Q71.Match List I with List II

LIST I
A. pBR322
B. MS medium
C. PCR reaction
D. Protoplast culture

LIST II
I. EC Cockings
II. Kary Mullis
III. Bolivar and Rodriguez
IV. Murashige and Skoog

Choose the correct answer from the options given below:

1. A-IV, B-III, C-II, D-I

2. A-I, B-II, C-IV, D-III

3. A-IV, B-I, C-III, D-II

4. A-III, B-IV, C-II, D-I

   The correct matching for Q71 is A-III, B-IV, C-II, D-I.

   Detailed Matching Explanation

   • A. pBR322 → III. Bolivar and Rodriguez: pBR322 is the first widely used artificial plasmid cloning vector (4361 bp), developed in 1977 by Francisco Bolivar and Raymond Rodriguez in Herbert Boyer’s lab.

   • B. MS medium → IV. Murashige and Skoog: Murashige and Skoog medium (1962) is a nutrient formulation for plant tissue culture, optimized for shoot regeneration with high cytokinin.[ from prior]

   • C. PCR reaction → II. Kary Mullis: Polymerase Chain Reaction (PCR) was invented by Kary Mullis in 1983, enabling exponential DNA amplification via Taq polymerase.[ from prior]

   • D. Protoplast culture → I. EC Cocking: E.C. Cocking pioneered plant protoplast isolation and culture in 1960, removing cell walls for fusion and regeneration.[ from prior]

   Option Analysis

   Option	A-pBR322	B-MS medium	C-PCR	D-Protoplast	Status
   A-IV, B-III, C-II, D-I	Murashige-Skoog	Bolivar-Rodriguez	Kary Mullis	EC Cocking	Wrong (A,B swapped) ​
   A-I, B-II, C-IV, D-III	EC Cocking	Kary Mullis	Murashige-Skoog	Bolivar-Rodriguez	Wrong (all mismatched) ​
   A-IV, B-I, C-III, D-II	Murashige-Skoog	EC Cocking	Bolivar-Rodriguez	Kary Mullis	Wrong (C,D swapped) ​
   A-III, B-IV, C-II, D-I	Bolivar-Rodriguez	Murashige-Skoog	Kary Mullis	EC Cocking	Correct

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   pBR322 MS medium PCR protoplast culture matching questions test recombinant DNA technology and plant biotechnology inventors, key for NEET/CSIR-UGC exams.

   Core Concepts

   These techniques revolutionized molecular biology:

   • pBR322: Plasmid vector with AmpR/TetR markers, unique restriction sites.​

   • MS medium: Basal salts, vitamins for tobacco explants.[ prior]

   • PCR: Thermal cycling (denature-anneal-extend).[ prior]

   • Protoplast culture: Cellulase/pectinase for wall removal.[ prior]

   Why This Matching Matters

   Matches inventors to breakthroughs: Bolivar-Rodriguez (vectors), Murashige-Skoog (plant media), Mullis (amplification), Cocking (fusion tech).​

   Technique	Inventor(s)	Year	Application
   pBR322	Bolivar & Rodriguez	1977	Cloning vector ​
   MS medium	Murashige & Skoog	1962	Plant tissue culture
   PCR	Kary Mullis	1983	DNA amplification
   Protoplast	EC Cocking	1960	Somatic hybrids

   Keywords: pBR322 MS medium PCR protoplast culture matching, Bolivar Rodriguez, Kary Mullis, Murashige Skoog, EC Cocking.