In case of electrophoresis of single stranded DNA or RNA, which type of gels are used ?
(a) Renaturing
(b) Denaturing
(c) The routine agarose gel
(d) The routine polyacrylamide gel
The correct answer is:
(b) Denaturing
Explanation of electrophoresis of single stranded DNA or RNA
In electrophoresis of single-stranded DNA (ssDNA) or RNA, denaturing gels are used to prevent the formation of secondary structures due to intramolecular base pairing. These secondary structures can affect migration patterns, leading to inaccurate size estimation.
- Denaturing polyacrylamide gels (such as Urea-PAGE) or denaturing agarose gels (formamide/formaldehyde-based) are commonly used.
- Denaturing agents like urea or formamide disrupt hydrogen bonding, ensuring that nucleic acids remain in their linear form for proper separation based on size.
Thus, option (b) Denaturing is the correct choice. ✅
21 Comments
Lavanya Sharma
February 26, 2025👍🏻
Lavanya Sharma
February 26, 2025The answer is explained nicely .
Ujjwal
February 26, 2025Yes sir
Ashwini Shekhawat
February 26, 2025Explanation is quite easy to understand
Parul
February 26, 2025Sir understood the question.
In explanation answer is b and in video it’s C
Parul
February 26, 2025Sir understood the question.
Answers differ in Explanation and video.
Parul
February 26, 2025Sir understood the question.
Answers differ
Arushi
February 26, 2025Answer c☑️
Ritika Jangir
February 26, 2025Done
Pooja jat
February 27, 2025Ans c✅️
Pooja jat
February 27, 2025C✅️
Lokesh kumawat
February 27, 2025C✔️
Pooja jat
February 27, 2025Done., c is right
Kabeer Narwal
February 27, 2025Ans c
Prami Masih
February 27, 2025👍👍👍
yogesh sharma
February 27, 2025C is the correct answer
yogesh sharma
February 27, 2025Done c
Abhilasha
February 28, 2025Done ✅
Priya khandal
March 2, 2025Done sir
VIKRAM GAHLOT
March 7, 2025👍
Anita choudhary
April 19, 2025“C “correct ✅