In case of electrophoresis of single stranded DNA or RNA, which type of gels are used ?
(a) Renaturing
(b) Denaturing
(c) The routine agarose gel
(d) The routine polyacrylamide gel
The correct answer is:
(b) Denaturing
Explanation of electrophoresis of single stranded DNA or RNA
In electrophoresis of single-stranded DNA (ssDNA) or RNA, denaturing gels are used to prevent the formation of secondary structures due to intramolecular base pairing. These secondary structures can affect migration patterns, leading to inaccurate size estimation.
- Denaturing polyacrylamide gels (such as Urea-PAGE) or denaturing agarose gels (formamide/formaldehyde-based) are commonly used.
- Denaturing agents like urea or formamide disrupt hydrogen bonding, ensuring that nucleic acids remain in their linear form for proper separation based on size.
Thus, option (b) Denaturing is the correct choice. ✅
82 Comments
Lavanya Sharma
February 26, 2025👍🏻
Mahima Sharma
August 24, 2025Routine agarose gel
Lavanya Sharma
February 26, 2025The answer is explained nicely .
Ujjwal
February 26, 2025Yes sir
Ashwini Shekhawat
February 26, 2025Explanation is quite easy to understand
Parul
February 26, 2025Sir understood the question.
In explanation answer is b and in video it’s C
Parul
February 26, 2025Sir understood the question.
Answers differ in Explanation and video.
Parul
February 26, 2025Sir understood the question.
Answers differ
Arushi
February 26, 2025Answer c☑️
Ritika Jangir
February 26, 2025Done
Pooja jat
February 27, 2025Ans c✅️
Pooja jat
February 27, 2025C✅️
Lokesh kumawat
February 27, 2025C✔️
Pooja jat
February 27, 2025Done., c is right
Kabeer Narwal
February 27, 2025Ans c
Prami Masih
February 27, 2025👍👍👍
yogesh sharma
February 27, 2025C is the correct answer
yogesh sharma
February 27, 2025Done c
Abhilasha
February 28, 2025Done ✅
Priya khandal
March 2, 2025Done sir
VIKRAM GAHLOT
March 7, 2025👍
Anita choudhary
April 19, 2025“C “correct ✅
Khushi Agarwal
August 14, 2025Option b
Denaturing
Dharmpal Swami
August 14, 2025Denaturing
Anjani sharma
August 14, 2025Denaturing
Karishma Don
August 14, 2025DENATURING GELS
Mitali
August 14, 2025option (b) Denaturing is the correct choice.
Roopal Sharma
August 14, 2025Denaturing gels are used for SS dma and rna
Mansukh Kapoor
August 14, 2025The correct answer is option 2nd
Denaturing gel because it prevents the intramolecular bonding of the bases in ssDNA
Roopal Sharma
August 14, 2025Denaturing gels are used for SS dma and rna so that they can’t make secondary structures
Dipti Sharma
August 14, 2025Agarose gel
Aafreen
August 17, 2025ANS-2 Denaturing gel
Deepika Sheoran
August 14, 2025Denaturing.
Monika jangid
August 14, 2025Denaturing
Anju
August 24, 2025Ans: b
Because denatured gel prevent formation of 2nd str
Neelam Sharma
August 14, 2025Denaturing
Parul Yadav
August 14, 2025Electrophoresis of single stranded DNA or RNA is done in Denaturing gels.
Priyanka choudhary
August 14, 2025Right answer is denature❤️
Priti khandal
August 14, 2025Agarose gel
Priya khandal
August 14, 2025C option is right sir
shruti sharma
August 15, 2025option c agrose gel
Kajal
August 15, 2025Denaturing gel
Aafreen
August 17, 2025ANS-1 Denaturing gel
Kavita choudhary
August 17, 2025Denaturing
Manisha gujar
August 17, 2025Video explanation c is correct
Khushi Vaishnav
August 17, 2025Denaturing gel is used
Avni
August 17, 2025Denaturing
Simran Saini
August 18, 2025Denaturing.
Meenakshi Choudhary
August 18, 2025C option is right
Sakshi
August 19, 2025Denaturing gel is correct
Komal Sharma
August 22, 2025In electrophoresis of single-stranded DNA (ssDNA) or RNA, denaturing gels are used to prevent the formation of secondary structures due to intramolecular base pairing. These secondary structures can affect migration patterns, leading to inaccurate size estimation so option B is correct
Bharti Yadav
August 24, 2025Electrophoresis of single stranded DNA or RNA is done in Denaturing gels.
Varsha Tatla
August 24, 2025For sepration of ss dna use rna denaturing gel
Priyanka Choudhary
August 24, 2025Right answer is option b,denature ,
electrophoresis of single stranded DNA or RNA
Ankita Pareek
August 24, 2025Denaturing gel is used in the electrophoresis of single stranded rna or dna to prevent the formation of secondary structure
Urmi sisodiya
August 24, 2025Denaturing gel is used to seperate single strand rna or dna
Priya dhakad
August 24, 2025Denaturing gels are used for SS dna and rna .
Akshita Jangid
August 24, 2025Denaturing gel is used to separate single stranded DNA or RNA
Anil Choudhary
August 24, 2025Denaturing ( easy)
Bhavana kankhedia
August 24, 2025Option b
Denaturing
Sonam Saini
August 24, 2025Denaturation gel is used for single standard DNA and RNA
Alec
August 24, 2025option b- denaturing gel is used for ss dna and rna.
Niti Tanwar
August 24, 2025Denaturing
Aartii sharma
August 24, 2025Agarose gel
Anjali
August 24, 2025Denaturing
Aartii sharma
August 24, 2025Denaturing gel
Falguni Prakash
August 24, 2025B
Kashish yadav
August 25, 2025Denaturing
Ayush Dubey
August 25, 2025Denaturing
sakshi khatri
August 25, 2025done
Pratibha Jain
August 30, 2025The correct answer is option (b) Denaturing
Sheikh Aleen
August 30, 2025Denaturing
Surendra Doodi
August 30, 2025Denaturing is the correct
Anisha Beniwal
August 31, 2025Denaturing
Vanshika Sharma
August 31, 2025Denaturaing gel is used for ss dna and rna
Shobha Kanwar
August 31, 2025The correct answer is option 2nd
Denaturing gel because it prevents the intramolecular bonding of the bases in ssDNA
Aartii sharma
September 1, 2025The routine agarose gel
Anisha jakhar
September 3, 2025(b) Denaturing is the correct option
Muskan Yadav
September 3, 2025Denaturing polyacrylamide gels
Ajay Sharma
September 4, 2025Denaturing to prevent secondary structure formation
Meera Gurjar
September 13, 2025Denaturing
Aakansha sharma Sharma
October 1, 2025The correct answer is:
(b) Denaturing