Electrophoresis of Single Stranded DNA or RNA

In case of electrophoresis of single stranded DNA or RNA, which type of gels are used ?

(a) Renaturing
(b) Denaturing
(c) The routine agarose gel
(d) The routine polyacrylamide gel

 

The correct answer is:

(b) Denaturing

Explanation of electrophoresis of single stranded DNA or RNA

In electrophoresis of single-stranded DNA (ssDNA) or RNA, denaturing gels are used to prevent the formation of secondary structures due to intramolecular base pairing. These secondary structures can affect migration patterns, leading to inaccurate size estimation.

  • Denaturing polyacrylamide gels (such as Urea-PAGE) or denaturing agarose gels (formamide/formaldehyde-based) are commonly used.
  • Denaturing agents like urea or formamide disrupt hydrogen bonding, ensuring that nucleic acids remain in their linear form for proper separation based on size.

Thus, option (b) Denaturing is the correct choice. ✅

 

 

21 Comments
  • Lavanya Sharma
    February 26, 2025

    👍🏻

  • Lavanya Sharma
    February 26, 2025

    The answer is explained nicely .

  • Ujjwal
    February 26, 2025

    Yes sir

  • Ashwini Shekhawat
    February 26, 2025

    Explanation is quite easy to understand

  • Parul
    February 26, 2025

    Sir understood the question.
    In explanation answer is b and in video it’s C

  • Parul
    February 26, 2025

    Sir understood the question.
    Answers differ in Explanation and video.

  • Parul
    February 26, 2025

    Sir understood the question.
    Answers differ

  • Arushi
    February 26, 2025

    Answer c☑️

  • Ritika Jangir
    February 26, 2025

    Done

  • Pooja jat
    February 27, 2025

    Ans c✅️

  • Pooja jat
    February 27, 2025

    C✅️

    • Lokesh kumawat
      February 27, 2025

      C✔️

  • Pooja jat
    February 27, 2025

    Done., c is right

  • Kabeer Narwal
    February 27, 2025

    Ans c

  • Prami Masih
    February 27, 2025

    👍👍👍

  • yogesh sharma
    February 27, 2025

    C is the correct answer

  • yogesh sharma
    February 27, 2025

    Done c

  • Abhilasha
    February 28, 2025

    Done ✅

  • Priya khandal
    March 2, 2025

    Done sir

  • VIKRAM GAHLOT
    March 7, 2025

    👍

  • Anita choudhary
    April 19, 2025

    “C “correct ✅

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