Q.47 Match Group I with Group II

Options:

(A) P-1, Q-2, R-4, S-3

(B) P-2, Q-3, R-4, S-1

(C) P-2, Q-4, R-3, S-1

(D) P-3, Q-2, R-1, S-4

Introduction

Modern molecular biology relies on specialized techniques, each associated with specific reagents, matrices, or platforms. “Match the following” questions based on technique–reagent relationships are extremely common in competitive exams. This article explains the correct matching with clear reasoning and option-wise analysis.

Step-by-Step Matching Explanation

P. Real Time-PCR → 2. Syber Green

Real-time PCR uses fluorescent dyes to detect amplified DNA in real time. SYBR Green binds double-stranded DNA and emits fluorescence during amplification.

✅ Correct match: P-2

Q. 2-D Electrophoresis → 4. Ampholytes

2-D electrophoresis separates proteins by:

1. Isoelectric focusing (IEF) – requires ampholytes to create a pH gradient

2. SDS-PAGE for molecular weight separation

✅ Correct match: Q-4

R. Affinity Chromatography → 3. Antibody Linked Sepharose Beads

Affinity chromatography relies on specific binding interactions. Antibody-linked sepharose beads are commonly used to purify target proteins.

✅ Correct match: R-3

S. Microarray → 1. Biochips

Microarrays are performed on biochips containing thousands of immobilized DNA probes used for gene expression analysis.

✅ Correct match: S-1

Correct Matching Sequence

P-2, Q-4, R-3, S-1

Correct Answer

✅ Option (C)

Explanation of All Options

Option (A): P-1, Q-2, R-4, S-3

❌ Incorrect

• Real-time PCR does not use biochips

• 2-D electrophoresis does not use SYBR Green

• Microarray does not use antibody-linked beads

Option (B): P-2, Q-3, R-4, S-1

❌ Incorrect

• 2-D electrophoresis does not involve antibody beads

• Affinity chromatography does not use ampholytes

Option (C): P-2, Q-4, R-3, S-1

✔ Correct

• All techniques are accurately matched with their associated reagents or platforms

Option (D): P-3, Q-2, R-1, S-4

❌ Incorrect

• Real-time PCR does not use antibody beads

• Microarray does not involve ampholytes

Quick Memory Tricks

• Real-time PCR → SYBR Green (fluorescence detection)

• 2-D electrophoresis → Ampholytes (pH gradient)

• Affinity chromatography → Antibody beads (specific binding)

• Microarray → Biochips (DNA probes)

Conclusion

The correct matching of molecular biology techniques with their associated reagents and platforms is P-2, Q-4, R-3, S-1, corresponding to Option (C). Mastery of such technique–reagent relationships is essential for success in biotechnology and life-science competitive examinations.