Q68. The recombination frequencies between three genes x, y and z are as follows: x-y: 2.6%, y-z: 1.4% and x-z: 1.2%. Then the gene order is x-z-y x-y-z y-x-z z-x-y

Q68. The recombination frequencies between three genes x, y and z are as follows:

x-y: 2.6%, y-z: 1.4% and x-z: 1.2%. Then the gene order is

  1. x-z-y
  2. x-y-z
  3. y-x-z
  4. z-x-y

    Determining Gene Order from Recombination Frequencies: x-y 2.6%, y-z 1.4%, x-z 1.2%

    Recombination frequencies help map gene order on chromosomes, with lower percentages indicating closer proximity. For genes x, y, and z showing x-y: 2.6%, y-z: 1.4%, and x-z: 1.2%, the correct sequence follows the additive distance principle where the sum of smaller intervals matches the largest observed frequency.

    Correct Answer

    x-z-y

    In this order, x to z is 1.2%, z to y is 1.4%, and their sum (1.2% + 1.4% = 2.6%) exactly matches the x-y frequency, confirming z lies between x and y.

    Option Analysis

    Option Predicted Frequencies Matches Data? Explanation
    x-z-y x-z: 1.2%, z-y: 1.4%, x-y: 2.6% Yes Additive: 1.2 + 1.4 = 2.6%; all observed values align perfectly .
    x-y-z x-y: 2.6%, y-z: 1.4%, x-z: 4.0% No x-z should be 2.6 + 1.4 = 4.0%, but observed is 1.2% .
    y-x-z y-x: 2.6%, x-z: 1.2%, y-z: 3.8% No y-z should be 2.6 + 1.2 = 3.8%, but observed is 1.4% .
    z-x-y z-x: 1.2%, x-y: 2.6%, z-y: 3.8% No z-y should be 1.2 + 2.6 = 3.8%, but observed is 1.4% .

    Key Mapping Principle

    The gene order positions the two closest genes (lowest frequency) as adjacent, with the middle gene splitting the farthest pair additively. Here, x-z (1.2%) is lowest, followed by y-z (1.4%), placing z centrally. This method ensures no discrepancies in three-gene linkage analysis.

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