Q.65 The following are important steps in plant tissue culture technique, which is used to multiply transgenic plants.
A. Acclimatization of hardened plants
B. Spray of insecticide
C. Surface sterilization of explant
D. Inoculation of explant on culture medium
Choose the correct answer from the options given below:
- C, D, A only
- B, C, D only
- D, B, A only
- C, A, B only
C, D, and A are essential steps in plant tissue culture for multiplying transgenic plants, while B is not. Surface sterilization prevents contamination, inoculation initiates growth on nutrient media, and acclimatization transitions regenerated plants to soil.
Correct Option: C, D, A only
Plant tissue culture steps like surface sterilization of explant, inoculation on culture medium, and acclimatization of hardened plants are key biotechnology techniques for multiplying transgenic plants. This detailed analysis of a competitive exam question (e.g., GATE Life Sciences) verifies each option, aiding students in molecular biology and plant sciences.
Surface Sterilization of Explant
Surface sterilization of explant is a critical first step to eliminate microbes from plant tissues using agents like sodium hypochlorite or ethanol, ensuring aseptic conditions. Without it, contamination ruins cultures; it’s mandatory before any inoculation.
Inoculation on Culture Medium
Inoculation of explant on culture medium follows sterilization, where the clean explant is placed on nutrient agar with hormones like auxins and cytokinins to induce callus, shoots, or roots. This initiates regeneration in transgenic plant production.
Acclimatization of Hardened Plants
Acclimatization of hardened plants is the final step, gradually exposing in vitro regenerated plants to natural humidity, light, and soil to survive ex vitro conditions. It follows rooting and ensures viable transgenic plantlets.
Why Not Spray of Insecticide?
Spray of insecticide is irrelevant in sterile plant tissue culture environments, as cultures are grown in vitro under controlled, aseptic conditions without pest exposure. It’s a field practice, not a lab technique for transgenic multiplication.
