstream: Not the case for Pol III; this is more typical for some Pol II regulatory elements.

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