Q.28 Match the entries in Group I with those in Group II.
Group I Group II
P. MTT 1. Dihydrofolate reductase
Q. Annexin V 2. Succinate dehydrogenase
R. Methotrexate 3. Microtubules
S. Taxol 4. Phosphatidylserine
(A) P-3, Q-1, R-4, S-2 (B) P-2, Q-4, R-1, S-3
(C) P-2, Q-3, R-4, S-1 (D) P-4, Q-2, R-1, S-3
Zinc finger proteins and related assays involve specific molecular targets in biotechnology. The correct matching is option (B): P-2, Q-4, R-1, S-3, linking each item to its biological function.
Matching Overview
Group I items represent key reagents in cell biology and pharmacology, paired with Group II targets like enzymes and cellular components. Correct pairings reflect standard biochemical assays and drug mechanisms: MTT assesses viability via mitochondrial activity, Annexin V detects apoptosis markers, Methotrexate inhibits folate metabolism, and Taxol stabilizes cytoskeletal structures.
Correct Answer: Option B
Option (B) P-2, Q-4, R-1, S-3 perfectly matches: MTT to Succinate dehydrogenase (2), Annexin V to Phosphatidylserine (4), Methotrexate to Dihydrofolate reductase (1), Taxol to Microtubules (3). This aligns with experimental uses in viability assays and cancer therapy.
Item Explanations
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P. MTT: Measures cell viability by reduction to formazan via mitochondrial succinate dehydrogenase (complex II), not microtubules or other targets.
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Q. Annexin V: Binds externalized phosphatidylserine on apoptotic cell surfaces, a hallmark of early apoptosis, unrelated to enzymes like DHFR.
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R. Methotrexate: Competitive inhibitor of dihydrofolate reductase, blocking tetrahydrofolate synthesis for DNA/RNA production in chemotherapy.
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S. Taxol (Paclitaxel): Binds and stabilizes microtubules, preventing depolymerization and halting mitosis in cancer cells.
Option Analysis
| Option | P-MTT | Q-Annexin V | R-Methotrexate | S-Taxol | Correct? |
|---|---|---|---|---|---|
| (A) | 3 Microtubules | 1 DHFR | 4 Phosphatidylserine | 2 Succinate DH | No |
| (B) | 2 Succinate DH | 4 Phosphatidylserine | 1 DHFR | 3 Microtubules | Yes |
| (C) | 2 Succinate DH | 3 Microtubules | 4 Phosphatidylserine | 1 DHFR | No |
| (D) | 4 Phosphatidylserine | 2 Succinate DH | 1 DHFR | 3 Microtubules | No |
Biological Applications
These tools aid drug screening: MTT for cytotoxicity, Annexin V for apoptosis, Methotrexate for antifolate studies, Taxol for microtubule dynamics in oncology research.
